A method for preparing cyclic platinum nanoparticles using lanreotide acetate as a template
A lanreotide acetate, platinum nanotechnology, applied in the direction of nanotechnology and other directions, can solve the problems of complex preparation process, severe reaction conditions, environmental pollution, etc., and achieve the effects of simple molecular structure, uniform particle size and low cost
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Embodiment 1
[0021] Take 0.25 mg of lanreotide acetate (manufactured by Wuxi Maimertop Biotechnology Co., Ltd.), and dissolve it in 1 mL of hydrochloric acid solution at pH 4.0 to prepare a lanreotide acetate solution, and place the lanreotide acetate solution in a metal bath at 50°C Mix 200 μL of lanreotide acetate solution after the metal bath with 150 μL of 1.0 mM platinum tetrachloride solution (Tianjin Chemical Reagent Factory) and put it into a constant temperature oscillator in a water bath. The controlled speed is 100 rpm and the temperature is 30 °C Incubate for 18 h; finally add 40 μL of 7.5 mM sodium borohydride (produced by Beijing Zhongsheng Huateng Technology Co., Ltd.) dropwise for reduction, the dropping speed is 1-2 drops / min, 20 μL / drop, the reaction temperature is 30 ° C, and the reaction is 10 min , making it slowly change from light yellow to gray black, that is, to obtain lanreotide acetate-cyclic platinum nanoparticles.
[0022] The morphology of ring-shaped platinum...
Embodiment 2
[0024] Take 0.5 mg of lanreotide acetate and dissolve it in 1 mL of hydrochloric acid solution with pH 4.5 to prepare the lanreotide acetate solution. Place the lanreotide acetate solution in a metal bath at 55°C for 25 minutes; The reetide solution was mixed with 150 μL of 2.0 mM platinum tetrachloride solution, and then placed in a constant temperature oscillator in a water bath, and incubated at a temperature of 30°C for 21 h at a controlled speed of 150 rpm; finally, 80 μL of 7.5 mM sodium borohydride was added dropwise for reduction. The speed is 1-2 drops / minute, 20 μL / drop, the reaction temperature is 32°C, and the reaction is carried out for 10 minutes, so that it slowly changes from light yellow to gray black, and lanreotide acetate-cyclic platinum nanoparticles are obtained.
[0025] The morphology of ring-shaped platinum nanoparticles was characterized by transmission electron microscopy, as shown in figure 2 As shown, the ring width of the ring-shaped platinum nan...
Embodiment 3
[0027] Take 1.0 mg of lanreotide acetate and dissolve it in 1 mL of hydrochloric acid solution with a pH of 5.0 to prepare a lanreotide acetate solution. Place the lanreotide acetate solution in a metal bath at 60°C for 30 minutes; The reetide solution was mixed with 150 μL of 4.0 mM platinum tetrachloride solution, and then placed in a constant temperature oscillator in a water bath, and incubated at a temperature of 30°C for 24 hours at a controlled speed of 200 rpm; finally, 120 μL of 7.5 mM sodium borohydride was added dropwise for reduction, and The speed is 1-2 drops / minute, 20 μL / drop, the reaction temperature is 35°C, and the reaction is carried out for 10 minutes, so that it slowly changes from light yellow to gray black, and lanreotide acetate-cyclic platinum nanoparticles are obtained.
[0028] The morphology of ring-shaped platinum nanoparticles was characterized by transmission electron microscopy, as shown in image 3 As shown, the ring width of the ring-shaped p...
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