Breeding method for increasing paenibacillus sugar yield and application thereof

A technology of bacillus and sugar content, applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of high preparation cost, lack of theoretical guidance, difficulty in large-scale production, etc., and achieve improved flocculation. agent yield and improve flocculation efficiency

Active Publication Date: 2016-02-10
NORTHEASTERN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, there are still problems such as lack of theoretical guidance, high preparation cost, and difficulty in large-scale production that urgently need to be solved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The breeding method for improving the sugar yield of Paenibacillus and its application to the sedimentation treatment of fly ash wastewater with a concentration of 4g / L and a particle size of fly ash of 0.050~0.074mm are carried out according to the following process steps:

[0035] (1) Inoculate the starting strain of Paenibacillus into common liquid medium, and activate it for 20 hours at a temperature of 25-30°C, a pH of 7.0, and a shaker speed of 140r / min;

[0036] (2) Centrifuge the activated bacterial solution at a speed of 8000r / min for 15min, discard the supernatant, and dilute the precipitated bacteria with sterile saline to obtain a bacterial suspension with a concentration of 100000cfu / ml;

[0037] (3) Take 15uL of the bacterial suspension in step (2) to make a bacterial film, and use a multifunctional plasma mutagenesis system to mutate it. The mutagenesis conditions are: power supply 300W, working airflow 12L / min, plasma The height between the volume emissi...

Embodiment 2

[0045] The breeding method for improving the sugar yield of Paenibacillus and its application to the sedimentation treatment of fly ash wastewater with a concentration of 10g / L and a fly ash particle size of 0.074~0.150mm are carried out according to the following process steps:

[0046](1) Inoculate the starting strain of Paenibacillus into common liquid medium, and activate it for 30 hours at a temperature of 25-30°C, a pH of 6.55, and a shaker speed of 130r / min;

[0047] (2) Centrifuge the activated bacterial solution for 10 minutes at a speed of 9000r / min, discard the supernatant, and dilute the precipitated bacteria with sterile saline to obtain a bacterial suspension with a concentration of 100000cfu / ml;

[0048] (3) Take 20uL of the bacterial suspension in step (2) to make a bacterial film, and use a multifunctional plasma mutagenesis system to mutate it. The mutagenesis conditions are: power supply 300W, working airflow 12L / min, plasma The height between the volume emi...

Embodiment 3

[0056] The breeding method for improving the sugar yield of Paenibacillus and its application to the sedimentation treatment of pure culture of Nostoc in water are carried out according to the following process steps:

[0057] (1) Inoculate the starting strain of Paenibacillus into common liquid medium, and activate it for 24 hours at a temperature of 25-30°C, a pH of 7.5, and a shaker speed of 160r / min;

[0058] (2) Centrifuge the activated bacterial solution at a speed of 8000r / min for 15min, discard the supernatant, and dilute the precipitated bacteria with sterile saline to obtain a bacterial suspension with a concentration of 100000cfu / ml;

[0059] (3) Take 15uL of the bacterial suspension in step (2) to make a bacterial film, and use a multifunctional plasma mutagenesis system to mutate it. The mutagenesis conditions are: power supply 300W, working airflow 12L / min, plasma The height between the volume emission source and the sample is 5mm, the working gas is nitrogen, an...

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Abstract

The invention provides a breeding method for increasing paenibacillus sugar yield and application thereof. The method includes the steps that (1) paenibacillus original strains are inoculated into a common fluid medium to be activated; (2) bacterium suspension of certain concentration is formed through dilution; (3) after pellicle is formed, induced mutation is performed; (4) the pellicle is arranged inside a common solid medium in a coating mode to be bred; (5) primary screening is performed; (6) the strains subjected to primary screening are inoculated into the common fluid medium to be bred to obtain strains high in sugar yield; (7) the strains obtained through the step (6) are determined to be paenibacillus strains high in sugar yield; (8) the sugar yield genetic stability of the strains high in sugar yield is determined. The breeding method is applied to sedimentation of microalgae in pulverized fuel ash waste water and water bodies. According to the method, a plasma induced mutation technology is utilized for performing mutation breeding on paenibacillus of high-yield microbial flocculants, the flocculation efficiency of paenibacillus is effectively improved, and the yield of flocculants is effectively increased.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a breeding method for improving the sugar production of Paenibacillus and its application. Background technique [0002] Microbial flocculant is a safe and easily biodegradable new water treatment flocculant obtained by enrichment, screening and purification from microorganisms or their secretions by using biotechnology. Microbial flocculant is a natural flocculant. It also has spectral flocculation activity under the premise of ensuring application safety, which provides broad application prospects for water supply and drainage treatment, food processing and fermentation industries that are closely related to human health. [0003] With the massive discharge of industrial waste water, water pollution has become increasingly serious, and water environment problems have aroused widespread concern. Flocculation and sedimentation is a water treatment m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C02F3/34C12R1/01
Inventor 胡筱敏姜彬慧庞宁黄永刚张兰兰孙伟丽张佳琪
Owner NORTHEASTERN UNIV
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