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Preparation method of fishbone collagen

A technology of bone collagen and fish collagen, which is applied in the direction of animal/human protein, connective tissue peptide, specific peptide, etc., can solve the problems of long production cycle, large volume of extraction liquid, high production cost, etc., to facilitate assembly line operation, The production process is simple and the effect of controlling product quality

Inactive Publication Date: 2016-02-10
李国深
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods of producing fish collagen mainly include hot water extraction method, salt method, acid method, alkali method, enzymatic method and other methods; Less; the general process includes the steps of raw material treatment, enzymatic hydrolysis, filtration, concentration and spray drying. The single enzymatic method and acid method have the following disadvantages: high production cost, poor quality of finished products, long production cycle Long, the volume of the extract is large, the enzymatic hydrolysis solution is not completely removed, and the amount of enzyme is large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Raw material pretreatment: put the fish bones in a high-temperature reaction container, and dry them at 120°C for 25 minutes;

[0023] (2) Crushing treatment: Put the dried fish bones into a disc-type supersonic airflow grinder for 6 minutes to form 40-mesh fish bone powder;

[0024] (3) Collagen extraction: Add distilled water 20 times the volume of fish bone powder in a closed container and stir evenly, add neutral protease with 0.05% of the mass of fish bone powder for enzymatic hydrolysis, the enzymatic hydrolysis temperature is 50°C, and the enzymatic hydrolysis time is 3 hours, then add Adjust the pH value of the acetic acid solution to 3, add 0.05% pepsin in the mass of fish bone powder and react at 37°C for 3 hours, cool down to 15°C to remove acetic acid, then stir at 85°C for 40 minutes to inactivate the enzyme, and obtain a crude fish collagen extract;

[0025] (4) Decolorization and deodorization: add the crude fish collagen extract to activated carbon a...

Embodiment 2

[0028] (1) Raw material pretreatment: put the fish bones in a high-temperature reaction container, and dry them at 125°C for 25 minutes;

[0029] (2) Grinding treatment: Put the dried fish bones into a disc-type supersonic airflow grinder for 8 minutes to make 100-mesh fish bone powder;

[0030] (3) Collagen extraction: Add distilled water 25 times the volume of fish bone powder in a closed container and stir evenly, add neutral protease with 0.1% of the mass of fish bone powder for enzymolysis, the enzymatic hydrolysis temperature is 55°C, and the enzymatic hydrolysis time is 4 hours, then add Adjust the pH value of the acetic acid solution to 4, add 0.1% pepsin in the mass of fish bone meal, and react at 37°C for 4 hours, cool down to 15°C to remove acetic acid, and then stir at 90°C for 45 minutes to inactivate the enzyme to obtain a crude fish collagen extract;

[0031] (4) Decolorization and deodorization: add the crude fish collagen extract to activated carbon and stir f...

Embodiment 3

[0034] (1) Raw material pretreatment: Put the fish bones in a high-temperature reaction container, and dry them at 130°C for 25 minutes;

[0035] (2) Crushing treatment: Put the dried fish bones into a disc-type supersonic airflow grinder for 10 minutes to form 180-mesh fish bone powder;

[0036] (3) Collagen extraction: Add distilled water 30 times the volume of fish bone powder in a closed container and stir evenly, add neutral protease with 0.15% of the mass of fish bone powder for enzymolysis, the enzymatic hydrolysis temperature is 60°C, and the enzymatic hydrolysis time is 5 hours, then add Adjust the pH value of the acetic acid solution to 5, add 0.15% pepsin in the mass of fish bone meal, and react at 37°C for 5 hours, cool down to 15°C to remove acetic acid, and then stir at 95°C for 50 minutes to inactivate the enzyme to obtain a crude fish collagen extract;

[0037] (4) Decolorization and deodorization: Add the crude fish collagen extract to activated carbon and sti...

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PUM

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Abstract

The invention discloses a preparation method of a fishbone collagen, which comprises the following steps: (1) raw material pretreatment: carrying out rapid high-temperature drying on a fishbone so as to prevent the loss of nutrients; (2) crushing treatment: grinding the dried fishbone into 40-180-mesh fishbone meal; (3) extraction of collagen: sequentially carrying out enzymolysis and enzyme deactivation on the fishbone meal, so that a fish-collagen crude extracting solution is obtained; and (4) carrying out decoloration, deodorization and drying on the fish-collagen crude extracting solution obtained in the step above, so that the fishbone collagen is obtained. The method is simple and convenient in extraction, green and environmentally friendly; and a fishbone is prepared into a food with nutritional ingredients, and the biological activity of effective components is completely retained, and therefore, obtained collagen products are good in quality.

Description

technical field [0001] The invention belongs to the technical field of biological product preparation, and in particular relates to a preparation method of fish bone collagen. Background technique [0002] Collagen can supplement the nutrients needed by the skin, enhance the activity of collagen in the skin, moisturize the skin, delay aging, beautify, eliminate wrinkles, and nourish hair. Livestock and poultry-derived animal tissues have always been the main way for people to obtain natural collagen and collagen peptides. However, due to the occurrence of diseases such as mad cow disease and foot-and-mouth disease, people have gradually turned to marine organisms to develop collagen. Fish waste can be used as raw materials to produce collagen, which not only improves the quality of collagen, but also expands the source of collagen raw materials, turning waste into treasure and saving resources. [0003] At present, the methods of producing fish collagen mainly include hot w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/14
Inventor 李国深
Owner 李国深