Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fresh-pig-semen preserving long-acting dilution powder, preparing method thereof and application thereof

A fresh and long-acting technology, applied in the field of livestock breeding, can solve the problems affecting the function of sperm plasma membrane, and achieve the effect of reducing operation and management costs, low price and convenient use

Active Publication Date: 2016-02-17
天津市农业科学院
View PDF5 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It may be that the sperm cells lost some important components during the diluent storage, and the protective agent in the diluent may also have a negative impact on the structure and motility of the sperm, thereby affecting the function of the sperm plasma membrane

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fresh-pig-semen preserving long-acting dilution powder, preparing method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Diluent preparation:

[0031] Accurately weigh the following medicines according to the above parts by weight: glucose 37g, sodium oxalate tetraacetate 1.25g, sodium citrate 6g, sodium bicarbonate 1.25g, potassium chloride 0.75g, penicillin 1.4g, streptomycin 1.4 g, cysteine ​​0.1g, polyvinyl alcohol 0.5g, inositol 0.5g. The above-mentioned drugs were accurately weighed into a 1000mL beaker, then 1000mL of double distilled water was added to the beaker, and finally heated, dissolved and equilibrated in a water bath at 37°C for more than 1 hour before use.

Embodiment 2

[0033] Pig semen dilution, packaging and storage:

[0034] After the pig semen is collected, place it at room temperature for a while to reduce the temperature of the semen to about 37°C, and dilute it with the diluent of the present invention according to the original precision, so that the diluted semen density is about 50 million / mL. After dilution, divide the semen into collection bottles, 80mL per bottle. After subpackaging, put the sperm collection bottle at room temperature to cool down slowly for 30-60 minutes, and finally store it in a 17°C constant temperature refrigerator, and turn the sperm collection bottle once every 12 hours in a 17°C constant temperature box to avoid sperm in a state of suspended animation.

Embodiment 3

[0036] Sperm preservation quality testing:

[0037] Sperm motility, vigor and other quality testing, fully shake the semen in the collection bottle to make the sperm in the bottle evenly distributed, take 10uL of semen in a special sperm counting board, cover it with a cover glass, and place it in a 37°C refrigerator. On the stage of the detector, 10 fields of view were randomly selected for analysis after 2 minutes of incubation. The quality of sperm is tested once a day, and when the sperm motility rate is lower than 0.6, the storage is abandoned, so as to obtain the effective storage days of sperm.

[0038] The integrity of the sperm plasma membrane is detected by the principle of hypotonic swelling.

[0039] Preparation of hypotonic solution: Accurately weigh 7.35g of sodium citrate dihydrate and 13.51g of fructose, and dissolve them completely in 1000mL of double distilled water.

[0040] Detection of sperm plasma membrane integrity

[0041] Take 50 μL of semen and mix...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fresh-pig-semen preserving long-acting dilution powder, a preparing method and an application. The fresh-pig-semen preserving long-acting dilution powder is prepared from, by weight, 35 parts to 40 parts of glucose, 1.2 parts to 1.4 parts of oxalic-acid tetracetic acid sodium, 4 parts to 8 parts of sodium citrate, 1.2 parts to 1.4 parts of sodium bicarbonate, 0.7 part to 0.8 part of potassium chloride, 0.4 part to 0.6 part of polyvinyl alcohol, 0.1 part to 0.2 part of cysteine, 0.4 part to 0.6 part of inositol, 1.0 part to 1.5 parts of penicillin and 1.0 part to 1.5 parts of streptomycin. A use method of the fresh-pig-semen preserving long-acting dilution powder includes the steps that after being collected, pig semen is diluted through 1 L of diluent prepared from the dilution powder, the diluted semen can be preserved in a 17-DEG-C constant-temperature refrigerator for 5 days to 7 days, the quality of the semen can still meet the semen deposition requirement, and the semen live rate is higher than 60%. The fresh-pig-semen preserving long-acting dilution powder, the preparing method and the application are achievements obtained through more than 200 times of tests in three years; the application to a pig farm proves that the quality of the fresh-pig-semen preserving long-acting dilution powder is high, insemination capacity is still achieved after the semen is preserved for 5 days, and the effect is stable.

Description

technical field [0001] The invention belongs to the technical field of livestock reproduction, and relates to a long-acting dilution powder for preserving pig fresh semen, a preparation method and application thereof. Background technique [0002] With the promotion and application of pig artificial insemination technology in intensive pig farms, semen preservation technology has been paid more and more attention, and it has increasingly shown that it can reduce the amount of boar breeding and feeding costs, speed up the improvement of pig breeds, and reduce the spread of diseases, etc. Many advantages. For this reason, in recent years, the technology of storing semen at room temperature has been widely used in artificial insemination of pigs. Researchers at home and abroad have carried out a lot of research work on the dilution solution of pig semen storage at room temperature, and developed a dilution solution formula with better application effect. Dilute powders on the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0226
Inventor 崔茂盛安建勇葛慎锋乔家运牟玉莲张春华黄晶晶李奎
Owner 天津市农业科学院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com