Method for extracting and separating 5-O-gallic acid acyl quinine from tea dust

A technology of galloylquinic acid and 5-O-, which is applied in the field of tea extracts, can solve the problems of difficult large-scale production, high product cost, and high requirements for equipment, and achieve simple operation, high recovery rate, and high product quality. low cost effect

Active Publication Date: 2016-02-17
HEFEI UNIV OF TECH
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many methods for extracting and separating tea polyphenols in tea leaves, but except for high-performance liquid chromatography, the products are all mixtures of various phenolic substances, and the monomer compound 5-O-galloyl cannot be directly prepared quinic acid with very low recovery of 5-O-galloylquinic acid
[0005] High-performance liquid chromatography is currently the only method that can be directly used to separate high-purity 5-O-galloylquinic acid, but this method has the disadvantages of high requirements for equipment, high product cost and difficulty in large-scale production. Therefore, there is an urgent need to develop a separation method that is easy to operate and can be scaled up.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting and separating 5-O-gallic acid acyl quinine from tea dust
  • Method for extracting and separating 5-O-gallic acid acyl quinine from tea dust
  • Method for extracting and separating 5-O-gallic acid acyl quinine from tea dust

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of zirconium phosphate:

[0024] 1. Take 3.58g solid ZrOCl 2 ·8H 2 After O was dissolved in 200 ml of deionized water, 7.56 g of solid (NH 4 ) 2 CO 3 , continue to stir until the solution is clear; then add 2.94g of (NH 4 ) 2 HPO 4 , and 1.01 g of cetyltrimethylammonium bromide (CTAB) was added after complete dissolution to obtain a clear reaction solution. Among them, the raw material ZrOCl 2 ·8H 2 O, (NH 4 ) 2 HPO 4 , CTAB and H 2 The molar ratio of O is ZrOCl 2 ·8H 2 O:(NH 4 ) 2 HPO 4 :CTAB:H 2 O=1:2:0.25:1000.

[0025] 2. Transfer the clarified reaction solution to a stainless steel reactor, heat it to 80°C for 2 days, 90°C for 1 day, and 120°C for 1 day; after the reaction, cool the reactor to room temperature, and centrifuge the zirconium phosphate at 5000rpm for 1 day. The solid was washed with deionized water to pH=7, dried in an oven at 80°C for 12 hours, and calcined in a muffle furnace at 550°C for 6 hours to obtain white zi...

Embodiment 2

[0041] 1. Preparation of zirconium phosphate:

[0042] The difference between the preparation method of zirconium phosphate in this example and Example 1 is: the raw material ZrOCl 2 ·8H 2 O, (NH 4 ) 2 HPO 4 , CTAB and H 2 The molar ratio of O is ZrOCl 2 ·8H 2 O:(NH 4 ) 2 HPO 4 :CTAB:H 2 O=1:2.5:0.25:1000, and the remaining operation steps and parameters are the same as those in Example 1.

[0043] 2. Preparation of 5-O-galloquinic acid:

[0044] 1. Take 1 g of Taiping Houkui tea powder that has passed through a 40-mesh sieve, add 10 mL of an ethanol solution with a volume concentration of 70%, and perform ultrasonic extraction at 50W / 45kHz for 60min; the extract is filtered through a 0.45μm membrane to obtain a crude extract.

[0045] 2. Add 0.8g of zirconium phosphate to the crude extract in the ratio of 1ml of crude extract, add the zirconium phosphate obtained in step 1 to the crude extract, under the condition of 37°C / 150rpm, shake and absorb on a shaking shak...

Embodiment 3

[0049] 1. Preparation of zirconium phosphate:

[0050] The difference between the preparation method of zirconium phosphate in this example and Example 1 is: the raw material ZrOCl 2 ·8H 2 O, (NH 4 ) 2 HPO 4 , CTAB and H 2 The molar ratio of O is ZrOCl 2 ·8H 2 O:(NH 4 ) 2 HPO 4 :CTAB:H 2 O=1:1.5:0.25:1000, and the remaining operation steps and parameters are the same as those in Example 1.

[0051] 2. Preparation of 5-O-galloquinic acid:

[0052] 1. Take 1 g of Taiping Houkui tea powder that has passed through a 40-mesh sieve, add 20 mL of ethanol solution with a volume concentration of 70%, and perform ultrasonic extraction at 75W / 45kHz for 40min; the extract is filtered through a 0.45μm membrane to obtain a crude extract.

[0053] 2. Add 0.8g of zirconium phosphate to the crude extract according to the ratio of 1ml of the crude extract. Add the zirconium phosphate obtained in step 1 to the crude extract. Under the condition of 37°C / 150rpm, vibrate and adsorb on a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
purityaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for extracting and separating 5-O-gallic acid acyl quinine from tea dust, which comprises steps: (1) tea dust is taken, and is added with ethanol solution according to the solid-liquid radio of 1g:10-20ml, is extracted through ultrasound, is filtered through a filter film to obtain a crude extraction; (2) the crude extraction is added with zirconium phosphate according to the radio that 1ml crude extraction is added with 0.1-1g zirconium phosphate, is vibrated for 6-24h in a shaker, and is centrifugally separated out zirconium phosphate and is washed through water; (3) the zirconium phosphate which is washed is added with phosphoric acid deionized water solution according to the solid-liquid radio of 0.1g:1ml, is vibrated for 10-24h in the shaker, is centrifugally separated to obtain a desorption solution, and is filtered through a filter film, thereby obtaining the 5-O-gallic acid acyl quinine from tea dust by freezing and drying a filter solution. The method for extracting and separating the 5-O-gallic acid acyl quinine from the tea dust can improve purity and recovery of 5-O-gallic acid acyl quinine, is simple and convenient in operation, green and environmentally friendly and low in demands for production equipment, and is suitable for scale production.

Description

technical field [0001] The present invention relates to tea extract, in particular to a method for separating tea polyphenols from tea leaves. Background technique [0002] Tea polyphenols are the main active components of tea, and are a general term for a class of phenolic substances with anti-aging, cancer prevention, and hypoglycemic and lipid-lowering effects. 5-O-Galloylquinic acid (5-O-Galloylquinic acid) is a specific compound in tea polyphenols, and its chemical structure is shown in the following formula I. 5-O-galloquinic acid has significant antioxidant capacity and is a potential food or cosmetic additive; in terms of disease treatment, studies have shown that 5-O-galloquinic acid is effective in leishmaniasis (kala azar) Has a very good curative effect (L.R.F.deSousa, S.D.Ramalho, J.B.Fernandes, M.F.D.F.daSilva, M.R.D.Iemma, C.J.Correa, D.H.F.deSouza, M.I.S.Lima, P.C.Vieira, LeishmanicidalGalloylquinicAcidsareNoncompetitiveInhibitorsofArginase,JBrazil3818,25(Ch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/56C07C69/84
CPCC07C67/56C07C69/84
Inventor 马意龙商亚芳刘凤茹王彩虹朱丹晔
Owner HEFEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap