Novel alkaloid Oleracone in herba portulacae, and extraction separation method thereof
A separation method, the technology of purslane, applied in the direction of drug combination, non-central analgesic, antipyretic, etc., can solve the problems of separation and pharmacological activity of the new skeleton compound of purslane that have not been reported, and the pharmacological activity is weak
Active Publication Date: 2016-02-17
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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However, there is no report on the separation and pharmacological activity of the new skeleton compound of purslane in the present invention
[0003] At present, most of the chemical components isolated from purslane are known, and their pharmacological activity is relatively weak, so the development and separation of new compounds in purslane is urgently needed
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[0029] The present invention will be further described in detail below in conjunction with specific examples.
[0030] The present embodiment provides a new alkaloid Oleracone in purslane, molecular formula is C 14 h 17 NO 2 , named Oleracone, structural formula please refer to figure 1 .
[0031] see Figure 2-11 , The structure identification and deduction of the new alkaloid Oleracone in purslane of the present invention.
[0032] Yellow powder, easily soluble in chloroform and methanol, insoluble in water. Spray dilute bismuth iodide potassium test solution was positive, [α] 20 D +5.2( c 0.6,MeOH), UV(MeOH)λ max: 360, 255nm, IRν N-H 3425.8, ν =C-H 3193.8, ν C-H 2949.8, ν C=O 1700.2, ν C=C 1590.1, β NH 1548.4, δ NH 1506.8, ESI-MS gives M / Z: 232.1326[M+H] + The quasi-molecular ion peak has a molecular weight of 231.1255. combine 13 CNMR, 1 According to HNMR and DEPT data, it is speculated that the molecular formula of the compound is C 14 h 17 N0 2...
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The invention discloses a novel alkaloid Oleracone in herba portulacae, and an extraction separation method thereof. According to the extraction separation method, dried herba portulacae is subjected to extraction via water boiling; an obtained solution is filtered, filtrates are mixed, and heating condensation is carried out; an obtained mixture is subjected to impurity removing using macroporous resin, and gradient elution with water and ethanol respectively, and an obtained ethanol eluant is subjected to decompress concentration; an obtained concentrate is extracted with ethyl acetate, and ethyl acetate is recycled via decompress concentration; an obtained extract is subjected to silica gel column chromatography separation, is subjected to gradient elution with petroleum ether-acetone respectively so as to obtain 130 parts, and the 90 to 130 parts of eluates are subjected to decompress concentration; 10 parts are obtained via methanol-water gradient elution using an ODS medium pressure column, an obtained part 3 is subjected to decompress concentration, and is subjected to methanol-water gradient elution using Sephadex LH-20 so as to obtain an alkaloid compound with new skeleton. The structure formula of the alkaloid compound is determined via wave spectrum analysis, chemical method, and X-ray single crystal diffraction, and the novel alkaloid is named as Oleracone. The herba portulacae novel alkaloid Oleracone obtained via extraction separation can be taken as a synthesis primer of other compounds, and a raw material used for new drug development and pharmacological activity research.
Description
technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a new skeleton alkaloid extracted, separated and identified from purslane medicinal material and an extraction and separation method thereof. Background technique [0002] purslane( Portulacaoleracea L.) is a plant of the family Portulaca oleracea. The 2015 edition of "Pharmacopoeia of the People's Republic of China" records that the dried aerial part of purslane is used as medicine. Carbuncles, swollen boils, eczema, erysipelas, snake bites, blood in the stool, hemorrhoids, metrorrhagia, etc. Modern pharmacological studies have shown that purslane has antibacterial and antiviral effects, lowering blood pressure, lowering blood lipids, anti-oxidation, anti-cancer, relaxing skeletal and smooth muscles, anti-inflammatory and analgesic, and regulating immune function; these pharmacological effects and its main chemical components include fla...
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Login to View More IPC IPC(8): C07D209/52A61P29/00
CPCC07D209/52
Inventor 英锡相张文洁孟一晗英哲铭
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE