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Method for mixed fermentation of Schizochytrium sp.

A technology of Cryptodinium kouli and mixed fermentation, applied in the field of microbial fermentation, can solve the problems of reduced winterization efficiency, increased production cost, and reduced production efficiency, and achieve the effects of improving oil quality, increasing efficiency, and reducing energy consumption

Active Publication Date: 2018-12-04
GUANGDONG RUNKE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When there are more high-melting point components in the oil, not only more crystals formed by this agglomeration, but also more liquid is entrained inside the crystals, the resulting crystals are unstable, difficult to filter and sometimes even impossible to filter, making The efficiency of winterization is reduced. To achieve the ideal winterization effect, repeated multi-step crystallization operations or other complex crystallization processes may be required. Therefore, the increase in steps and the extension of time will result in increased energy consumption, which is not consistent with energy saving. The requirements and development direction of industrial production are contrary to each other, which reduces the production efficiency and greatly increases the production cost

Method used

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  • Method for mixed fermentation of Schizochytrium sp.
  • Method for mixed fermentation of Schizochytrium sp.
  • Method for mixed fermentation of Schizochytrium sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Production of docosahexaenoic acid by mixed culture of Schizochytrium sp.

[0048] 1. Preparation of seed solution

[0049] Each liter of seed medium contains raw materials of the following qualities:

[0050] Glucose 10g; Peptone 1g; Yeast extract 0.5g; Sodium glutamate 0.5g; Nacl 20g; MgSO 4 ·7H 2 O2g; Kcl 0.5g; (NH 4 ) 2 SO 4 0.1g; Cacl 2 2H 2 0 0.1g; KH 2 PO 4 0.1 g; pH=6.5.

[0051] Preparation of Seed Solution of Cryptidinium koulii

[0052] Under sterile conditions, according to the inoculation amount of 10% by volume, transfer the liquid-preserved strains into a cooled 250-ml Erlenmeyer flask containing 50 ml of sterile seed medium, and cultivate them at 20°C, 100rpm, and in the dark. Carry out the first activation until the cells enter the logarithmic growth phase; then transfer the culture solution into fresh sterile seed medium according to the inoculum volume of 10% by volume, and culture at 20°C, 100rpm, and dark conditions for the second Afte...

Embodiment 2

[0064]Production of docosahexaenoic acid by mixed culture of Schizochytrium sp.

[0065] 1. Preparation of seed solution

[0066] Each liter of seed medium includes the following quality raw materials: glucose 30g; yeast extract 3g; sodium glutamate 2g; peptone 1g; Nacl 15g; MgSO 4 ·7H 2 O 5g; Kcl 1g; (NH 4 ) 2 SO 4 0.2g; Cacl 2 2H 2 0 0.3g; KH 2 PO 4 0.3 g; pH=6.5.

[0067] Preparation of Seed Solution of Cryptidinium koulii

[0068] Under sterile conditions, according to the volume percentage of 20% inoculum, put the liquid preserved Cryptidium koelii into a 250 ml Erlenmeyer flask containing 50 ml of sterile seed medium, at 30°C, 200rpm, under dark conditions Cultivate until the cells enter the logarithmic growth phase to complete the first activation; then add the culture solution into fresh sterile seed medium according to the volume percentage of 20% inoculum, and cultivate at 30°C, 200rpm, and dark until the cells After entering the logarithmic growth phase ...

Embodiment 3

[0080] Production of docosahexaenoic acid by mixed culture of Schizochytrium sp.

[0081] 1. Preparation of seed solution

[0082] Each liter of seed medium contains raw materials of the following qualities:

[0083] Glucose 20g; Yeast extract 2g; Sodium glutamate 1g; Peptone 1g; Nacl 15g; MgSO 4 ·7H 2 O5g; Kcl1g; (NH 4 ) 2 SO 4 0.2g; Cacl 2 2H 2 0 0.3g; KH 2 PO 4 0.3 g; pH=6.5.

[0084] Preparation of Seed Solution of Cryptidinium koulii

[0085] Under sterile conditions, according to the volume percentage of 18% inoculum, the liquid preserved Cryptidium koelii was inserted into a 250-milliliter Erlenmeyer flask containing 50 milliliters of sterile seed medium, at 28°C, 180rpm, and dark conditions Cultivate until the cells enter the logarithmic growth phase to complete the first activation; then transfer the culture solution into fresh sterile seed medium according to the volume percentage of 18% inoculum, and cultivate at 28°C, 180rpm, and dark until the cells ...

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Abstract

The invention relates to microbial fermentation, in particular to a mixed fermentation method for schizochytrium sp. and crypthecodinium cohnii. The method includes the steps that the crypthecodinium cohnii is inoculated in a culture medium containing a carbon source, a nitrogen source and essential nutrient elements to be fermented and prepared into fermentation liquor; when the crypthecodinium cohnii enters in a logarithmic phase, the schizochytrium sp. is inoculated into the fermentation liquor in the step A for conducting mixed fermentation; the crypthecodinium cohnii belongs to the crypthecodinium cohnii algae species capable of accumulating polyunsaturated fatty acids, and belongs to the crypthecodinium cohnii genus; the schizochytrium sp. belongs to the schizochytrium sp. algae species capable of accumulating polyunsaturated fatty acids, and the mass percentage composition of palmitic acid in the schizochytrium sp. is not lower than 30%. By the adoption of the method, the problems that when existing crypthecodinium cohnii and schizochytrium sp. are fermented independently, the technical defects of varying degrees exist, follow-up treatment is complex and the production cost is high are solved, and the advantages that the technological period is short, the production cost is low, prepared products can meet the related standard requirement without complex follow-up treatment and the quality of fat is high are achieved.

Description

technical field [0001] The invention relates to microbial fermentation, in particular to a method for mixed fermentation of Schizochytrium and Cryptidium koii. Background technique [0002] Polyunsaturated fatty acid (PUFA) refers to a straight-chain fatty acid containing two or more double bonds and a carbon chain length of 18-22 carbon atoms. It is an important part of cell biomembranes and is necessary for the human body fatty acid. It plays an extremely important role in life activities. For example, PUFA has the functions of anti-cancer, preventing cardiovascular and cerebrovascular diseases, improving body immunity, delaying aging, and anti-allergy. Among them, docosahexaenoic acid (DHA) has more important physiological functions in PUFA. [0003] DHA is a PUFA of the omega-3 series. Studies have found that DHA mainly exists in the gray matter of the brain. It is the main component of ω-3 PUFA in the brain and retina, and plays a very important role in the brain and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/64C12R1/89
Inventor 姜悦陈峰陈璇苏娇娇
Owner GUANGDONG RUNKE BIOLOGICAL ENG
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