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Extraction technology for water-insoluble plant dietary fiber

A technology of dietary fiber and extraction process, which is applied in the field of plant-based dietary fiber extraction process, can solve the problems of less literature, achieve high purity and reduce environmental pollution

Inactive Publication Date: 2016-03-02
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are far fewer related studies on the extraction of insoluble dietary fiber from bean dregs than on the extraction of soluble dietary fiber from bean dregs, and there are fewer related research reports in the literature

Method used

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  • Extraction technology for water-insoluble plant dietary fiber
  • Extraction technology for water-insoluble plant dietary fiber
  • Extraction technology for water-insoluble plant dietary fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The reagents used in this example are as follows:

[0026] Thermostable α-amylase BR Guangxi Tiandiyang Biological Reagent Co., Ltd.

[0027] The enzyme activity is 300U / mL, the optimum temperature is 100±5℃, and the optimum pH=6.0~7.0.

[0028] Neutral Protease BR Guangzhou Mingyuan Company

[0029] The enzyme activity is 50U / mg, the stable pH is 5.5-8.5, and the optimum temperature is 45℃-50℃. Its vitality is defined as 1 μg tyrosine produced by hydrolyzing casein in 1 min under the conditions of 30°C and pH=7.5, which is expressed in U / g.

[0030]

[0031] Use the above reagents to prepare a solution as follows:

[0032] (1)0.05mol / LMES-TRIS buffer:

[0033] Weigh 4.3021g MES and 2.6928g TRIS, dissolve it with 374mL water, adjust pH=8.17 with 6mol / L sodium hydroxide (the temperature is 25℃ during preparation, calculated by interpolation method), add water and dilute to 440mL.

[0034] (2) Neutral protease solution:

[0035] Use 0.05mol / L MES-TRIS buffer to make a 50mg / mL neutral ...

Embodiment 2

[0074] A water-insoluble plant-based dietary fiber extraction process includes the following steps:

[0075] (1) Drying and crushing the Okara to obtain Okara powder;

[0076] (2) Add 5ml of distilled water and 5ml of pH6.5 phosphate buffer to each gram of okara powder to obtain okara;

[0077] (3) Add neutral protease to the okara pulp for the first enzymolysis, and the amount of neutral protease added is the okara powder described in step (1): neutral protease = 1g:0.7mg to obtain the first enzymolysis okara ;

[0078] (4) Perform the first enzymolysis treatment on the first enzymolysis okara pulp to obtain the first enzymolysis okara pulp;

[0079] (5) Add heat-resistant alpha-amylase to the first enzyme-inactivated okara pulp for the second enzymolysis, and the amount of heat-resistant alpha-amylase added is the okara powder described in step (1): heat-resistant alpha-amylase =1g:10μl to obtain the second enzymolysis okara pulp;

[0080] (6) Perform the second enzymatic inactivatio...

Embodiment 3

[0086] A water-insoluble plant-based dietary fiber extraction process includes the following steps:

[0087] (1) Drying and crushing the Okara to obtain Okara powder;

[0088] (2) Add 7 ml of distilled water and 7 ml of pH 7.0 phosphate buffer to each gram of bean dregs powder to obtain bean dregs pulp;

[0089] (3) Adding a neutral protease to the okara pulp for the first enzymolysis, and the amount of the neutral protease added is the okara powder described in step (1): neutral protease = 1g:1mg to obtain the first enzymolysis okara;

[0090] (4) Perform the first enzymolysis treatment on the first enzymolysis okara pulp to obtain the first enzymolysis okara pulp;

[0091] (5) Add heat-resistant alpha-amylase to the first enzyme-inactivated okara pulp for the second enzymolysis, and the amount of heat-resistant alpha-amylase added is the okara powder described in step (1): heat-resistant alpha-amylase =1g:20μl to obtain the second enzymolysis okara pulp;

[0092] (6) Perform the secon...

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Abstract

An extraction technology for water-insoluble plant dietary fiber comprises the following steps: drying bean dregs and crushing to obtain bean dregs powder; adding distilled water and a phosphoric acid buffer into per one gram of the bean dregs powder, so as to obtain a bean dregs slurry; adding a neutral protease into the bean dregs slurry for first enzymolysis, and then performing first enzyme-killing processing, so as to obtain a first enzyme-killing bean dregs slurry; adding a heat-resistant alpha-amylase into the first enzyme-killing bean dregs slurry for secondary enzymolysis, so as to obtain a second enzymolyzed bean dregs slurry; performing secondary enzyme-killing processing on the second enzymolyzed bean dregs slurry, so as to obtain a second enzyme-killing bean dregs slurry; performing centrifugal separation on the second enzyme-killing bean dregs slurry, filtering and getting the filter residue, washing the filter residue and drying, so as to obtain the water-insoluble dietary fiber in bean dregs. The technology comprehensively considers factors influencing the extraction rate, such as pH value, substrate concentration, crushing degree, enzyme usage condition, enzyme usage amount and the like. The technology enables the bean dregs resource to be reasonably utilized, and reduces environment pollution caused by discarded beans.

Description

Technical field [0001] The invention relates to the field of food processing, in particular to a water-insoluble plant dietary fiber extraction process. Background technique [0002] Dietary fiber refers to polysaccharides that cannot be used by the human body. The digestive enzymes in the human gastrointestinal tract cannot digest dietary fiber, so it is not easily absorbed by the body. Such polysaccharides are mainly derived from complex carbohydrates in plant cell walls, and can also be called non-starch polysaccharides, that is, non-α-glucan polysaccharides. It is divided into soluble dietary fiber and insoluble dietary fiber. The nutritional value of dietary fiber has attracted more and more attention, and its research has also become more and more in-depth. Now dietary fiber is recognized as the first after the six major nutrients of protein, carbohydrate, fat, vitamins, minerals and water. Seven types of nutrients. [0003] China is the hometown of soybeans and has a long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/22
CPCA23V2002/00A23V2250/5116A23V2300/28A23V2300/14
Inventor 吴书建何然张新卿明义
Owner GUANGXI UNIV