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Method for culturing biofloc and method for applying biofloc to aquaculture

A technology of biological flocculation and cultivation methods, applied in the directions of microorganism-based methods, biochemical equipment and methods, biological water/sewage treatment, etc. The effect of reducing carbon source cost and bait cost

Inactive Publication Date: 2016-03-02
YANGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: firstly, the cost of such soluble carbon sources is relatively high, which increases the cost of cultivation and reduces the economic benefits of cultivation, and there are limitations in the extraction of materials for such carbon sources, such as sugarcane molasses being limited to sugarcane production. Second, the microorganisms in the water only rely on their own flocculation and lack of attachment carriers, so it takes a long time (more than two weeks) to generate bioflocs, which increases the time cost; third, the bioflocs obtained by cultivation The size is different, and the size is limited to 100-200 microns. It cannot be ingested by larger economical species, and the bait utilization effect is not good.

Method used

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  • Method for culturing biofloc and method for applying biofloc to aquaculture

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for cultivating bioflocs, comprising the steps of:

[0023] 1) Select the dried wheat straw, crush the straw with a pulverizer, and then screen it with a 20-mesh screen to obtain straw particles with a particle size of less than 0.1mm;

[0024] 2) Mix the above-mentioned straw granules with cornstarch and water evenly according to the mass ratio of 10:0.5:1.5, and extrude the mixture with an extrusion extruder, control the temperature of the extrusion cavity of the extrusion extruder at 120°C, and The pulverizer crushes the expanded mixture particles into solid particles with a particle size of 0.5 mm;

[0025] 3) Mix the above-mentioned solid particles and culture water in the culture unit according to the volume ratio of 1.1:1, and then add the bacillus named Dalianpingdao-22 and the preservation number CCTCCM2015216 into the culture unit, so that the bacillus in the culture unit The number of live bacteria is 3×10 10 cfu / L, maintain the dissolved oxygen in...

Embodiment 2

[0030] A method for cultivating bioflocs, comprising the steps of:

[0031] 1) Choose the rice straw that has been dried in the sun, crush the straw with a pulverizer, and then screen it with a 20-mesh screen to obtain straw particles with a particle size of less than 0.1mm;

[0032] 2) Mix the above-mentioned straw granules with cornstarch and water evenly according to the mass ratio of 9:0.5:1.5, and use an extruder to puff the mixture. The pulverizer crushes the expanded mixture particles into solid particles with a particle size of 0.45mm;

[0033] 3) Mix the above-mentioned solid particles and culture water in the culture unit according to the volume ratio of 1:1, and then add the bacillus named Dalianpingdao-22 and the preservation number CCTCCM2015216 into the culture unit, so that the bacillus in the culture unit The number of live bacteria is 2×10 10 cfu / L, maintain the dissolved oxygen in the water in the culture unit at 6~8mg / L, and collect bioflocs when the total...

Embodiment 3

[0038] A method for cultivating bioflocs, comprising the steps of:

[0039] 1) Choose corn stalks that have been dried in the sun, crush the stalks with a pulverizer, and then screen them with a 20-mesh sieve to obtain straw particles with a particle size of less than 0.1mm;

[0040] 2) Mix the above-mentioned straw granules with cornstarch and water evenly according to the mass ratio of 11:0.5:1.5, and extrude the mixture with an extrusion extruder, control the temperature of the extrusion cavity of the extrusion extruder at 130°C, and then The pulverizer crushes the expanded mixture particles into solid particles with a particle size of 0.55mm;

[0041] 3) Mix the above-mentioned solid particles and aquaculture water in the culture unit according to the volume ratio of 1.2:1, and then add the bacillus named Dalianpingdao-22 and the preservation number CCTCCM2015216 into the culture unit, so that the bacillus in the culture unit The number of live bacteria is 5×10 10 cfu / L,...

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Abstract

The invention discloses a method for culturing biofloc and a method for applying the biofloc to aquaculture, and belongs to the technical field of aquaculture. Straw is crushed, and evenly mixed with corn starch and water, the mixture is cut up to solid particles of 0.45 mm to 0.55 mm after being puffed, and the solid particles and aquaculture water are mixed according to the volume ratio of (1-1.2):1; bacillus is added to the aquaculture water, so that the viable count is (2-5)*1,010 cfu / L; suspended solids in a culturing unit are collected, and the biofloc is obtained; when aquaculture is carried out, 0.3-0.5 kg of biofloc is added to each stere of culture pond water, the biofloc is sprayed into the aquaculture water, and the volume ratio of the biofloc to water in a culture pond is kept to range from 10% to 15% in the culture period. A carbon source is obtained through biochemical treatment on the straw, the culturing cost of the biofloc is reduced, the biofloc is promoted to fast grow, and the biofloc with the grain size of 500 micrometers to 1,000 micrometers is obtained. The quality of the aquaculture water is adjusted, and the utilization rate of bait is increased.

Description

technical field [0001] The invention relates to a method for cultivating biological flocs and an application method in aquaculture, belonging to the technical field of aquaculture. Background technique [0002] my country is a big aquaculture country. In the current aquaculture activities, farmed fish can only digest and absorb 20%-25% of the protein in the bait, and the rest exists in the aquaculture water environment in the form of ammonia nitrogen, residual bait and feces. The discharge of aquaculture sewage during the breeding process has caused serious pollution to the water environment. Biofloc technology is currently the leading aquaculture water control technology at home and abroad. There are two main ecological functions of biofloc in aquaculture ponds: one is that heterotrophic bacteria in biofloc convert inorganic nitrogen in aquaculture water into bacteria Its own components (bacterial protein) can achieve the effect of reducing harmful substances such as ammoni...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12R1/07
Inventor 包卫洋陈海兵于明超吉红九
Owner YANGZHOU UNIV
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