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A total cholesterol detection reagent with high accuracy and strong anti-interference ability

A technology for total cholesterol and detection reagents, applied in the field of total cholesterol detection reagents, to achieve the effects of improving stability, improving accuracy, and removing interference

Active Publication Date: 2019-10-15
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has good precision and accuracy, and high sensitivity, but it is easily interfered by various interference factors. For example, substances in serum such as hemoglobin, bilirubin, ascorbic acid, etc. will also interfere with the determination results. Based on the invention of a total cholesterol detection reagent with good accuracy and stronger anti-interference ability

Method used

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  • A total cholesterol detection reagent with high accuracy and strong anti-interference ability
  • A total cholesterol detection reagent with high accuracy and strong anti-interference ability
  • A total cholesterol detection reagent with high accuracy and strong anti-interference ability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) A total cholesterol detection reagent with high accuracy and strong anti-interference ability, the composition of the reagent R is as follows:

[0041] Reagent R contains:

[0042] CAPS buffer (pH7.0) 50mmol / L

[0043] 4-aminoantipyrine 1.0mmo1 / L

[0044] Cholesterol Esterase (CHE) 2000U / L

[0045] Cholesterol oxidase (CHO) 2000U / L

[0046] Peroxidase (POD) 2500U / L

[0047] 3,5-Dichloro-2-hydroxybenzene sulfonate (DHBS) 3.5mmol / L

[0048] Magnesium chloride 2.5mmol / L

[0049] Trehalose 20g / L

[0050] Mannitol 20g / L

[0051] Hydroxyethylidene diphosphonic acid 2ml / L

[0052] Octadecyl Trimethyl Ammonium Bromide · 0.3g / L

[0053] EMULGEN-707 0.35ml / L

[0054] Ascorbate oxidase 2.5KU / L,

[0055] Bilirubin Oxidase 2KU / L

[0056] Preservative 0.5ml / L

[0057] 2) Testing method:

[0058] The total cholesterol detection reagent described in this embodiment uses an automatic biochemical analyzer, such as a Hitachi 7180 automatic analyzer, and the like, and the end point method is used for determin...

Embodiment 2

[0063] Correlation experiment: The reagents were prepared using the formula in Example 1, and compared with the total cholesterol kit of a company approved by the State Food and Drug Administration, which is common in the market, and 20 clinical serum samples were tested at the same time. The test results are shown in Table 2. Show. And obtained the correlation curve of the two reagents (such as figure 1 Shown).

[0064] Table 2 Comparison of test results between the reagents of Example 1 and the common and approved total cholesterol determination kits in the market

[0065]

[0066] The test results show that the correlation coefficient of the two kits is 0.9997, indicating that the two have a great correlation. The stabilizer and anti-interference agent added in the reagent of Example 1 did not affect the accuracy of the reagent. It shows that the reagent of Example 1 has good accuracy.

Embodiment 3

[0068] Interference test: Take fresh mixed serum, divide into 2 equal parts, and then divide each equal part into 5 equal parts, add different interfering substances to make the concentration in the serum meet the requirements of Table 3. Then, Example 1 and the control reagent (same as Example 2) were used to compare and determine the total cholesterol content in the serum at the same time. The determination results of each group after adding different interfering substances are shown in Table 3. Relative deviation (%) = (Measurement average value of interfering samples-Measurement average value of samples without interfering substances) / Measurement average value of no interfering substances × 100%.

[0069] Table 3 Comparison of anti-interference performance of reagents in the examples

[0070]

[0071] It can be seen from Table 3 that the reagent of Example 1 did not significantly interfere with the test results when ascorbic acid ≤ 2000 μmol / L, bilirubin ≤ 986 μmol / L, and hemog...

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Abstract

The invention provides a total cholesterol detection reagent with high accuracy and disturbance resisting capacity and relates to the technical field of total cholesterol detection. A biological buffer CAPS (3-(hexanaphthene amino)-1-propanesulfonic acid) is used in the reagent R, and therefore the buffering ability is ensured; a surfactant octadecy trimethyl ammonium bromide and a surfactant EMULGEN-707 are added into the reagent R, the emulsification effect of the reagent is improved well, and the accuracy and specificity of the reagent are improved; stabilizers such as trehalose and mannitol are added into the reagent R, and the stability of the reagent can be improved effectively; hydroxy ethidene diphosphonic acid is added into the reagent R, heavy metal ions can be effectively chelated, and the accuracy of the reagent can be improved; chemical-resistant succinoxidase and bilrubin oxidase are added into the reagent, disturbance of ascorbic acid and bilirubin can be effectively removed, and the reagent is simple in preparation, low in price and very suitable for large-area clinical popularization.

Description

Technical field [0001] The invention relates to the technical field of total cholesterol detection, in particular to a total cholesterol detection reagent with high accuracy and strong anti-interference ability. Background technique [0002] Total cholesterol refers to the sum of cholesterol contained in all lipoproteins in the blood. The total cholesterol level of the population mainly depends on genetic factors and lifestyle. Total cholesterol includes free cholesterol and cholesterol ester. The liver is the main organ for synthesis and storage. Cholesterol is an important raw material for the synthesis of physiologically active substances such as adrenal cortex hormones, sex hormones, bile acids and vitamin D. It is also the main component of cell membranes. Cholesterol (CHO) and cholesterol esters (ChE) are lipids, which are fats present in the blood A form of Cholesterol has many important functions in the body. Its main functions are: cholesterol is an important componen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/28C12Q1/26
Inventor 李静高雪萍谭柏清
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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