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Application of trichoderma pseudokoningii exopolysaccharide in preventing and treating colon cancer and application of chemotherapeutic drug combined with trichoderma pseudokoningii exopolysaccharide in treating colon cancer

A technology for Trichoderma cells and colon cancer, applied in the field of medicine, can solve problems such as no reports on research, and achieve the effects of improving the level of IL-2, prolonging life and improving immunity of the body

Active Publication Date: 2016-03-23
WANNAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the functional research on the exopolysaccharide of Trichoderma pseudokonii has become a hotspot in the research of Trichoderma pseudokonii, but the anti-tumor activity of this polysaccharide, especially colon cancer, has not been reported yet.

Method used

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  • Application of trichoderma pseudokoningii exopolysaccharide in preventing and treating colon cancer and application of chemotherapeutic drug combined with trichoderma pseudokoningii exopolysaccharide in treating colon cancer
  • Application of trichoderma pseudokoningii exopolysaccharide in preventing and treating colon cancer and application of chemotherapeutic drug combined with trichoderma pseudokoningii exopolysaccharide in treating colon cancer
  • Application of trichoderma pseudokoningii exopolysaccharide in preventing and treating colon cancer and application of chemotherapeutic drug combined with trichoderma pseudokoningii exopolysaccharide in treating colon cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Inhibitory effects of exopolysaccharides from Trichoderma pseudokonii for treatment and prophylaxis on tumor growth in colon cancer BALB / C mice

[0042] By inoculating BALB / C mice with the CT26 colon cancer cell line, a colon cancer nude mouse xenograft model was established. The treatment approach was to inoculate the tumor first, and then administer the drugs in groups the next day; to detect the therapeutic effect of EPS on colon cancer tumors

[0043] The way of prevention is to test the effect of EPS on the prevention of colon cancer by adopting the way of intragastric administration first and then inoculating the tumor strain to continue the administration.

[0044] Specific experimental methods:

[0045] One week before the experiment, 18-22g female BALB / C mice were purchased and raised in the animal room (room temperature 23±2°C, humidity 50%±10%), and colon cancer CT26 cells in the logarithmic phase were collected and diluted with phosphate buffered saline (PBS...

Embodiment 2

[0074] Inhibitory effect of EPS combined with 5-fluorouracil on growth of colon cancer transplanted tumor in nude mice

[0075] BALB / C mice were inoculated with colon cancer cell lines to establish a colon cancer mouse transplantation tumor model, and the colon cancer mice were treated by intragastric administration, and the effect of EPS combined with chemotherapy drug 5-FU on colon cancer tumor-bearing mice was observed. role.

[0076] experimental method:

[0077] The colon cancer xenograft tumor mouse model was established, and the method was the same as that in Example 1.

[0078] 45 successfully modeled female BALB / C mice were randomly divided into tumor model group (control group), 5-FU group (20mg / kg / d), EPS low, medium and high dose combined with 5-FU group, respectively. Dosage EPS50mg / kg / d+5-FU20mg / kg / d, EPS100mg / kg / d+5-FU20mg / kg / d, EPS200mg / kg / d+5-FU20mg / kg / d. Oral administration once a day at a fixed time for 10 consecutive days. After the last administration,...

Embodiment 3

[0099] Trichoderma pseudokonii exopolysaccharide prolongs the survival of CT26 colon cancer mice

[0100] By establishing a mouse model of CT26 colon cancer, observe the effect of Trichoderma pseudoconii exopolysaccharide EPS at 100 mg / kg / d and its combination with 5-FU on the survival of CT26 tumor-bearing mice.

[0101] experimental method:

[0102] The colon cancer xenograft tumor mouse model was established, and the method was the same as in Example 1. The mice were randomly divided into 4 groups, 7 in each group, namely the model control group (normal saline), the polysaccharide treatment group (100mg / kg / dEPS), the 5-FU group (20mg / kg / d), and the combined drug group ( 100mg / kg / dEPS+20mg / kg / d5-FU), orally administered. On the second day of inoculation, the administration was given at a fixed time, once a day, for a total of 10 days. After stopping the drug, the mice were observed to die. Observe the mental state, activity, diet, hair, etc. of the mice, and record the s...

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Abstract

The invention discloses application of trichoderma pseudokoningii exopolysaccharide in preventing and treating colon cancer and application of a chemotherapeutic drug combined with the trichoderma pseudokoningii exopolysaccharide in treating the colon cancer. The trichoderma pseudokoningii exopolysaccharide (EPS) can significantly inhibit the growth of tumor masses in a mouse with the colon cancer, and can improve mouse immune organ index and the levels of interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) in mouse serum, increase the proportion of mouse cytotoxic T lymphocytes (CD8+T cells) in total T lymphocyte calculation and significantly activate mouse T lymphocytes and B lymphocytes; therefore, the EPS has an effect on treating the colon cancer; and the EPS, when taken for a preventive purpose, can also effectively improve the antitumor capacity of a body. In addition, the EPS, when combined with an existing chemotherapeutic drug, namely 5-fluorouracil (5-FU), is significantly better than the single medication of the 5-FU in antitumor effect. Moreover, the EPS can improve the life quality of the mouse with the colon cancer and can prolong the service life of the mouse.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of the exopolysaccharide of Trichoderma pseudokonii in the treatment and prevention of colon cancer, and its application in combination with chemotherapy drugs for the treatment of colon cancer. Background technique [0002] Cancer is a well-known serious disease that seriously endangers human life and health and threatens the development of human society. With the increasing pollution of the human living environment in today's society, the increase in people's living pressure, the aging of the population and the changes in people's daily eating habits have led to an increase in cancer mortality. Gastrointestinal tumors are common malignant tumors worldwide, mainly including gastric cancer, esophageal cancer, colorectal cancer, and liver cancer. [0003] In the United States, colorectal cancer ranks third in both cancer incidence and mortality. In 2014, approx...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61K31/715A61P35/00A61K31/513
CPCA61K31/513A61K31/715A61K36/06A61K2300/00
Inventor 陈靠山朱磊陈国创芦静波房芳
Owner WANNAN MEDICAL COLLEGE
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