The application of Trichoderma pseudoconii exopolysaccharide in the treatment and prevention of colon cancer, and its application in combination with chemotherapy drugs in the treatment of colon cancer

A technology of Trichoderma and colon cancer, applied in the field of medicine, can solve the problems that have not yet been reported in research, and achieve the effects of improving the level of IL-2, prolonging life and promoting proliferation

Active Publication Date: 2019-03-12
WANNAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, the functional research on the exopolysaccharide of Trichoderma pseudokonii has become a hotspot in the research of Trichoderma pseudokonii, but the anti-tumor activity of this polysaccharide, especially colon cancer, has not been reported yet.

Method used

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  • The application of Trichoderma pseudoconii exopolysaccharide in the treatment and prevention of colon cancer, and its application in combination with chemotherapy drugs in the treatment of colon cancer
  • The application of Trichoderma pseudoconii exopolysaccharide in the treatment and prevention of colon cancer, and its application in combination with chemotherapy drugs in the treatment of colon cancer
  • The application of Trichoderma pseudoconii exopolysaccharide in the treatment and prevention of colon cancer, and its application in combination with chemotherapy drugs in the treatment of colon cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Treatment and preventive administration of extracellular polysaccharide of Trichoderma kansei on tumor growth of colon cancer BALB / C mice

[0042] By inoculating the CT26 colon cancer cell line in BALB / C mice, the transplanted tumor model of colon cancer was established in nude mice. The treatment method was firstly connected with the tumor, and the next day was administered in groups for testing; the effect of EPS on the treatment of colon cancer tumors was tested.

[0043] The method of prevention was tested by gavage and then inoculation with tumor strains to continue the administration, aiming to detect the effect of EPS on colon cancer tumor prevention.

[0044] Specific experimental methods:

[0045] One week before the experiment, 18-22g female BALB / C mice were purchased and kept in the animal room (room temperature 23±2°C, humidity 50%±10%), and log-phase colon cancer CT26 cells were collected and diluted with phosphate buffered saline (PBS) To 2×10 5 Pieces / mL. Under ...

Embodiment 2

[0074] Inhibitory effect of EPS combined with 5-fluorouracil on the growth of transplanted colon cancer in nude mice

[0075] By inoculating colon cancer cell lines in BALB / C mice, a colon cancer mouse xenograft model was established. The colon cancer mice were treated by gavage method. The effect of EPS combined with chemotherapy drug 5-FU on colon cancer tumor-bearing mice was observed. The role of.

[0076] experimental method:

[0077] To establish a colon cancer xenograft mouse model, the method is the same as that of case 1.

[0078] 45 successfully modeled female BALB / C mice were randomly divided into tumor model group (control group), 5-FU group (20mg / kg / d), low, medium, and high-dose EPS combined with 5-FU groups, respectively. Dosage: EPS 50mg / kg / d+5-FU 20mg / kg / d, EPS 100mg / kg / d+5-FU20mg / kg / d, EPS 200mg / kg / d+5-FU 20mg / kg / d. Gavage is given once a day at a fixed time for 10 consecutive days. After the last administration, the patients were fasted for 12 hours, weighed, and...

Embodiment 3

[0099] Exopolysaccharide from Trichoderma kansei to prolong the survival time of CT26 colon cancer mice

[0100] By establishing a CT26 colon cancer mouse animal model, observe the effect of Trichoderma pseudocondini exopolysaccharide EPS at a dosage of 100mg / kg / d and its combination with 5-FU on the survival of CT26 tumor-bearing mice.

[0101] experimental method:

[0102] A mouse model of colon cancer transplantation tumor was established, and the method was the same as in Example 1. The mice were randomly divided into 4 groups, 7 in each group, namely the model control group (normal saline), the polysaccharide treatment group (100mg / kg / dEPS), the 5-FU group (20mg / kg / d), and the combination medication group ( 100mg / kg / d EPS+20mg / kg / d5-FU), intragastric administration. On the second day of vaccination, the drug was administered at a fixed time, once a day for 10 days. The mice were observed to die after stopping the drug. Observe the mice's mental state, activity, diet, hair, e...

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Abstract

The invention discloses application of trichoderma pseudokoningii exopolysaccharide in preventing and treating colon cancer and application of a chemotherapeutic drug combined with the trichoderma pseudokoningii exopolysaccharide in treating the colon cancer. The trichoderma pseudokoningii exopolysaccharide (EPS) can significantly inhibit the growth of tumor masses in a mouse with the colon cancer, and can improve mouse immune organ index and the levels of interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) in mouse serum, increase the proportion of mouse cytotoxic T lymphocytes (CD8+T cells) in total T lymphocyte calculation and significantly activate mouse T lymphocytes and B lymphocytes; therefore, the EPS has an effect on treating the colon cancer; and the EPS, when taken for a preventive purpose, can also effectively improve the antitumor capacity of a body. In addition, the EPS, when combined with an existing chemotherapeutic drug, namely 5-fluorouracil (5-FU), is significantly better than the single medication of the 5-FU in antitumor effect. Moreover, the EPS can improve the life quality of the mouse with the colon cancer and can prolong the service life of the mouse.

Description

Technical field [0001] The present invention belongs to the field of medicine, and specifically relates to the application of Trichoderma pseudocone extracellular polysaccharide in the treatment and prevention of colon cancer, and the application of combined chemotherapy drugs to treat colon cancer. Background technique [0002] Cancer is a well-known serious disease that seriously endangers human life and health and threatens the development of human society. With the increasing pollution of the human living environment in today's society, the increasing pressure on people's lives, the aging of the population and the changes in people's daily dietary habits have led to an increase in cancer mortality. Gastrointestinal tumors are common malignant tumors worldwide, including gastric cancer, esophageal cancer, colorectal cancer and liver cancer. [0003] In the United States, colorectal cancer ranks third in both cancer incidence and mortality. In 2014, approximately 71,830 men and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/06A61P35/00A61K31/513
CPCA61K31/513A61K31/715A61K36/06A61K2300/00
Inventor 陈靠山朱磊陈国创芦静波房芳
Owner WANNAN MEDICAL COLLEGE
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