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Tumor-targeted multifunctional nano probe with MRI (Magnetic Resonance Imaging)/SPECT (Single Photon Emission Computed Tomography) bimodal images as well as preparation method and application thereof

A nano-probe and tumor-targeting technology, which can be used in preparations for in vivo experiments, radioactive carriers, emulsion delivery, etc., can solve the problems of complex reaction steps and high prices of precursor raw materials, and achieve cheap and saturated raw material sources. The effect of high magnetization and controllable shape

Inactive Publication Date: 2016-03-23
SHANGHAI NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

The carrier NIRF-CCPM of this patent uses two precursors poly-(PEG-acrylic acid)-b-poly-(triethoxysilyl acrylate) and NIRF dye 3-(triethoxysilyl)propyl-Cy7 to aggregate polymer glue In the method of beam, the reaction steps are complicated and the price of precursor raw materials is high

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  • Tumor-targeted multifunctional nano probe with MRI (Magnetic Resonance Imaging)/SPECT (Single Photon Emission Computed Tomography) bimodal images as well as preparation method and application thereof
  • Tumor-targeted multifunctional nano probe with MRI (Magnetic Resonance Imaging)/SPECT (Single Photon Emission Computed Tomography) bimodal images as well as preparation method and application thereof
  • Tumor-targeted multifunctional nano probe with MRI (Magnetic Resonance Imaging)/SPECT (Single Photon Emission Computed Tomography) bimodal images as well as preparation method and application thereof

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Embodiment 1

[0038] A tumor-targeted multifunctional nanoprobe with MRI / SPECT dual-modal imaging, including FeFe 3 o 4 Nanoparticles (MNPs), coupled on FeFe 3 o 4 The liposome DSPE-PEG-RGD on the surface layer of the nanoparticles and the MNPs-DSPE-PEG-RGD nanoprobe composed of the liposome DSPE-PEG, the diameter of the MNPs-DSPE-PEG-RGD nanoprobe is 12-13nm.

[0039] The preparation method of the above-mentioned tumor-targeted multifunctional nanoprobe with MRI / SPECT dual-modal imaging comprises the following steps: take 5mgDSPE-PEG-RGD and 10mgDSPE-PEG dissolved in 2mL of chloroform, add 0.5mL of 1mg / mL FeFe 3 o 4 Nanoparticle solution Chloroform solution, shake the bed overnight at room temperature, remove chloroform by rotary evaporation at room temperature, then rotary steam in a water bath at 80°C for 1min, then add 5mL of water, dialyze with a 3500Da dialysis bag for two days, and filter with a 0.22μm filter membrane to obtain 1mg / mL The nanoprobe MNPs-DSPE-PEG-RGD aqueous solu...

Embodiment 2

[0041] A tumor-targeted multifunctional nanoprobe with MRI / SPECT dual-modal imaging, including FeFe 3 o 4 Nanoparticles (MNPs), coupled on FeFe 3 o 4 The liposome DSPE-PEG-RGD on the surface layer of the nanoparticles and the MNPs-DSPE-PEG-RGD nanoprobe composed of the liposome DSPE-PEG, the diameter of the MNPs-DSPE-PEG-RGD nanoprobe is 12-13nm.

[0042] The preparation method of the above-mentioned tumor-targeted multifunctional nanoprobe with MRI / SPECT dual-modality imaging comprises the following steps: take 4mgDSPE-PEG-RGD and 7mgDSPE-PEG dissolved in 2mL chloroform, add 0.5mL concentration of 0.5mg / mL FeFe 3 o 4 Nanoparticle solution chloroform solution, shake the bed overnight at room temperature, remove chloroform by rotary evaporation at room temperature, then add 3mL water, dialyze with a 3500Da dialysis bag for two days, and filter with a 0.22μm filter membrane to obtain 1mg / mL nanoprobe MNPs-DSPE-PEG - RGD aqueous solution, stored in a 4°C refrigerator for lat...

Embodiment 3

[0044] A tumor-targeted multifunctional nanoprobe with MRI / SPECT dual-modal imaging, including FeFe 3 o 4 Nanoparticles (MNPs), coupled on FeFe 3 o 4 The liposome DSPE-PEG-RGD on the surface layer of the nanoparticles and the MNPs-DSPE-PEG-RGD nanoprobe composed of the liposome DSPE-PEG, the diameter of the MNPs-DSPE-PEG-RGD nanoprobe is 12-13nm.

[0045] The preparation method of the above-mentioned tumor-targeted multifunctional nanoprobe with MRI / SPECT dual-modality imaging comprises the following steps: take 6mgDSPE-PEG-RGD and 13mgDSPE-PEG dissolved in 2mL chloroform, add 0.5mL concentration of 1.5mg / mL FeFe 3 o 4 Nanoparticle solution chloroform solution, shake the bed at room temperature overnight, remove chloroform by rotary evaporation at room temperature, then add 7mL water, dialyze with 3500Da dialysis bag for two days, and filter with 0.22μm filter membrane to obtain 1mg / mL nanoprobe MNPs-DSPE-PEG - RGD aqueous solution, stored in a 4°C refrigerator for later ...

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Abstract

The invention relates to a tumor-targeted multifunctional nano probe with MRI (Magnetic Resonance Imaging) / SPECT (Single Photon Emission Computed Tomography) bimodal images as well as a preparation method and application thereof. The nano probe comprises an MNPs-DSPE-PEG-RGD nano probe composed of Fe@Fe3O4 nano particles and liposome DSPE-PEG-RGD and liposome DSPE-PEG which are coupled on the surface layer of the Fe@Fe3O4 nano particles, and radionuclide I125 can also be marked on the MNPs-DSPE-PEG-RGD nano probe to form an MNPs-DSPE-PEG-RGD-I125 nano probe. The preparation method comprises the following steps: dissolving DSPE-PEG-RGD and DSPE-PEG in an organic solvent, adding an Fe@Fe3O4 nano particle solution, and mixing uniformly to obtain the MNPs-DSPE-PEG-RGD nano probe; and marking with I125 by a chloramine-T method to obtain the MNPs-DSPE-PEG-RGD-I125 nano probe. The prepared MNPs-DSPE-PEG-RGD nano probe is used for MRI of human brain glioma, and the prepared MNPs-DSPE-PEG-RGD-I125 nano probe is used for SPECT. Compared with the prior art, the probe provided by the invention has the advantages of strong targeting property and the like, and multiple molecular imaging probes can be achieved on the same molecule.

Description

technical field [0001] The invention relates to a multifunctional nanoprobe, in particular to a tumor-targeting multifunctional nanoprobe with MRI / SPECT dual-mode imaging, and its preparation and application. Background technique [0002] Molecular imaging (MI) was first proposed by Weissleder of Harvard University. The technologies currently used in molecular imaging research include CT, MRI, US, PET, SPECT and optical imaging. However, no single modality imaging can provide all the necessary information for disease diagnosis and treatment. In view of the advantages and disadvantages of each single modality imaging, in recent years, dual (multi) modality molecular imaging has gradually become a research and development hotspot, and it has also driven the development and construction of dual (multi) modality molecular probes. Nanotechnology provides a platform for the construction of ideal dual (multi)modal molecular probes, and the research on dual-modal probes integrating...

Claims

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Application Information

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IPC IPC(8): A61K49/14A61K49/12A61K49/18A61K51/08A61K51/06A61K51/12A61K101/02
Inventor 周治国王晶赵恒周萍杨仕平
Owner SHANGHAI NORMAL UNIVERSITY
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