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A kind of tea saponin extract and preparation method thereof

A technology of tea saponins and triterpene saponins, applied in the preparation of anti-inflammatory and blood lipid-lowering health food or medicine, in the field of triterpene saponins, can solve the problems of hindering the popularization and application of extracts, difficult quality control, complex composition, etc. problems, to achieve the effects of small drug dosage, improved physical and chemical properties, and clear composition

Active Publication Date: 2018-10-12
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, since the natural tea saponin contains a variety of substituted ester groups, its water solubility is weak, which hinders the further application of the extract; the method of physically and chemically modifying tea saponin is the only way to expand its application range. One of the research hotspots [Song Binglei, Preparation and properties of deesterified tea saponin, Biomass Chemical Engineering, 2011, 45(5), 8-10; Qian Wei, Research progress in the preparation and application of modified tea saponin derivatives, Economic Forest Research, 2012, 30(4), 168-173]
[0004] Aiming at the defects that the existing tea saponin products have complex composition and difficult quality control, the inventor designed and adopted alkaline hydrolysis method to selectively remove the ester group in tea saponin according to its structural characteristics, and combined with macroporous resin, reversed-phase silica gel and other column layers Analysis and preparation technology, in order to obtain a new product of tea saponin with stable chemical composition and controllable quality

Method used

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  • A kind of tea saponin extract and preparation method thereof
  • A kind of tea saponin extract and preparation method thereof
  • A kind of tea saponin extract and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: the preparation of camellia oleifera saponin

[0025] The cake obtained after oil-pressing camellia seed (Camellia oleifera, batch 20120901, collected from Guangnan, Yunnan) was selected.

[0026](1) Weigh 800 g of Camellia oleifera seed cake, crush it, add 10 times the amount of 80% ethanol to soak overnight, reflux extraction twice, each time for 2 hours, and concentrate under reduced pressure to about 3 L. 2L of the concentrated solution was taken after being adsorbed by an AB-8 macroporous adsorption resin column (15×60cm, column volume 2L), followed by water (8L), 10% ethanol (8L), 30% ethanol (8L), 75% ethanol ( 8 L) elution, the 75% ethanol elution part was concentrated under reduced pressure, and dried to obtain 40.9 g of Camellia oleifera Total Saponins CSA.

[0027] (2) Take 10g of CS-A, add to 100ml 2mol / L NaOH solution, hydrolyze at 95°C for 5h, neutralize with 5mol / L HCl to pH4-6, apply AB-8 macroporous adsorption resin (column volume 400ml),...

Embodiment 2

[0038] Embodiment 2: the preparation of camellia oleifera saponin

[0039] The root of camellia oleifera (batch 20110901, collected from Qichun County, Hubei Province) was selected and prepared with reference to Example 1.

[0040] (1) Weigh 100g of Camellia oleifera root, add 10 times the amount of 60% ethanol to soak overnight after crushing, reflux extraction twice, each time for 1.5h, concentrate under reduced pressure to 500mL, put on AB-8 macroporous adsorption resin column (column volume 300mL ) adsorption, followed by water (5L), 30% ethanol (1L), 60% ethanol (1L) elution, 60% ethanol elution part concentrated under reduced pressure, drying, to get Camellia oleifera total saponins CSA21.4g.

[0041] (2) Take 10g of CSA, add it to 100ml 2mol / L NaOH solution, hydrolyze it at 95°C for 5h, neutralize it with 5mol / L HCl to pH4-6, put it on AB-8 macroporous adsorption resin (column volume 400ml), and then water ( 1.6L), 75% ethanol (1.6L), the collected 75% ethanol eluate w...

Embodiment 3

[0044] Embodiment 3: the preparation of tea seed saponin

[0045] Tea (Camellia sinensis, batch 20131001, collected from Guangnan, Yunnan) seeds were selected and prepared with reference to Example 1.

[0046] (1) Weigh 100g of tea seeds, add 10 times the amount of 60% ethanol to soak overnight after crushing, reflux extraction twice, each time for 1.5h, concentrate under reduced pressure to 500mL, put on AB-8 macroporous adsorption resin column (column volume 300mL ) adsorption, followed by water (5L), 30% ethanol (1L), 60% ethanol (1L) elution, 60% ethanol eluted part concentrated under reduced pressure, dried to obtain total tea saponins CSA27g.

[0047] (2) Take 10g of CSA, add it to 100ml 2mol / L NaOH solution, hydrolyze it at 95°C for 5h, neutralize it to pH4-6 with 5mol / L HCl, put it on AB-8 macroporous adsorption resin (column volume 400ml), and then water ( 1.6L), 75% ethanol (1.6L), the collected 75% ethanol eluate was concentrated under reduced pressure and dried to...

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Abstract

The invention discloses a triterpenoid saponin compound shown by a formula (I) and a preparation method thereof. The triterpenoid saponin compound is prepared by the following main steps: selecting a camellia plant raw material, performing extraction by adopting water or an alcohol-water mixed solvent, and performing macroporous resin column enrichment to obtain a theasaponin extract CSA; performing hydrolytic reaction on CSA in an alkali solution of which the pH value is 10-13, performing acid neutralization, and then performing macroporous resin column refining to obtain a theasaponin extract CSB; and separating CSB by adopting a reverse phase silica gel column chromatography technology to obtain the triterpenoid saponin compound and a composition thereof. The invention simultaneously discloses an application of the theasaponin extract in preparation of medicines and health foods, and is characterized in that the theasaponin extract has pharmacological actions of anti-inflammation, anti-hyperlipidemia and the like. The theasaponin provided by the invention is clear and definite in active ingredient, stable and controllable in quality and concise in preparation process, is easy for industrial amplification, and has a good application value.

Description

technical field [0001] The invention relates to a triterpene saponin compound extracted from Camellia plants, and also relates to the application of the triterpene saponin compound in the preparation of anti-inflammatory and blood lipid-lowering health food or medicine, belonging to the field of biomedicine. technical background [0002] Camellia plants of the family Theaceae are widely distributed in the hilly areas of southern China and other Southeast Asian countries, and have been planted in China for more than 2,000 years. Tea is a famous specialty in China. In addition, oil can be extracted from the seeds of Camellia plants, and the unsaturated fatty acid content of the obtained tea oil is as high as 90%, which has high nutritional value; the unique tea saponin content in the tea seed cake extracted from the camellia oil is about 10%. A variety of tea saponins have also been found in the seeds, roots, flower buds and leaves of Camellia plants. These tea saponins have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P29/00A61P3/06A23L33/11
Inventor 刘江云王增尚陆昊许琼明郝丽莉
Owner SUZHOU UNIV