Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria

A technology of mecitrione and degrading bacteria, which is applied in the field of biotechnology, can solve the problems of ecological system hazards, the safety production of agricultural products that affect the health, and excessive pesticide residues, so as to protect the ecological environment, solve the problem of excessive pesticide residues of mecitrione, The effect of low cost of production and use

Inactive Publication Date: 2016-03-23
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system.
The extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health and the safe production of agricultural products

Method used

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  • Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria
  • Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Isolation and identification of bacterial strains

[0021] Collect the soil polluted by metrizone for a long time, take 15g soil sample and place in 100mL inorganic salt liquid medium (NH 4 NO 3 1.0 g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, MgSO 4 0.2 grams, 1.0 grams of NaCl, 1000 milliliters of water, pH7.0-7.2), 30 ℃, 150r / min cultivated for 7 days, transferred to the same inorganic Saline liquid medium, successively transferred 4 times. The degradation effect was determined by liquid chromatography. Take 0.5 mL of the effective enrichment solution, and serially dilute, and apply 100 μL of each gradient diluted enrichment solution on an inorganic salt solid medium plate containing 50 mg / L mecitrione pesticide, and incubate at 30° C. for 5 days. Pick a single colony grown on the plate and inoculate it in a test tube of liquid LB medium (1g of peptone, 0.5g of yeast extract, 0.5g of NaCl, 20g of agar powder, 1000ml of water, pH7.0-7.2) at 30°C , 160r / ...

Embodiment 2

[0023] The degradative effect of embodiment 2 bacterial strains to mecitrione

[0024] Degradation characteristics of mecitrione by degrading bacteria QCT10 in inorganic salt liquid medium: Pick a single colony of QCT10 in 50ml LB liquid medium, shake and culture at 30°C and 160r / min for 12h to obtain fresh bacterial liquid. Centrifuge 3ml of the cultured fresh bacterial solution at 6000r / min for 5min, discard the supernatant, add 3ml of sterile water to resuspend, and obtain the bacterial solution after centrifugation and suspension. In 20ml of GMSM liquid culture medium (3.0 grams of glucose, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5g, NaCl1.0g, MgSO 4 ·7H 2 (00.2 gram, 1000 milliliters of water, pH7.0-7.2), insert the bacterium liquid after the centrifugal suspension of 1.5ml, 30 ℃, 150r / min cultivates, and the content of sampling determination metrizone in the 7th day. The results are shown in Table 1. The degradation rate of mecitrione by the degrading bacteria QCT10 reached 7...

Embodiment 3

[0028] The preparation of embodiment 3 bacterial agents and the degradation effect of metrizone

[0029] The original seed of the pesticide residue degrading bacteria QCT10 of the present invention was activated on the inclined surface of the LB test tube. Inoculate the bacteria on the slant of the test tube into a 1000ml shaker flask containing 200ml of broth medium (10g of beef extract, 5g of peptone, 5g of NaCl, 1000ml of water, pH7.0-7.2), and cultivate it at a constant temperature of 30°C until logarithmic period, ready to inoculate the seed pots. 500 liters of seed tanks, 400 liters of feeding capacity, medium formula (mass percentage): glucose 1.0wt%, NH 4 NO 3 1.0wt%, K 2 HPO 4 0.2wt%, MgSO 4 0.05wt%, NaCl0.01wt%, CaCO 30.3%, yeast extract 0.02%, pH 7.2-7.5. 121 DEG C of high-pressure damp-heat sterilization after feeding feed is finished, and after sterilization completes and cools to 33 DEG C, inoculate into 500 liters of seed jars by the inoculum amount of ab...

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Abstract

The invention provides metribuzin pesticide residue degrading bacteria and a microbial agent produced through the same. The bacterial strain is gram-positive bacteria, and is authenticated as Rhodococcus sp. and preserved in the China Center for Type Culture Collection on June 29, 2015, and the preservation number of the bacterial strain is CCTCC NO: M2015392. A culture solution obtained after QCT10 bacterial strain fermentation is the microbial agent. According to the QCT10 bacterial strain and the microbial agent produced through the same, the quantity of metribuzin pesticide residues in soil can be reduced by 50% or above, and the problem that the metribuzin pesticide residues exceed the standard in agricultural production can be solved.

Description

technical field [0001] The invention belongs to the high-tech field of biology, and provides a metrizone pesticide residue-degrading bacterium and its production bacterium and application. Background technique [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by plant diseases, insects, and weed damage for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an invaluable role in the modernization process. But at the same time, pesticides are some toxic and harmful chemical substances. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system. Extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health and the safe production of ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C12N1/205C12R2001/01
Inventor 黄星宋嫚郭素会成明根张浩孙斌李强
Owner NANJING AGRICULTURAL UNIVERSITY
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