Method for extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen protein simultaneously

A technology of bone collagen and chondroitin, applied in chemical instruments and methods, animal/human proteins, connective tissue peptides, etc., can solve problems such as environmental pollution and waste of resources

Inactive Publication Date: 2016-03-23
WEIHAI RENSHENG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for simultaneously extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen, so as to s

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  • Method for extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen protein simultaneously

Examples

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Embodiment 1

[0030] The method for simultaneously extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen provided by the embodiments of the present invention comprises the following steps:

[0031] S101: Wash the Spanish mackerel bones and add them to water, heat them to boil, filter, and filter the residue for later use;

[0032] S102: Add 10% citric acid to the filter residue, soak and filter, set aside the filtrate, and dry the filter residue to obtain mackerel bone calcium powder;

[0033] S103: adding protease to the filtrate in step S102 for enzymolysis to obtain an enzymolysis solution;

[0034] S104: adjusting the pH value of the enzymolysis solution to 7 with an alkaline solution, adding ethanol to precipitate Spanish mackerel chondroitin, and using the remaining liquid for later use;

[0035] S105: Distilling the remaining liquid in step S104 under reduced pressure to recover ethanol, and salting out the remaining material to obtain Spanish mackerel collagen;...

Embodiment 2

[0038] The method for simultaneously extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen provided by the embodiments of the present invention comprises the following steps:

[0039] S201: After the mackerel bones are cleaned, put the mackerel bones into water, and the quality of the water is 0.5 times the mass of the mackerel bones, heat and boil, filter, and filter the residue for later use;

[0040] S202: Add 10% citric acid to the filter residue, soak and filter, set aside the filtrate, and dry the filter residue to obtain mackerel bone calcium powder;

[0041] S203: adding protease to the filtrate in step S202 for enzymolysis to obtain an enzymolysis solution;

[0042] S204: adjusting the pH value of the enzymolysis solution to 8 with an alkaline solution, adding ethanol to precipitate Spanish mackerel chondroitin, and using the remaining liquid for later use;

[0043] S205: Distilling the remaining liquid in step S204 under reduced pressure to reco...

Embodiment 3

[0046] The method for simultaneously extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen provided by the embodiments of the present invention comprises the following steps:

[0047] S301: After the mackerel bones are cleaned, put the mackerel bones into water, and the quality of the water is 3 times the mass of the mackerel bones, heat with microwave at medium heat, the heating temperature is 85°C, and the heating time is 0.5 hours, filter, filter residue for use;

[0048] S302: Add 10% citric acid to the filter residue, soak and filter, set aside the filtrate, and dry the filter residue to obtain mackerel bone calcium powder;

[0049] S303: adding protease to the filtrate in step S302 for enzymolysis to obtain an enzymolysis solution;

[0050]S304: adjusting the pH of the enzymolysis solution to 7-8 with an alkaline solution, adding ethanol to precipitate Spanish mackerel chondroitin, and using the remaining liquid for later use;

[0051] S305: Distil...

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Abstract

The invention provides a method for extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen protein from marine Spanish mackerel bones simultaneously. When the Spanish mackerel chondroitin and the Spanish mackerel bone collagen protein are extracted, microwave medium baking temperature heat treatment is utilized, citric acid soaking demineralization efficiency can be improved, and accordingly a better effect can be achieved when protease is utilized to conduct enzymolysis. By means of the extraction method, high-purity Spanish mackerel chondroitin powder and Spanish mackerel bone collagen protein powder can be obtained, Spanish mackerel bone oil and Spanish mackerel calcium powder can be further produced in an auxiliary mode, comprehensive utilization of the Spanish mackerel bones is achieved, the method is scientific and environmentally friendly, and meanwhile the problem of chondroitin pyrogenic decomposition is avoided.

Description

technical field [0001] The invention relates to the technical field of biological substance extraction, and more specifically relates to a method for simultaneously extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen. Background technique [0002] Spanish Spanish mackerel, also known as blue-spotted mackerel, belongs to the bony fish class and is a fish species that cannot be farmed. Mackerel contains a variety of biologically active substances, and these biologically active substances have the effects of refreshing, anti-aging, invigorating qi, and calming coughs. They have certain effects on anemia, coughing, premature aging, malnutrition, postpartum weakness, and neurasthenia. adjuvant effect. [0003] Mackerel not only contains a variety of biologically active substances in its body, but also has a variety of beneficial substances in its bones. Chondroitin in fish bones has the functions of inhibiting tumors, improving immunity, anti-mutation, im...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06C07K14/78
Inventor 苗其壮关键
Owner WEIHAI RENSHENG PHARMA GRP
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