Preparation method of silk screen printing sensor for detecting tetracycline and oxytetracycline at same time

A technology of screen printing and screen printing electrodes, which is applied in the field of preparation of screen printing sensors, to achieve the effect of increasing the detection amount, increasing the load points, and ensuring the accuracy

Active Publication Date: 2016-03-23
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many detection methods for antibiotics, but most of them require large-scale equipment and professional operators

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method of screen printing sensor for simultaneous detection of tetracycline and oxytetracycline

[0030] (1) Add 3 μL of hydrochloric acid with a mass fraction of 0.015% dropwise on the working electrode of the dual-channel screen-printed electrode for 1 min, and wash it with ultrapure water;

[0031] (2) Add 3 μL, 0.1 mg / mL zinc oxide nanorod solution dropwise, and dry at room temperature;

[0032] (3) Add 3 μL, 0.1mol / L EDC / NHS solution dropwise, incubate at room temperature for 0.2h, wash with ultrapure water, and dry at room temperature;

[0033] (4) Add 3 μL, 0.1 μmol / L carboxyl- and ferrocene-modified tetracycline aptamer and 3 μL, 0.1 μmol / L carboxyl- and ferrocene-modified oxytetracycline aptamer to dual-channel screen printing, respectively. On the two working electrodes of the electrode, incubate at room temperature for 0.2h, wash with ultrapure water, and dry at room temperature;

[0034] (5) Add a series of tetracycline TET and oxytetracyclin...

Embodiment 2

[0036] A preparation method of screen printing sensor for simultaneous detection of tetracycline and oxytetracycline

[0037] (1) Add 5 μL of hydrochloric acid with a mass fraction of 0.08% dropwise on the working electrode of the dual-channel screen-printed electrode for 10 minutes, and wash it with ultrapure water;

[0038] (2) Add 5 μL, 0.5 mg / mL zinc oxide nanorod solution dropwise, and dry at room temperature;

[0039] (3) Add 5 μL, 0.2mol / L EDC / NHS solution dropwise, incubate at room temperature for 0.8h, wash with ultrapure water, and dry at room temperature;

[0040] (4) Add 5 μL, 6 μmol / L of carboxyl- and ferrocene-modified tetracycline aptamer and 5 μL, 5 μmol / L carboxyl- and ferrocene-modified oxytetracycline aptamer to the dual-channel screen-printed electrode, respectively. On two working electrodes, incubate at room temperature for 0.7h, wash with ultrapure water, and dry at room temperature;

[0041] (5) Add a series of tetracycline TET and oxytetracycline OTC...

Embodiment 3

[0043] A preparation method of screen printing sensor for simultaneous detection of tetracycline and oxytetracycline

[0044] (1) Add 6 μL of hydrochloric acid with a mass fraction of 0.15% dropwise on the working electrode of the dual-channel screen-printed electrode for 30 minutes, and wash it with ultrapure water;

[0045] (2) Add 6 μL, 1.5 mg / mL zinc oxide nanorod solution dropwise, and dry at room temperature;

[0046] (3) Add 6 μL, 0.4mol / L EDC / NHS solution dropwise, incubate at room temperature for 2 hours, wash with ultrapure water, and dry at room temperature;

[0047] (4) Add 6 μL, 10 μmol / L carboxyl- and ferrocene-modified tetracycline aptamer and 6 μL, 10 μmol / L carboxyl- and ferrocene-modified oxytetracycline aptamer to the double-channel screen-printed electrode, respectively. On two working electrodes, incubate at room temperature for 2 hours, wash with ultrapure water, and dry at room temperature;

[0048] (5) Add a series of tetracycline TET and oxytetracycl...

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PUM

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Abstract

The invention relates to a preparation method of a silk screen printing sensor for detecting tetracycline and oxytetracycline at the same time, and belongs to the technical field of novel biological sensor detection. On the basis of the advantages that a silk screen printing electrode is high in conductivity, low in price, high in repeatability, convenient to carry, capable of implementing online detection and the like, the method can adapt to tests in different environments, the uncertain factors caused in the glassy carbon electrode grinding process are reduced, and therefore the good effect on detecting tetracycline and oxytetracycline can be achieved on the basis of the silk screen printing strategy.

Description

technical field [0001] The invention relates to a preparation method of a screen printing sensor for simultaneously detecting tetracycline and oxytetracycline, and belongs to the technical field of novel biosensing detection. Using the detection method of screen-printed electrodes, the difference in signal intensity generated by the change of the distance between ferrocene at the end of the aptamer chain and the electrode before and after tetracycline and oxytetracycline binds to the corresponding aptamer chain is used to determine the difference in the test sample. The content of tetracycline and oxytetracycline, so as to realize the sensitive detection of two tetracycline antibiotics. Background technique [0002] Antibiotics are a class of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher animals and plants in the course of life, which have anti-pathogen or other activities, and can interfere with the development of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 魏琴王琦任祥张勇庞雪辉马洪敏吴丹王超
Owner UNIV OF JINAN
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