44 results about "Oxytetracyclinum" patented technology

The invention provides a preparation method of doxycycline hydrochloride. The preparation method of the present invention comprises: 11a-chloro-6-methine oxytetracycline p-benzenemethanesulfonate hydrogenation reaction under catalyst and poison conditions; then, react with sulfosalicylic acid to obtain α-6-deoxyoxytetracycline Sulfosalicylate; after reacting with hydrogen chloride-ethanol solution, add water to stir and crystallize to obtain doxycycline hydrochloride; wherein, the poison includes: a mixture of sulfur-containing and nitrogen-containing organic compounds. The method of the invention can improve the doxycycline hydrochloride yield and stereoselectivity, and obtain high-quality raw materials.

The invention provides an aptamer electrode for oxytetracycline detection, which is characterized in that the glassy carbon electrode is sequentially decorated with graphene-gold nanocomposites and BSA-OTC complexes from bottom to top. The signal layer is a nucleic acid aptamer modified by methylene blue and folded into a G quadruple structure under the RCA chain amplification reaction. The content of the target is reflected by the amount of methylene blue, and its preparation method is provided at the same time. The preparation method of the present invention is simple and has high performance. It is stable and has good electrode repeatability, and is suitable for the detection of oxytetracycline in drug safety and the practical application of biosensor industrialization.

The invention belongs to the technical field of environmental monitoring, and relates to an oxytetracycline fluorescence detection method based on surface passivation and covalent coupling. The covalent coupling between the surface of two-dimensional MOF nanosheets and DNA can effectively utilize the limited adsorption sites on the surface of MOF nanosheets, enhance the ability of nanosheets to adsorb DNA molecules, and reduce the background fluorescence signal. At the same time, the use of short-chain DNA molecules as signal reporter molecules can effectively improve the fluorescence recovery efficiency, while the addition of surface passivator sodium polystyrene sulfonate in the reaction system further weakens the adsorption ability of nanosheets to signal reporter molecules, making MOF nanosheets The chip sensor improves the linear response to low concentration OTC and enhances the detection performance of OTC. The detection method of the invention can effectively reduce the fluorescence background value, increase the signal response value induced by the target, and effectively improve the detection performance of the fluorescent sensing platform constructed based on the principle of physical adsorption for oxytetracycline.

The invention discloses a method for regulating and controlling compound heavy metal forms of municipal refuse compost by adopting OTC (oxytetracycline). According to the method, a PVC (polyvinyl chloride) pipe, of which the diameter is equal to 3cm, the height is equal to 25cm, is adopted, and 160 g of compost is added into the pipe; 40ml of aqueous solution with the mass concentration of 10 to 20 mmol / kg is added into the compost, wherein the temperature is 19 to 27 DEG C, and the relative humidity is 60% to 72% during the test, and water is supplemented to the compost every day under a natural light condition. A weighing method is adopted for adjusting water content, so as to keep the constant of the water content and make the water content in compost substrate reach to the maximum water-holding capacity of 70%, after curing for 45 days, sampling is carried out to analyze the forms of heavy metal. Experimental results show that after adding the OTC, the ratios of the exchangeable forms of Cd, Cr, Cu, Pb and Zn to the bound form of carbonate are all remarkably increased. By adding the OTC, the bio-available forms of the heavy metal in the compost are increased, and the non-bio-available forms are reduced, and activation is realized for the heavy metal in the compost.