43 results about "Oxytetracyclinum" patented technology

The invention provides preparation of a boron nitride material anchored cobalt ferrite composite catalyst and application of the boron nitride material anchored cobalt ferrite composite catalyst in catalytic degradation of oxytetracycline. According to the preparation method, the boron nitride material anchored cobalt ferrite composite catalyst BNMTs (at) CoFe2O4 and H-BN (at) CoFe2O4 are prepared by adopting a coprecipitation and hydrothermal method. According to an advanced oxidation process based on activated peroxymonosulfate (PMS), the composite catalyst is used for degrading oxytetracycline, and H-BN (at) CoFe2O4 / PMS and BNMTs (at) CoFe2O4 / PMS systems both show good degradation performance and cycling stability on the oxytetracycline. The BNMTs-coated CoFe2O4 has abundant mesopores and oxygen-containing groups, so that CoFe2O4 is more effectively fixed and dispersed, the activation of PMS is remarkably enhanced, and the degradation performance on oxytetracycline is more excellent. The method is expected to provide more thoughts for reasonable design of a boron nitride material and a transition metal activated PMS system and application in the aspects of removal of refractory organic wastewater and the like.

The invention provides a fluorescent palladium nano-cluster and a synthesis method and application thereof. The palladium nano-cluster is obtained through the following preparation method which comprises the steps that with palladium nitrate as a raw material, polyethylenimine as a stabilizer, and ascorbic acid as a reducing agent, through a chemical reduction method, the fluorescent palladium nano-cluster is prepared at one step. The palladium nano-cluster has excellent fluorescence emission performance in an aqueous solution and good light-resistant bleaching performance and stability, and the solution is irradiated under a 365nm ultraviolet lamp to emit bright blue fluorescence. The invention further provides a method for detecting oxytetracycline by adopting the palladium nano-cluster.The oxytetracycline is detected through a fluorescence analysis method, the fluorescence intensity of the palladium nano-cluster is gradually lowered with increase of the concentration of the oxytetracycline, and the method has good selectivity. The detection method is easy to operate, capable of achieving high detection speed and greatly shortening the detection time, good in selectivity and lowin cost.

The invention discloses an oxytetracycline wastewater treatment process. The oxytetracycline wastewater treatment process includes steps that oxytetracycline wastewater is filtered by a centrifugal machine and subjected to adsorption through adsorption resins, the potential of hydrogen (PH) value is adjusted to 6-7, filtering is performed by a precise filter which is 1 mum in precision, a common reverse osmosis membrane system is used for concentration, an electroosmosis system is used for desalination, a reverse osmosis (RO) concentrated solution serves as water supply of an electroosmosis fresh water chamber, a large part of RO dialysate directly reaches the standards to be discharged, a small part of the dialysate serves as a water supply of an electroosmosis concentrated water chamber and an electrode water chamber, inorganic salt in a fresh water chamber concentrated solution is continuously moved to the concentrated water chamber under the action of an electroosmosis electric field, a fresh water chamber low-pressure RO concentrated solution after desalination is delivered into a high-pressure RO system to be subjected to concentration to obtain a high-pressure RO concentrated solution, high-pressure RO dialysate reaches the standards to be discharged, and finally, evaporation is performed in an evaporation process. By means of the oxytetracycline wastewater treatment process, water outlet COD is smaller than 100mg / L, discharging up to standards can be directly performed, environmental protection can be achieved, and costs are saved.

The invention relates to the technical field of veterinary drugs, in particular to a preparation method of doxycycline intermediate 11[alpha]-chloromethene oxytetracycline. According to the preparation method, 11[alpha]-chloromethene oxytetracycline is taken as a raw material, dichloromethane is used as a solvent, and 1,1-dichloromethyl-N,N-dimethylamine is taken as a dehydrating agent to carry out reactions to obtain 11[alpha]-chloromethene oxytetracycline. By utilizing the preparation method, the production potential safety hazard can be reduced, the product yield is high, the generated waste salt is reduced, and the equipment type selection requirement is reduced.

The invention provides a preparation method of doxycycline hydrochloride. The preparation method of the present invention comprises: 11a-chloro-6-methine oxytetracycline p-benzenemethanesulfonate hydrogenation reaction under catalyst and poison conditions; then, react with sulfosalicylic acid to obtain α-6-deoxyoxytetracycline Sulfosalicylate; after reacting with hydrogen chloride-ethanol solution, add water to stir and crystallize to obtain doxycycline hydrochloride; wherein, the poison includes: a mixture of sulfur-containing and nitrogen-containing organic compounds. The method of the invention can improve the doxycycline hydrochloride yield and stereoselectivity, and obtain high-quality raw materials.

The invention provides an aptamer electrode for oxytetracycline detection, which is characterized in that the glassy carbon electrode is sequentially decorated with graphene-gold nanocomposites and BSA-OTC complexes from bottom to top. The signal layer is a nucleic acid aptamer modified by methylene blue and folded into a G quadruple structure under the RCA chain amplification reaction. The content of the target is reflected by the amount of methylene blue, and its preparation method is provided at the same time. The preparation method of the present invention is simple and has high performance. It is stable and has good electrode repeatability, and is suitable for the detection of oxytetracycline in drug safety and the practical application of biosensor industrialization.

The invention belongs to the technical field of environmental monitoring, and relates to an oxytetracycline fluorescence detection method based on surface passivation and covalent coupling. The covalent coupling between the surface of two-dimensional MOF nanosheets and DNA can effectively utilize the limited adsorption sites on the surface of MOF nanosheets, enhance the ability of nanosheets to adsorb DNA molecules, and reduce the background fluorescence signal. At the same time, the use of short-chain DNA molecules as signal reporter molecules can effectively improve the fluorescence recovery efficiency, while the addition of surface passivator sodium polystyrene sulfonate in the reaction system further weakens the adsorption ability of nanosheets to signal reporter molecules, making MOF nanosheets The chip sensor improves the linear response to low concentration OTC and enhances the detection performance of OTC. The detection method of the invention can effectively reduce the fluorescence background value, increase the signal response value induced by the target, and effectively improve the detection performance of the fluorescent sensing platform constructed based on the principle of physical adsorption for oxytetracycline.

The present invention provides a kind of preparation method of doxycycline hydrochloride and the doxycycline hydrochloride prepared by this method, provide raw material through the reaction of chlorinated antipyretic ice and oxytetracycline, improve the productive rate of doxycycline hydrochloride, The generation of by-products is reduced, and the antipyretic ice, ethanol, methanol, etc. in the preparation process can be recycled, environmental pollution and resource waste are reduced, and the production cost is reduced, and the product yield in the preparation process of the method is stable. In addition, the doxycycline hydrochloride prepared by the preparation method of the present invention has high purity and good color.

The invention discloses a high-content oxytetracycline injection and a preparation method thereof, belonging to the field of oxytetracycline injections for veterinary use. Every 100 mL of the oxytetracycline injection comprises 30 g to 35 g of oxytetracycline, 2.0 g to 3.0 g of magnesium oxide, 0.3 g to 1.0 g of sodium formaldehyde sulfoxylate, 20 g to 40 g of glycerolformal, 20 g to 40 g of N,N-dimethylacetamide, and 1.5 g to 2.0 g of monoethanolamine, with the balance being water for injection. The preparation method comprises the following steps: (1) adding a proper amount of water for injection to dissolve sodium formaldehyde sulfoxylate; (2) adding magnesium oxide and carrying out stirring to uniformly disperse the magnesium oxide; (3) continuing adding glycerolformal, N,N-dimethylacetamide and monoethanolamine; (4) introducing nitrogen, carrying out heating to 50-60 DEG C, adding oxytetracycline alkali, and carrying out stirring to dissolve the oxytetracycline alkali; (5) addingmonoethanolamine to adjust the pH value of the obtained solution to 8.4-8.7; and (6) supplementing water for injection until a volume is 100 mL. According to the invention, an optimal ratio is selected by screening the ratios of glycerolformal to dimethylacetamide; and it is verified that the oxytetracycline injection is good in stability, not prone to discoloring and content reduction after beingplaced for a long time, and free of sediments.

The invention relates to a colorimetric aptamer sensor based on nanogold-DNA and a method for detecting oxytetracycline by using the colorimetric aptamer sensor, and belongs to the technical field ofenvironmental monitoring. The colorimetric aptamer sensor provided by the invention comprises nanogold and an OTC aptamer. The method for detecting oxytetracycline by applying the colorimetric aptamersensor comprises the following steps: 1, preparing the nanogold; 2, preparing the OTC aptamer; 3, mixing the nanogold and the OTC aptamer. The colorimetric sensor designed by the invention has the advantages of simplicity and convenience in operation, short detection time, high sensitivity, good selectivity, low detection limit, macroscopic property and the like, and is superior to other traditional OTC detection methods. The aptamer sensor disclosed by the invention can be successfully used for detecting oxytetracycline in an actual egg sample and detecting a recovery rate result of OTC in the egg sample, so the detection result is satisfactory; and a certain reference is provided for applying an aptamer-nanogold colorimetric method to detection of small molecules in foods.

The invention discloses a method for regulating and controlling compound heavy metal forms of municipal refuse compost by adopting OTC (oxytetracycline). According to the method, a PVC (polyvinyl chloride) pipe, of which the diameter is equal to 3cm, the height is equal to 25cm, is adopted, and 160 g of compost is added into the pipe; 40ml of aqueous solution with the mass concentration of 10 to 20 mmol / kg is added into the compost, wherein the temperature is 19 to 27 DEG C, and the relative humidity is 60% to 72% during the test, and water is supplemented to the compost every day under a natural light condition. A weighing method is adopted for adjusting water content, so as to keep the constant of the water content and make the water content in compost substrate reach to the maximum water-holding capacity of 70%, after curing for 45 days, sampling is carried out to analyze the forms of heavy metal. Experimental results show that after adding the OTC, the ratios of the exchangeable forms of Cd, Cr, Cu, Pb and Zn to the bound form of carbonate are all remarkably increased. By adding the OTC, the bio-available forms of the heavy metal in the compost are increased, and the non-bio-available forms are reduced, and activation is realized for the heavy metal in the compost.