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Method for extracting bacteriostatic substances from oil-tea camellia through high-speed countercurrent chromatography and application

A technology of high-speed countercurrent chromatography and antibacterial substances, which is applied in the field of separation and purification of high-speed countercurrent chromatography, and can solve the problems that the strongest antibacterial active substances have not been reported yet.

Inactive Publication Date: 2016-03-30
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the application of the HSCCC method to separate and prepare the research on the strongest antibacterial active substances in camellia oleifera has not yet been reported.

Method used

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  • Method for extracting bacteriostatic substances from oil-tea camellia through high-speed countercurrent chromatography and application
  • Method for extracting bacteriostatic substances from oil-tea camellia through high-speed countercurrent chromatography and application
  • Method for extracting bacteriostatic substances from oil-tea camellia through high-speed countercurrent chromatography and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Effect of ethanol concentration on the antibacterial activity of continuous phase change extraction of antibacterial substances in camellia oleifera

[0089] (1) Raw material processing: pulverize camellia seed meal to 20-80 mesh to obtain tea powder raw material.

[0090] (2) Continuous phase change extraction: 1.5 kg of the tea powder raw material obtained in step (1) is packed into an extraction kettle, and the concentration is 50%, 60% , 70%, 80%, and 90% ethanol extractants are compressed into liquids, and under the conditions of extraction temperature 80°C and extraction pressure 0.4MPa, flow through the extraction kettle at a flow rate of 100L / h, and continuously extract for 2h to extract the pH in Camellia oleifera. After decomposing the bacterial substances, they flowed through the desorption kettle under the conditions of the desorption temperature of 80°C and the desorption pressure of 0.2MPa to obtain 287g, 293g, 300g, 310g and 303g of antibacteria...

Embodiment 2

[0106] Example 2 The influence of extraction pressure on the antibacterial activity of antibacterial substances in Camellia oleifera extracted by continuous phase change extraction

[0107] For further illustrating the beneficial effect of the present invention, repeat the step of embodiment 1, it is 80% that the ethanol extraction agent of 50%, 60%, 70%, 80%, 90% in embodiment 1 step (2) is replaced into concentration Ethanol extractant, the extraction pressure 0.4MPa in embodiment 1 step (2) is replaced with 0.3MPa, 0.4MPa, 0.5MPa, 0.6MPa, 0.7MPa, respectively obtains the bacteriostatic substance 291g, 302g, 311g, 301g in camellia oleifera , 297g, specifically as shown in Table 2.

[0108] The experimental results show that with the gradual increase of the extraction pressure of the system, the diameter of the inhibition zone changes, but the regularity is not obvious. When the pressure reaches 0.5MPa, the extract has no effect on E. The antibacterial effect of Aspergillus ...

Embodiment 3

[0111] Example 3 The influence of extraction temperature on the antibacterial activity of antibacterial substances in Camellia oleifera extracted by continuous phase change extraction

[0112] For further illustrating the beneficial effect of the present invention, repeat the step of embodiment 1, it is 80% that the ethanol extraction agent of 50%, 60%, 70%, 80%, 90% in embodiment 1 step (2) is replaced into concentration Ethanol extractant, replace the extraction temperature 80°C in step (2) of Example 1 with 50°C, 60°C, 70°C, 80°C, and 90°C to obtain 293g, 296g, 302g, and 317g of antibacterial substances in Camellia oleifera , 310g, specifically as shown in Table 3.

[0113] The experimental results show that: with the gradual increase of the extraction temperature, the inhibitory zone of the extract against Escherichia coli, Staphylococcus aureus and Aspergillus niger increases, and when the temperature exceeds 80°C, it shows a gradual downward trend, which may be due to a ...

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Abstract

The invention provides a method for extracting bacteriostatic substances from oil-tea camellia through high-speed countercurrent chromatography and an application and also provides a bacteriostatic extract of oil-tea camellia and two bacteriostatic compounds of oil-tea camellia. Through optimization of the operating condition of the high-speed countercurrent chromatography, three bacteriostatic active substances of the oil-tea camellia are separated, the operation is simple, the purity of obtained monomers is high, quick and efficient separation of the bacteriostatic active substances is realized, and the separated bacteriostatic active substances can effectively bacteria and fungi.

Description

technical field [0001] The invention relates to the field of separation and purification of high-speed countercurrent chromatography, in particular to a method and application of high-speed countercurrent chromatography for extracting antibacterial substances in camellia oleifera, and also relates to an extract of camellia oleifera. Background technique [0002] At present, most of the active ingredients in plant sources are extracted by water extraction, steam distillation, organic solvent method, ultrafiltration membrane method, etc. These methods have low yield, long extraction time, many processes, and energy Disadvantages such as large consumption and low purity. [0003] High-speed countercurrent chromatography is a new type of liquid-liquid partition chromatography technology, which can achieve high-efficiency distribution of samples in two immiscible solvent systems in a short period of time, thereby achieving a high degree of separation of samples. The biggest adva...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P1/00A01P3/00C07H17/07C07H1/08
CPCA01N65/00A01N65/08C07H1/08C07H17/07
Inventor 丘苑新曹庸翟万京赵丽柳建良
Owner ZHONGKAI UNIV OF AGRI & ENG
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