Corn oil authentication method adopting stable carbon isotope technique

A carbon isotope and corn oil technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of misjudgment, difficulty in identification of adulteration as oil, interference, etc. Effect

Inactive Publication Date: 2016-03-30
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

Domestic research in this field started relatively late. Up to now, only Jin Qingzhe and others have used the difference of carbon isotopes of fatty acids in oils to detect palm oil in corn oil; Guo Lianxian and others have used the carbon isotope composition of triglycerides and carbon The isotope composition is used to detect the adulteration of vegetable oils, but this technology has certain disadvantages: triglycerides are composed of glycerol and fatty acids, but the types and contents of fatty acids in different varieties of oils are quite different, and even It is the same species, but the content of fatty acid is also different in different places of origin. According to the principle of isotope metabolic fractionation, different compounds have different carbon isotope composition due to the different chemical reactions involved in the process of biological metabolism. Therefore, the triglyceride The carbon isotope composition is difficult to be used as a technical index for the identification of adulteration in oils; the carbon isotope composition of fatty acids is an important technical index for the current research and application of adulteration detection. Sometimes it is not just simply adding cheap oil to high-priced oil, but also adding essence, aeruginosa and other substances, so that the type and content of fatty acid are as consistent as possible with pure oil. In this case, the carbon isotope composition of fatty acid is in The application process will be greatly disturbed, resulting in wrong judgments

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  • Corn oil authentication method adopting stable carbon isotope technique
  • Corn oil authentication method adopting stable carbon isotope technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] δ in glycerol 13 Determination of C value

[0023] 1.1 Sample pretreatment

[0024] Weigh 0.12 g of vegetable oil and dissolve it in 8 mL of isooctane; add 400 μL of supersaturated methanolic potassium hydroxide solution, oscillate fully, let stand and remove the upper layer solution, add about 70 μL concentrated hydrochloric acid to the lower layer solution to neutralize potassium hydroxide and keep Acidity, the solution is layered to remove the upper layer solution (mainly free fatty acid) again, add 50 μL of supersaturated potassium hydroxide methanol solution to the lower layer solution to neutralize concentrated hydrochloric acid, add 1 mL of methanol after the salt is precipitated, and dilute the methanol upper layer solution containing glycerin Transfer to a 2mL reagent bottle for use.

[0025] 1.2 Chromatographic conditions

[0026]CP-Wax capillary column (25m*0.25mm*0.20μm); the carrier gas is helium; the column flow rate is 1.2mL / min; the inlet temperature ...

Embodiment 2

[0036] Example of corn oil sample identification

[0037] 2.1 Collect 20 samples of corn oil from different origins and whose authenticity is known, and 10 samples of corn raw materials from different origins. The corn raw material samples are physically pressed to extract oil under laboratory conditions; 2.2 Collect sunflower oil with known authenticity , one sample each of rapeseed oil, soybean oil, peanut oil, olive oil and animal fat;

[0038] 2.3 Purchase 3 samples marked as corn oil from the market;

[0039] 2.4 δ in glycerol of oil 13 C value determination:

[0040] 2.4.1 Sample pretreatment

[0041] Weigh 0.12g of vegetable oil and dissolve it in 8mL of isooctane; add 400μL of supersaturated methanolic potassium hydroxide solution, oscillate fully, let stand and remove the upper layer solution, add about 70μL concentrated hydrochloric acid to the lower layer solution to neutralize potassium hydroxide and keep Acidity, the solution is layered to remove the upper lay...

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Abstract

The invention discloses an authentication method for edible corn oil and particularly provides a corn oil authentication method adopting a stable carbon isotope technique. The corn oil authentication method is established according to a natural fractionation characteristic of the stable carbon isotope, a plant anabolism theory, a transesterification technology and the stable carbon isotope analysis technique. The distribution range of delta<13>C values of glycerin of the corn oil is established through determination of the delta<13>C values of glycerin of triglyceride in grease, and the authenticity of a to-be-detected sample is analyzed according to the principle of statistics. The corn oil authentication method is established by adopting the technique for analyzing the stable carbon isotope of the glycerin in the grease, the technical problem for detection of illegal addition of low-priced grease to the corn oil existing in the edible oil market in China is solved, the consumer benefit is protected, and healthy and ordered development of the edible oil market is promoted.

Description

Technical field: [0001] The invention belongs to the field of edible oil quality detection. In particular, it relates to a method for identifying the authenticity of corn oil by using a stable carbon isotope technique. Background technique: [0002] Edible vegetable oil is an indispensable necessity in daily life, and the prices of different types of vegetable oil vary greatly due to their different nutritional values. Corn germ oil is commonly eaten by people in the north. However, due to the full liberalization of the grain purchase and sales market in recent years, palm-derived oil and other oils and fats with lower prices have become the target of some unscrupulous traders in pursuit of adulteration. In corn oil, incidents of counterfeit pure corn oil sales occur from time to time. [0003] The identification of the authenticity of edible vegetable oil is a technical problem faced by all countries in the world. It has attracted the attention of scientific and technolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 钟其顶陈姗姗王道兵
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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