Method for preparing cage-shaped gold nanoparticles through lanreotide acetate template

A technology of lanreotide acetate and gold nanoparticles, applied in the field of materials, can solve the problems of uneven particle surface voids, low mechanical strength resistance, and uncontrollable morphology, and achieves good reproducibility, good binding condition, Effects that are easy to analyze and control

Active Publication Date: 2016-04-06
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in previous preparation studies, due to the special morphology and special bond interactions of gold nanocages, its morphology is not easy to control, the gaps on the surface of the particles are not uniform, the resistance to mechanical strength is low, and the purity of the product is low.
Moreover, these preparation methods generally have the disadvantages of polluting the environment, complicated process, and high cost.

Method used

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  • Method for preparing cage-shaped gold nanoparticles through lanreotide acetate template
  • Method for preparing cage-shaped gold nanoparticles through lanreotide acetate template
  • Method for preparing cage-shaped gold nanoparticles through lanreotide acetate template

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 0.5 mg of lanreotide acetate (manufactured by Wuxi Maimertop Biotechnology Co., Ltd.), and dissolve it in 1 mL of hydrochloric acid solution at pH 2.0 to prepare a lanreotide acetate solution. 20min in the bath, and then ultrasonic treatment for 15s, the ultrasonic frequency is 40Hz; 200μL of lanreotide acetate solution after the metal bath was mixed with 200μL of 2.0mM gold trichloride solution (Tianjin Chemical Reagent Factory) and placed in a water bath constant temperature oscillator Incubate for 16 hours at a control speed of 100 rpm and a temperature of 25°C; finally add 100 μL of 1 mM sodium borohydride (produced by Beijing Zhongsheng Huateng Technology Co., Ltd.) dropwise for reduction, the dropping speed is 2 drops / min, 25 μL / drop , the reaction temperature was 22°C, and the reaction was carried out for 5 minutes, so that it slowly changed from light yellow to blue-purple, that is, lanreotide acetate-caged gold nanoparticles were obtained.

[0021] The mor...

Embodiment 2

[0023] Take 0.75 mg of lanreotide acetate and dissolve it in 1 mL of hydrochloric acid solution at pH 4.0 to prepare a lanreotide acetate solution. Place the lanreotide acetate solution in a metal bath at 70°C for 30 minutes, and then perform ultrasonic treatment for 15 seconds. 40Hz; take 200μL of lanreotide acetate solution after the metal bath and mix with 200μL of 5.0mM gold trichloride solution, put it into a constant temperature oscillator in a water bath, control the speed at 150rpm, and incubate at 30℃ for 20h; finally drop by drop Add 100 μL of 2.5 mM sodium borohydride for reduction, the dropping rate is 2 drops / min, 25 μL / drop, the reaction temperature is 23°C, and react for 10 minutes, so that it slowly changes from light yellow to blue purple, and the lanreotide acetate-cage is obtained shaped gold nanoparticles.

[0024] The morphology of caged gold nanoparticles was characterized by transmission electron microscopy, such as figure 2 As shown, the diameter of t...

Embodiment 3

[0026] Take 1.0 mg of lanreotide acetate and dissolve it in 1 mL of hydrochloric acid solution at pH 6.0 to prepare a lanreotide acetate solution. Place the lanreotide acetate solution in a metal bath at 70°C for 40 minutes, and then perform ultrasonic treatment for 15 seconds. 40Hz; take 200μL of lanreotide acetate solution after the metal bath and mix with 200μL of 7.0mM gold trichloride solution, put it into a constant temperature oscillator in a water bath, control the rotation speed at 130rpm, and incubate at 30℃ for 24h; finally drop by drop Add 100 μL of 5.0 mM sodium borohydride for reduction, the dropping rate is 2 drops / min, 25 μL / drop, the reaction temperature is 25°C, and react for 15 minutes, so that it slowly changes from light yellow to blue purple, and the lanreotide acetate-cage is obtained shaped gold nanoparticles.

[0027] The morphology of caged gold nanoparticles was characterized by transmission electron microscopy, such as image 3 As shown, the diamet...

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Abstract

The invention discloses a method for preparing cage-shaped gold nanoparticles through a lanreotide acetate template. Mainly, according to the proportion that 0.5-1.0 mg of lanreotide acetate is added in each milliliter of a hydrochloric acid solution with the pH of 2-6, a lanreotide acetate solution is prepared and placed in a metal bath at the temperature of 70 DEG C for 20-40 min, and ultrasonic treatment is carried out; an auric chloride solution is added in the lanreotide acetate solution, the molar ratio of the auric chloride solution and the lanreotide acetate solution is 1:4-8, the mixture is placed in a water bath constant-temperature oscillator, the rotating speed is 100-150 rpm, and incubation at the temperature of 25-30 DEG C is conducted for 16-24 h; reducing agent sodium borohydride is added in the incubated solution, the molar ratio of the reducing agent sodium borohydride to the auric chloride solution is 1:2-4, the dripping speed is controlled at 2 drops / min, the reaction temperature is about 22-25 DEG C, the reaction time is 5-15 min, and the cage-shaped gold nanoparticles with the grain size of 80-120 nm and good dispersibility are obtained. The method has the advantages that raw materials are low in price and easy to obtain, the device is simple, operation is easy, the reaction conditions are moderate, the appearance is controllable, and repeatability is high.

Description

technical field [0001] The invention belongs to the technical field of materials, and in particular relates to a preparation method of nanometer noble metal materials. Background technique [0002] Gold nanomaterials not only have the general properties of nanomaterials, but also have unique optical and electrical properties and good biocompatibility. A gold nanocage is assembled from a large number of individual gold nanoparticles, and its shape is a hollow, porous three-dimensional particle on the wall. The special cage shape increases its surface area, and at the same time endows itself with more Therefore, it has more superior performance in many fields such as drug loading, fluorescent labeling, and biological detection. In addition, gold nanocages have excellent optical properties, and their surface plasmon resonance peaks can undergo a large red shift, which can be used for photothermal therapy of tumors. Through chemical surface modification and other modification ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB82Y40/00B22F9/24B22F1/0549B22F1/054
Inventor 高大威薛伟利李彦集高发明王梓边可新尹甜
Owner YANSHAN UNIV
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