Optimized method for ultrasonic assisted enzymatic extraction of duck ossein
An auxiliary extraction and ultrasonic technology, applied in the direction of food science, etc., can solve the problem of patent application for no extraction method of duck bone
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[0027] Fresh duck bones from Guizhou Sansui are used, the meat is removed first, washed with clean water, drained and set aside; then the dried duck bones are crushed with a HC-300T2 high-speed grinder for 5 minutes; then the duck bone powder is sent to the KQ-300DE CNC Add water to the ultrasonic cleaner and add 800u / mg of papain, trypsin, alkaline protease, and neutral protease at the same time to make it enzymatically hydrolyze. Ostein was extracted from the enzymolysis solution, the ultrasonic extraction temperature in the ultrasonic cleaning machine was 70°C, the ultrasonic power was 210W, and the extraction time was 3h. The extract was sent to a GL-20G high-speed refrigerated centrifuge to separate the liquid phase, and the process conditions were controlled at a temperature of 4°C; the speed of the centrifuge was 5000r / min, and the centrifugation time was 15min. clarify. Concentrate the supernatant obtained after clarification of the liquid phase with a RE-52A rotary e...
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