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A process for extracting heparan sulfate from duodenum

A technology of heparan sulfate and duodenum, applied in the field of biomedicine, can solve the problems of uncontrollable product quality of heparan sulfate, inability to effectively improve yield, low chemical purity, etc., and achieve controllable product quality and suitable for large-scale production. Production, the effect of high chemical purity

Active Publication Date: 2018-03-16
DONGYING TIANDONG PHARM CO LTD
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Problems solved by technology

[0003] The purpose of the present invention is to provide a process for extracting heparan sulfate from the duodenum, aiming to solve the problems of uncontrollable quality of current heparan sulfate products, complex preparation process, low chemical purity, and inability to effectively increase the yield

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  • A process for extracting heparan sulfate from duodenum

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[0061] Option One:

[0062] ⑴ Thaw 1Kg pig duodenum, remove excess fat, wash and mince to obtain intestinal pulp.

[0063] ⑵. Add 3000mL of water under stirring, adjust the temperature to 60°C, pH to 10.0, add 30g of Novozymes protease, and perform enzymatic hydrolysis for 8 hours.

[0064] (3) Adjust the pH to 7.0, raise the temperature to 90°C, and heat for 10 minutes.

[0065] ⑷. Cool the feed solution to room temperature, centrifuge, and collect the centrifugate.

[0066] ⑸. Add 300mL of A98 resin, and absorb in a 60℃ water bath for 5 hours.

[0067] ⑹. Clean the adsorbed resin, add 900mL of 2.5% sodium chloride solution, add in three times, stir for 30 minutes each time, and collect the eluent. Then add 900 mL of 6% sodium chloride solution, add in three times, stir for 60 minutes each time, and collect the eluate.

[0068] ⑺. Add 45g of sodium chloride to the collected 6% eluent, and add 1300mL of medicinal ethanol for precipitation.

[0069] (8) Dissolve the precip...

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Abstract

The invention discloses a technology for extracting heparin sulfate from duodena. The duodena of pigs serve as raw materials in the method, bio-enzyme and acid and alkali treatment methods are combined in the technology, and heparin sulfate meeting the specification is obtained through obtaining of the duodena of pigs, mincing, enzymolysis, heating, centrifuging, resin adsorbing, eluting, settling, alkali heating, acid treatment, low-power settling, oxidation, settling and vacuum drying. The method and technology are easy to implement, low in production cost, high in product stability and suitable for mass production. The product is controllable in quality and high in chemical purity, the preparing technology is advanced, and the yield can be effectively increased.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a process for extracting heparan sulfate from duodenum. Background technique [0002] Heparan sulfate is an important class of substances in animal tissues. Heparan sulfate is similar to heparin in structure to a certain extent, and both belong to mucopolysaccharides. Although heparin has been widely used clinically, there are still relatively large side effects such as bleeding in clinical practice, which limits its application to a certain extent. However, heparan sulfate overcomes the side effects of heparin application to a large extent, and at the same time has biological effects such as anti-inflammatory, antithrombotic and blood lipid lowering similar to heparin. Therefore, heparan sulfate is a promising new biochemical drug among mucopolysaccharides. For example, foreign products and a mixture of multiple mucopolysaccharides can be called heparanoids in a broad sens...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/10A61K31/727A61P29/00A61P7/02A61P3/06
CPCA61K31/727C08B37/0003C08B37/0075
Inventor 杨晓宏郭林李荣李福川陈少鹏赵娟
Owner DONGYING TIANDONG PHARM CO LTD