Soybean phosphate-starvation negative regulatory gene GmSPX1, and coding protein and application thereof

Abstract

The invention belongs to the technical field of gene engineering, and particularly discloses a phosphate-starvation negative regulatory gene GmSPX1, and a coding protein and application thereof. The nucleotide sequence of the gene is disclosed as Seq ID NO.1. The invention detects that any plant expression vector capable of inserting the exogenous gene GmSPX1 can be used for transforming plant cells to obtain the GmSPX1-overexpressed transgenic plant. Compared with the non-transgenic plant, the complete phosphorus content of the transgenic plant is obviously reduced, the phosphate-starvation related gene is obviously lowered, the root system morphology is obviously changed, and the phosphorus poisoning phenomenon of the mutant is relieved. The gene disclosed by the invention can be inserted into the plant as a target gene, enhances the phosphorus in-vivo metabolic balance capacity of the transgenic plant, and thus, has important meanings for culturing phosphorus-efficient-utilization soybean varieties.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a soybean phosphorus starvation negative regulation gene GmSPX1, encoded protein and application thereof. Background technique [0002] Among the 17 elements necessary for plant growth and development, phosphorus (P) is a very important one. P element is inseparable from plant cell activities including ribonucleic acid synthesis, cell membrane construction and ATP synthesis. Even many complexes involved in enzymatic reactions and signal transduction processes must contain P elements. Plant roots can only obtain the necessary P element by absorbing inorganic phosphate (Pi) in the soil. However, due to the negative charge of the soil, Pi is easily leached, and most of the Pi in the soil is either converted into organic compounds by microorganisms, or becomes insoluble in water by interacting with cations, resulting in a large amount of P being fixed. Cann...

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Problems solved by technology

[0006] Similar results were obtained in the study of rice OsSPX; although the expression level of OsSPX4 did not change significantly under the condition of phosphorus starvation stress, it was found that the expression level of the PSI gene in the OsSPX4 mutant was significantly increased, and the plant total phosphorus The content will also increase significantly; experiments such as yeast two-hybrid experiment and BiFC found that OsSPX4 can interact with OsPHR2, and under normal phosphorus environment, OsSPX4 can inhibit the activity of OsPHR2, but under low phosphorus environment, OsSPX4 will be inhibited by the proteasome pathway. Degradation and inactivation; recent reports also found that OsSPX1 and OsSPX2 have similar effects. Under normal phosphorus environment, OsSPX1 / OsSPX2 and OsPHR2 have high affinity, but under phosphorus starvation stress, OsSPX1 / OsSPX2 and OsPHR2 will become Low affinity; In the study of OsSPX3 and OsSPX5, it was found that they can not only negatively regulate the process of rice Pi translocation from the underground to the shoot, but also inhibit the expression of OsPHR2, but there is no direct evidence of interaction

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