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Preparation method for 6-mercaptopurine molecularly-imprinted electrochemical sensor

A technology of molecular imprinting and mercaptopurine, which is applied in the direction of scientific instruments, instruments, and analytical materials, can solve problems such as poor regeneration and reversibility, high detection limit, and difficulty in controlling the thickness of the imprinted film, so as to improve the response signal, improve the conductivity, high affinity effect

Inactive Publication Date: 2016-04-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Glassy carbon electrode pretreatment: the glassy carbon electrode 0.05μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with twice distilled water, washed several times with absolute ethanol, dried with nitrogen, and soaked in γ--(2,3-epoxy Propoxy) Propyltrimethoxysilane in absolute ethanol solution, the temperature rises to 50±2°C and soak for 70min, after taking it out, wash it several times with absolute ethanol, dry it, and get the pretreated glassy carbon electrode;

[0023] (2) Preparation of nano-platinum-modified glassy carbon electrode: put the pretreated glassy carbon electrode into 0.12mol / L platinum chloride aqueous solution, soak for 3 hours at room temperature, take it out, wash it with deionized water, and then put it in the mass percent concentration Soak in 25% hydrazine hydrate solution for 1 hour, take it out, wash it with deionized water, and dry it naturally to get nano-platinum modified glassy carbon electrode; ...

Embodiment 2

[0027] (1) Glassy carbon electrode pretreatment: the glassy carbon electrode 0.05μm Al 2 o 3The surface of the powder was polished, then ultrasonically cleaned with twice distilled water, washed several times with absolute ethanol, dried with nitrogen, and soaked in γ--(2,3-epoxy Propoxy) Propyltrimethoxysilane in absolute ethanol solution, the temperature rises to 50±2°C and soak for 80min, after taking it out, wash it several times with absolute ethanol, dry it, and get the pretreated glassy carbon electrode;

[0028] (2) Preparation of nano-platinum-modified glassy carbon electrode: Put the pretreated glassy carbon electrode into 0.12mol / L platinum chloride aqueous solution, soak it at room temperature for 2h, wash it with deionized water after taking it out, and then put it into the mass percent concentration Soak in 25% hydrazine hydrate solution for 1 hour, take it out, wash it with deionized water, and dry it naturally to get nano-platinum modified glassy carbon electr...

Embodiment 3

[0032] (1) Glassy carbon electrode pretreatment: the glassy carbon electrode 0.05μm Al 2 o 3 The surface of the powder was polished, then ultrasonically cleaned with double distilled water, washed several times with absolute ethanol, dried with nitrogen, and soaked in γ--(2,3-epoxy Propoxy) Propyltrimethoxysilane in absolute ethanol solution, the temperature rises to 50±2°C and soak for 60min, after taking it out, wash it several times with absolute ethanol, dry it, and get the pretreated glassy carbon electrode;

[0033] (2) Preparation of nano-platinum-modified glassy carbon electrode: Put the pretreated glassy carbon electrode into 0.12mol / L platinum chloride aqueous solution, soak it at room temperature for 4h, wash it with deionized water after taking it out, and then put it in the mass percent concentration Soak in 25% hydrazine hydrate solution for 1 hour, take it out, wash it with deionized water, and dry it naturally to get nano-platinum modified glassy carbon electr...

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Abstract

The invention discloses a preparation method for a 6-mercaptopurine molecularly-imprinted electrochemical sensor. The preparation method is characterized by comprising the following steps: modifying a glassy carbon electrode with a silane coupling agent and nanometer platinum; then adding, by mass percentage concentration, 10 to 20% of dimethacrylic acid-1,4-butanediol ester, 8 to 18% of methyl acrylate, 8 to 18% of maleic anhydride, 50 to 60% of N,N-dimethyl formamide 1.0 to 3.0% of dimethyl azodiisobutyrate and 1.0 to 3.0% of 6-mercaptopurine into a reactor, introducing argon for 5 min to remove oxygen and preparing a 6-mercaptopurine molecularly-imprinted polymer in an argon atmosphere; and coating the modified electrode with the polymer and removing template molecule so as to obtain the 6-mercaptopurine molecularly-imprinted electrochemical sensor. The sensor has the advantages of high 6-mercaptopurine recognition performance, low cost, high sensitivity, good specificity, rapid detection, reusability and improved response to 6-mercaptopurine.

Description

technical field [0001] The present invention relates to a preparation method of a molecular imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of a 6-mercaptopurine molecularly imprinted electrochemical sensor, specifically based on the specific recognition of molecular imprinting, for detection 6-mercaptopurine technology in pharmaceuticals, food, biological samples. Background technique [0002] 6-mercaptopurine (6-MP) is an antineoplastic drug, which is effective for acute leukemia and also effective for chronic myeloid leukemia; it is used for choriocarcinoma and malignant mole. In addition, it also has a certain effect on malignant lymphoma and multiple myeloma. The mechanism of action of 6-mercaptopurine: The chemical structure of 6-mercaptopurine is similar to that of hypoxanthine, the only difference is that the hydroxyl group is replaced by a sulfhydryl group on the 6-position C in the molecule. 6-mercapt...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 李慧芝李冬梅卢燕
Owner UNIV OF JINAN
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