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A method for enriching 1-deoxynojirimycin in mulberry leaves and the obtained ultrafine powder

A technology of deoxynojirimycin and ultra-fine powder, which is applied in the field of food biology, can solve the problems of side effects and single diabetes, and achieve the effects of low side effects, high preservation rate and low cost

Active Publication Date: 2018-08-14
镇江市智农食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to propose a technical method for enriching 1-deoxynojirimycin (DNJ) in mulberry leaves and its ultrafine powder in view of the single food suitable for diabetics on the market and the side effects of drug treatment.

Method used

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  • A method for enriching 1-deoxynojirimycin in mulberry leaves and the obtained ultrafine powder
  • A method for enriching 1-deoxynojirimycin in mulberry leaves and the obtained ultrafine powder
  • A method for enriching 1-deoxynojirimycin in mulberry leaves and the obtained ultrafine powder

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Select fresh and complete tender mulberry leaves, remove the stalks and clean them, cut them into 8 pieces, soak them in 20g / L glucose solution at 40°C, take them out after 3 hours, drain the sugar solution, and then dry them in a hot air drying oven at 60°C until The water content is less than 5%, and the plant tissue grinder is used for coarse grinding to more than 80 mesh, and the vibration ball mill is used for 11 hours to grind to an average particle size of 5 μm, and dried in an oven at 50°C to reduce the water content to less than 5%. After the dried product is cooled to room temperature, it is packed with nitrogen.

Embodiment 2

[0033] Select fresh and complete mulberry leaves, remove the stalks and clean them, cut them into 6 pieces, soak them in 15g / L glucose solution, and dry them in a hot air drying oven at 65°C until the water content is below 5% after 4 hours at 35°C , use a plant tissue pulverizer to coarsely pulverize to more than 80 mesh, use a vibrating ball mill to pulverize for 9 hours to an average particle size of 8 μm, and then dry in an oven at 50°C to reduce the moisture content to below 5%. After the dried product is cooled to room temperature, it is packed with nitrogen.

Embodiment 3

[0035] Wash and dry the fresh and complete tender mulberry leaves and cut them into several flake fragments, immerse the mulberry leaf fragments in the glucose solution for a certain period of time, take out the mulberry leaf fragments and dry them until the water content of the mulberry leaves is below 5%, and then coarsely crush them to More than 80 meshes, pulverized by a vibrating ball mill or ultrafine pulverizer, dried to reduce the water content to below 5% to obtain mulberry leaf ultrafine powder enriched in 1-deoxynojirimycin.

[0036] 1. The influence of mechanical cutting ( figure 1 )

[0037] Select mulberry leaves with similar leaf size, divide them into 5 parts, each 100g, cut them into 2 pieces, 4 pieces, 8 pieces, and 16 pieces respectively. Take the whole leaves as the control, soak them in 20g·L -1 In the glucose solution, let it stand for 2h, while the temperature is controlled at 30°C. It was found that the content of DNJ reached the highest when the cut ...

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Abstract

The present invention relates to a 1-deoxynojirimycin enriched mulberry leaf preparation method and an obtained ultra-fine powder, which belongs to the processing field of medicinal and edible food. Fresh and complete tender mulberry leaves are washed, air-dried and cut into slice shapes with a length and width of 3-6 cm*3-6 cm, the mulberry leaf broken slices are soaked in a glucose solution at a temperature of 30-60 DEG C and a concentration of 10-30 g / L for 3-5 hours, the taken mulberry broken slices are dried to a water content to below 5%, the dried mulberry broken slices are crushed into an average particle size of 8 [mu]m or less, and the crushed mulberry leaves are dried to a water content to below 5%, thereby obtaining the 1-deoxynojirimycin enriched mulberry leaf ultra-fine powder. During the fresh mulberry leaf soaking process, endogenous DNJ synthesis related enzymes are activated and the glucose is catalyzed to enrich DNJ. The highest content of the finished product of the DNJ is 250mg / 100g. The obtained product by the method is clear in health-care function and safe and reliable in consumption.

Description

technical field [0001] The present invention relates to a method for manufacturing mulberry leaf powder rich in DNJ and its products, and the specific content relates to a method for preparing mulberry leaf powder rich in DNJ by using enzyme biotechnology in the process of soaking fresh mulberry leaves at a certain temperature , belonging to the field of food biotechnology. technical background [0002] Mulberry leaf is a food resource with rich raw material resources and health functions. It is not only rich in amino acids, unsaturated fatty acids, dietary fiber, vitamins and mineral elements such as iron, calcium, and zinc, but also rich in functional ingredients DNJ( 1-deoxynojirimycin). As a representative substance of polyhydroxyalkaloids in mulberry leaves, DNJ is the main active ingredient for lowering blood sugar. It can be used in the production of health food or medicine. It is natural, safe and reliable. At present, DNJ and its derivatives have been used as medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/105
CPCA23V2002/00A23V2200/328
Inventor 韩永斌潘艳琴王艺霖徐志宏
Owner 镇江市智农食品有限公司
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