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A nanoparticle preparation for treating HPV infection and preparation method thereof

A technology of nanoparticles and preparations, applied in the field of medicine, to achieve the effect of reducing incidence, good social value, and good HPV targeting

Active Publication Date: 2018-01-19
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Nanoparticle formulations composed of poly(beta aminoester) polymers and recombinant plasmids for HPV infection do not currently exist

Method used

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  • A nanoparticle preparation for treating HPV infection and preparation method thereof
  • A nanoparticle preparation for treating HPV infection and preparation method thereof
  • A nanoparticle preparation for treating HPV infection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 Synthetic Poly(β Urethane) Polymer

[0071] Step 1: Weigh 2.18 g (11 mmol) of 1,4-butanediol diacrylate into a beaker with an electronic balance, add 2 mL of dimethyl sulfoxide (DMSO), dissolve it with ultrasound, and stir until uniformly dispersed.

[0072] Step 2: Weigh 0.89 g (10 mmol) of 4-amino-1-butanol into a beaker, add an appropriate amount of dimethyl sulfoxide (DMSO), ultrasonically dissolve, and stir until uniformly dispersed. The obtained solution was mixed with the solution in step 1, and DMSO was added to a total volume of 18.38mL, so that the molar ratio of the weighed 1,4-butanediol diacrylate to 4-amino-1-butanol was 1.1:1, The density is 167mg / mL. It was unexpectedly found in the research that this molar ratio can produce better results.

[0073] Step 3: Add the solutions in step 1 and step 2 together into a light-proof glass flask, put the flask into a constant temperature magnetic stirring oil bath, put the liquid in the flask into ma...

Embodiment 2

[0075] Example 2 Preparation of recombinant plasmid DNA targeting knockdown or knockout HPV

[0076] The plasmid in the system of the present invention can use the short hairpin RNA (shRNA) recombinant plasmid DNA of target knockdown HPV, also can use the zinc finger ribonuclease (ZFN) recombinant plasmid DNA of target knockdown HPV, transcription activator Like Effector Nuclease (TALEN) recombinant plasmid DNA, clustered regularly interspaced short palindromic repeats (CRISPR) recombinant plasmid DNA.

[0077] HPV16 is the most common type, accounting for about 50%. Recombinant plasmid DNA targeting HPV16 includes TALEN recombinant plasmid DNA targeting knockout type 16 HPV, shRNA recombinant plasmid DNA, etc.

[0078] The present invention is constructing the recombinant plasmid DNA of targeted knockout type 16 HPV, respectively constructing 6 regularly clustered interspaced short palindromic repeat recombinant plasmid DNAs (CRISPR) and 2 short hairpin RNAs (shRNA) targetin...

Embodiment 3

[0095] Example 3 Poly(β-amino ester) polymer and targeted recombinant plasmid DNA are mixed to make nanoparticle suspension

[0096] The poly(β amino ester) polymer and the targeting recombinant plasmid DNA are suitably diluted with 25mM sodium acetate solution respectively, and after mixing, the mass ratio of the poly(β amino ester) polymer and the targeting recombinant plasmid DNA being made is 20:1 to 80:1 solution, according to the experiments of the inventors, unexpectedly found that the mass ratio in this range can produce better results, especially when the mass ratio is 60:1, the effect is better. For example, when the mass ratio is 60:1, dilute 6000 μg of poly(β-amino ester) polymer to 60 μL with 25 mM sodium acetate solution, dilute 100 μg of plasmid DNA to 60 μL with 25 mM sodium acetate solution, and mix the two solutions quickly Shake and incubate at room temperature for 15-20 minutes to prepare 120 μL of nanoparticle suspension for treating HPV infection.

[009...

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Abstract

The invention relates to a nanoparticle preparation for treating HPV (Human Papilloma Virus) infection and a preparation method of the nanoparticle preparation. The nanoparticle preparation for treating the HPV infection is prepared from a poly(beta amino ester) polymer and a recombinant plasmid DNA (Deoxyribonucleic Acid) for knocking out or knocking down the HPV in a targeting manner. The nanoparticle preparation disclosed by the invention takes the poly (beta amino ester) polymer and the recombinant plasmid DNA for knocking out or knocking down the HPV in a targeting manner as main raw materials and has the advantages of good specificity, high transfection efficiency, low toxic or side effects and the like.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a nanoparticle preparation for treating human papillomavirus (HPV) infection, in particular to a human papillomavirus treatment comprising poly(β-amino ester) polymers and recombinant plasmids targeted to knock out or knock down HPV. Nanoparticle preparations for papillomavirus (HPV) infection and methods for their preparation. Background technique [0002] Cervical cancer is a serious threat to women's health, accounting for the third place in the world's female malignant tumor diseases. More than 85% of new cervical cancer cases and cancer-related deaths occur in developing countries (Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin, 2011, 61(2): 69-90. ). [0003] High-risk human papillomavirus (HPV) infection is the main cause of cervical cancer, 16 and 18 of which two subtypes, resulting in 70% of cervical cancer. The important reason for HPV to cause ca...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/7088A61K9/14A61K47/34A61P31/20A61P35/00C08G73/00
CPCA61K9/0034A61K9/02A61K9/06A61K9/10A61K9/146A61K31/7088A61K47/34C08G73/00
Inventor 马丁汪辉祝达沈慧胡争程静
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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