Composite bioactive factor liposome and preparation method thereof

A technology of bioactive factors and liposomes, which is applied in liposome delivery, animal cells, pharmaceutical formulations, etc., can solve the problems of limited liposome application, incomplete nutritional components, and low activity of complex bioactive factors, achieving The effect of preventing ultraviolet rays, improving storage conditions, and stabilizing properties

Active Publication Date: 2016-05-04
陕西瑞妍化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] However, the liposomes disclosed in the prior art only contain a single bioactive factor, and the nutritional components are not comprehensive, which limits the application of liposomes. Moreover, the properties of the factors contained in the composite bioactive factor are different, and the applicable pH value is also different. , so, using the existing technology to adjust the method of pH value incubation, will cause the activity of complex bioactive factors to be lower

Method used

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  • Composite bioactive factor liposome and preparation method thereof
  • Composite bioactive factor liposome and preparation method thereof
  • Composite bioactive factor liposome and preparation method thereof

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preparation example Construction

[0049] On the one hand, the embodiment of the present invention provides a kind of preparation method of composite bioactive factor liposome, see figure 1 , including the steps:

[0050] Step 1. Dissolve 1-5 parts of phospholipids and 0.02-0.2 parts of cholesterol with an organic solvent, rotate and evaporate under reduced pressure, and evaporate the organic solvent to form a uniform and transparent lipid film;

[0051] Step 2, the solution containing 0.005-0.01 part of the complex biologically active factor and the uniform lipid film are rotated and hydrated to form a thick liposome of the complex biologically active factor;

[0052] Step 3, preparing the obtained composite biologically active factor crude liposomes into composite biologically active factor liposomes with a particle size of 50-100 nm.

Embodiment 1

[0066] Dissolve 0.5 part of HSPC, 0.5 part of soybean lecithin and 0.02 part of cholesterol in chloroform, add purified water to 100 parts, and rotate under reduced pressure at 70 rpm to evaporate the organic solvent to form a uniform lipid film;

[0067] After mixing the solution containing 0.005 parts of the complex biologically active factor with the uniform lipid film, the membrane is hydrated and removed by rotating at 20°C and 40 rpm to form the crude liposome of the complex biologically active factor;

[0068] The obtained composite bioactive factor crude liposome is granulated into a composite bioactive factor liposome with a particle diameter of 50 nm.

[0069] In conclusion, the encapsulation efficiency of the obtained composite bioactive factor liposome is 32%, and the activity of more than 64% of the bioactive factors in the composite bioactive factor solution is kept, mainly EGF, BFGF, VEGF, IGF1, IGF2, TGF-B1 , TGF-B2, PDGF and KGF, after being stored at 2-8°C fo...

Embodiment 2

[0071] Dissolve 1 part of HSPC, 1 part of soybean lecithin and 0.1 part of cholesterol in ethanol, add purified water to 100 parts, and rotate under reduced pressure at 100 rpm to evaporate the organic solvent to form a uniform lipid film;

[0072] After mixing the solution containing 0.01 part of the complex bioactive factor with the uniform lipid film, the membrane is hydrated and removed by rotating at 25° C. and 70 rpm to form a thick liposome of the complex bioactive factor;

[0073] The obtained compound bioactive factor crude liposome is granulated into a compound bioactive factor liposome with a particle diameter of 100 nm.

[0074] In conclusion, the encapsulation efficiency of the obtained composite bioactive factor liposome is 46%, and the activity of more than 70% of the bioactive factors in the composite bioactive factor solution is kept, mainly EGF, BFGF, VEGF, IGF1, IGF2, TGF-B1 , TGF-B2, PDGF and KGF, after being stored at 2-8°C for 6 months, the encapsulation ...

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Abstract

The invention relates to the field of biology, and especially relates to a composite bioactive factor liposome and a preparation method thereof. The preparation method can improve the bioactivity and the stability of the composite bioactive factor liposome, and the composite bioactive factor liposome can be refrigerated for a long time. The composite bioactive factor liposome provided in the embodiment of the invention comprises 1-5 parts of phospholipid, 0.02-0.2 parts of cholesterol, 0.005-0.01 parts of a composite bioactive factor, and the balance of purified water, wherein the phospholipid and the cholesterol form a liposome, and parts of the composite bioactive factor is encapsulated in the liposome to form the composite bioactive factor liposome.

Description

technical field [0001] The invention relates to the field of biology, in particular to a composite bioactive factor liposome and a preparation method thereof. Background technique [0002] Bioactive factors are produced by various cell types through paracrine, autocrine or endocrine methods, and can regulate cell growth, differentiation and immune functions, especially can effectively interact with skin cells, participate in inflammation and wound healing . For example: bioactive factors can promote the nutrient metabolism of epithelial cells and prevent the skin from being damaged by ultraviolet rays and free radicals; bioactive factors can also promote the proliferation of collagen cells in the dermis and accelerate postoperative skin repair. [0003] Common bioactive factors secreted by cells include EGF (Chinese: epidermal growth factor, English: epidermal growth factor), BFGF (Chinese: basic fibroblast growth factor, English: basicfibroblast growth factor), VEGF (Chine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07A61K9/127A61K38/18A61K38/30A61K8/64A61K8/14
Inventor 南艳萍张青刘莹张琪张爱兵
Owner 陕西瑞妍化妆品有限公司
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