Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting glucosamine from corn dregs by biotransformation

A glucosamine and biotransformation technology, applied in the field of biochemical industry, can solve the problem of not realizing high-value processing, and achieve the effect of realizing resource utilization and high-value utilization, relieving bone and joint pain, improving technical level and comprehensive benefits

Active Publication Date: 2019-12-06
YANGZHOU RIXING BIO TECH +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in recent years, a large number of industrial edible fungi have been built in various parts of the country. The discarded mycorrhizae and fungal residues are also good raw materials for the production of ammonia sugar. At present, these wastes have basically not been processed with high value, and most of them are returned to the field. or burn directly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Add 2 parts of ammonium sulfate, 1 part of potassium phosphate, 0.5 parts of sorbitan tristearate, and 50 parts of water to 12 parts of corn dregs, heat to 100°C for 15 minutes, then cool to 25°C and add concentration 5×10 8 / mL Mucor viridans spores, mix the material evenly and carry out shallow plate fermentation, the fermentation time is 48h, then vacuumize, the vacuum degree is -0.2 MPa, lower the temperature to -10°C, and place it for 24h; then the ultrasonic power is 60W , under the condition that the ultrasonic temperature is 40° C. for 20 min, and separated to obtain glucosamine with a purity of 88%.

Embodiment 2

[0018] Add 3 parts of ammonium sulfate, 2 parts of potassium phosphate, 1 part of sorbitan tristearate, and 60 parts of water to 20 parts of corn dregs, heat to 120°C for 20 minutes, then cool to 30°C and insert The concentration is 5×10 8 / mL Mucor viridans spores, mix the material evenly and carry out shallow plate fermentation, the fermentation time is 50h, then vacuumize, the vacuum degree is -0.1 MPa, lower the temperature to -5°C, and place it for 30h; then the ultrasonic power is 60W , carried out ultrasonic extraction for 30 min under the condition of ultrasonic temperature of 40° C., and separated to obtain glucosamine with a purity of 87%.

Embodiment 3

[0020] Add 2 parts of ammonium sulfate, 1 part of potassium phosphate, 0.5 parts of sorbitan tristearate, and 50 parts of water to 12 parts of corn dregs, heat to 100°C for 15 minutes, then cool to 25°C and add concentration 5×10 8 / mL Mucor viridis spores, mix the material evenly and carry out shallow plate fermentation, the fermentation time is 48h, then vacuumize, the vacuum degree is -0.2 MPa, lower the temperature to -10°C, and place it for 24h; the added concentration is 0.1~0.2 1 to 2 parts of mol / L acetic acid, stirred for 30 minutes, and then ultrasonically extracted for 20 minutes under the conditions of ultrasonic power of 60W and ultrasonic temperature of 40°C, separated to obtain glucosamine with a purity of 92%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting glucosamine from corn grits through bioconversion. The method is conducted according to the following steps that 2-3 parts of ammonium sulfate, 1-2 parts of potassium phosphate, 0.5-1 part of sorbitan tristearate and 50-60 parts of water are added into 10-12 parts of the corn grits, the mixture is heated to 100 DEG C-120 DEG C, the temperature is kept for 15-20 min, then the mixture is cooled to 25 DEG C-30 DEG C, inoculated with green mucor spores and subjected to shallow tray fermentation for 48-50 h after being stirred uniformly, vacuumizing is conducted, the temperature is lowered, and the product is placed for 24-30 h; glucosamine is obtained after ultrasonic extraction and separation are conducted. The bottleneck problems that in the prior art, a raw material is low in chitin content, extraction efficiency is low, and refining process is difficult are solved, the technological level of the glucosamine industry is promoted, and comprehensive benefits of the glucosamine industry are increased.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for extracting glucosamine from corn dregs through biotransformation. Background technique [0002] Glucosamine, 2-amino-2-deoxy-D-glucose, referred to as glucosamine, is a dietary supplement for osteoarthritis and an adjuvant drug for clinical treatment with important application value. It can strengthen cartilage structure and prevent joint disease , Repair cartilage damage caused by osteoporosis, relieve pain, stiffness and swelling caused by arthritis. With the aging trend of the world's population structure, there are more and more patients with osteoarthritis. As a dietary supplement, glucosamine is widely used in the prevention and auxiliary treatment of osteoarthritis. The global market of glucosamine has grown to 30,000 tons , the output value of related products is about 4 billion US dollars. [0003] With the rapid development of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12R1/785
CPCC12P19/26
Inventor 张超史劲松丁振中冯小海柳志强孙达锋胡忠策谢志刚李鹏龚劲松张晓娟方祥赵耀
Owner YANGZHOU RIXING BIO TECH