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Pyocyanine culture medium and method for detecting Pseudomonas aeruginosa in cosmetics

A technology of Pseudomonas aeruginosa and pyocyanin, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve the problems of cumbersome steps and long detection time

Active Publication Date: 2016-05-25
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of tedious steps and long detection time in the detection method of Pseudomonas aeruginosa in the prior art, and provide a simple, fast and efficient method for detecting Pseudomonas aeruginosa in cosmetics Pyocyanin medium and method

Method used

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  • Pyocyanine culture medium and method for detecting Pseudomonas aeruginosa in cosmetics
  • Pyocyanine culture medium and method for detecting Pseudomonas aeruginosa in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Detection method of Pseudomonas aeruginosa in toner

[0023] Preparation of pyocyanin medium: firstly dissolve lecithin 1.2g and Tween-806.0g with distilled water, then add peptone 20.0g, magnesium chloride 1.4g, potassium sulfate 10.0g, triclosan (2,4,4 '-trichloro-2'-hydroxydiphenyl ether) 0.05g, gelatin 30.0g and agar 13.0g, add water to 1L, stir and mix, adjust the pH value to 6.9, and autoclave at 121°C for 20min.

[0024] Detection method:

[0025] (1) Bacterial enrichment culture: Aseptically weigh 10g of toner and add it into a glass bottle containing 90mL of SCDLP bacterium enrichment solution, and mix well to prepare a cosmetic sample preparation solution; add 100mL of SCDLP bacterium enrichment solution into another glass bottle, Prepare a blank control solution; add 10 mL of 100 cfu / mL Pseudomonas aeruginosa standard strain ATCC9027 bacteria solution into another glass bottle containing 90 mL of SCDLP enrichment solution, and mix well to prepare ...

Embodiment 2

[0029] Embodiment 2: the detection method of Pseudomonas aeruginosa in facial cleanser

[0030] Preparation of pyocyanin medium: firstly dissolve 1.0g lecithin and Tween-808.0g with distilled water, then add 18.0g peptone, 1.6g magnesium chloride, 8.0g potassium sulfate, triclosan (2,4,4 '-trichloro-2'-hydroxydiphenyl ether) 0.1g, gelatin 28.0g and agar 15.0g, add water to 1L, stir and mix, adjust the pH value to 7.0, and autoclave at 121°C for 20min.

[0031] Detection method:

[0032] (1) Bacterial enrichment culture: Aseptically weigh 10g of facial cleanser and add it to a glass bottle containing 90mL of SCDLP enrichment solution, and mix well to prepare a test solution for cosmetic samples; add 100mL of SCDLP bacteria enrichment solution into another glass bottle 10mL400cfu / mL Pseudomonas aeruginosa standard strain ATCC9027 bacteria solution was added to another glass bottle containing 90mL SCDLP enrichment solution, and mixed well to prepare a positive control solution. T...

Embodiment 3

[0036] Embodiment 3: the detection method of Pseudomonas aeruginosa in liquid foundation

[0037] Preparation of pyocyanin medium: first lecithin 0.8g and Tween-807.0g were heated and dissolved with distilled water, then respectively added peptone 18.0g, magnesium chloride 1.4g, potassium sulfate 12.0g, triclosan (2,4,4 '-trichloro-2'-hydroxydiphenyl ether) 0.1g, gelatin 25.0g and agar 13.0g, add water to 1L, stir and mix, adjust the pH value to 7.1, and autoclave at 121°C for 20min.

[0038] Detection method:

[0039] (1) Bacterial enrichment culture: Aseptically weigh 10g of foundation liquid and add it to a glass bottle containing 80mL of SCDLP bacterial enrichment solution, add 10mL of Tween-80, and mix well to prepare a cosmetic sample preparation solution; 100mL of SCDLP bacterial enrichment solution into another glass bottle to prepare a blank control solution; add 10mL of 700cfu / mL Pseudomonas aeruginosa standard strain ATCC9027 bacteria solution into another glass bo...

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Abstract

The invention discloses a pyocyanine culture medium and method for detecting Pseudomonas aeruginosa in cosmetics. Every liter of the pyocyanine culture medium contains 0.8-1.2g of lecithin, 6.0-8.0g of Tween-80, 18.0-20.0g of peptone, 1.4-1.6g of magnesium chloride, 8.0-16.0g of potassium sulfate, 0.05-0.1g of triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether), 25.0-30.0g of gelatin, 13.0-15.0g of agar and the balance of water. The pH value is 6.9-7.1. The method comprises the following steps: carrying out enrichment culture on a cosmetic sample by using an SCDLP (soya casein digest lecithin polysorbate broth) enrichment fluid, directly carrying out a 42-DEG C growth test by using the pyocyanine culture medium, carrying out screening detection on Pseudomonas aeruginosa, observing the screening culture result, and if bacterial colonies appear after the screening culture, further carrying out identification in combination with a gelatin liquefaction test. The method simplifies the operation steps in the Pseudomonas aeruginosa detection process, enhances the detection efficiency, and can be used for daily detection on Pseudomonas aeruginosa in cosmetics.

Description

technical field [0001] The invention belongs to the field of cosmetic microbial safety detection, and in particular relates to a pyocyanin culture medium and a method for detecting Pseudomonas aeruginosa in cosmetics. Background technique [0002] Pseudomonas aeruginosa (Pseudomonas aeruginosa) is widely distributed in water, air, soil and the surface of objects in nature, and can also be found in the body surface and intestinal tract of normal humans and animals. Gram-negative bacilli that are resistant to a variety of antibiotics can infect the human body through contact with damaged skin and mucous membranes. They are important opportunistic pathogens that can cause pneumonia and sepsis in patients in severe cases. In recent years, cosmetics have increasingly become a necessity in people's daily life. The nutrients in cosmetics (such as protein, oil, vitamins, etc.) are conducive to the growth and metabolism of Pseudomonas aeruginosa, and their metabolites can decompose s...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/045
Inventor 文霞周少璐杨秀茳孙廷丽谢小保
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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