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A preparation method of Cordyceps militaris extract rich in biologically active substances

A bioactive substance, the technology of Cordyceps militaris, which is applied in the field of preparation of Cordyceps militaris extract rich in bioactive substances, can solve the problems of low application value, single active ingredient, and low content, and achieve the effect of good development and application prospects

Active Publication Date: 2018-11-27
李学龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, according to public literature reports at home and abroad, the preparation methods of Cordyceps militaris extract mainly include the method of boiling the fruiting body of Cordyceps militaris, boiling and alcohol precipitation, alcohol extraction, supercritical CO 2 Extraction method, resin adsorption method, etc., or use Cordyceps militaris medium water to mention organic solvent extraction, or use amylase to hydrolyze the Cordyceps militaris medium and then extract, the active ingredients such as cordycepin and cordyceps polysaccharide in the Cordyceps militaris extract are relatively single, and The content is not high, the extraction rate is low
Because in the process of using these methods to prepare Cordyceps militaris extracts, non-food-grade chemical reagents such as chloroform, n-butanol, and organic entraining agents will be introduced, which are difficult to completely remove during subsequent processing, which brings great hidden dangers to food safety. , so the practical application value is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing a Cordyceps militaris extract rich in biologically active substances, the specific steps are as follows:

[0025] (1), preparation of Cordyceps militaris compound enzyme system solution: put 10kg of pure cultured Cordyceps militaris mycelium matrix into the stirring extraction tank, add 5 times of pure water by weight, extraction temperature 25°C, stirring speed 80r / min , extraction time is 2 hours, filter with 10 μm pore size membrane, add 3 times volume of 95% edible ethanol to the filtrate, cool down to 10°C, and centrifuge at 12000r / min to obtain a compound enzyme precipitate, and use 12 times volume to pH 4.0 Food-grade acetic acid / sodium acetate buffer solution is dissolved to obtain the Cordyceps militaris compound enzyme solution solution;

[0026] (2) Hydrolysis reaction of Cordyceps militaris compound enzyme system: put 100kg of purified Cordyceps militaris fruiting bodies into a forced external circulation extraction tank, add pure wate...

Embodiment 2

[0032](1), the preparation of Cordyceps militaris compound enzyme system solution: 10Kg of the Cordyceps militaris mycelium substrate of 10kg pure culture is dropped in the stirring extraction tank, add 10 times of pure water by weight ratio, extraction temperature 45 ℃, stirring speed 120r / / Min, extraction time 4h, filter with 10μm pore size membrane, add 5 times the volume of 95% edible ethanol to the filtrate, cool to 8°C, centrifuge at 18000r / min to obtain the enzyme precipitate, use 8 times the volume to pH 6.0 Food-grade acetic acid / sodium acetate buffer solution is dissolved to obtain the Cordyceps militaris compound enzyme system solution;

[0033] (2) Hydrolysis reaction of Cordyceps militaris compound enzyme system: put 100kg of purified fruiting bodies of Cordyceps militaris into the forced external circulation extraction tank, add pure water 20 times the weight of the fruiting bodies of Cordyceps militaris, keep the extraction temperature at 45°C, add volume Cordy...

Embodiment 3

[0039] (1), preparation of Cordyceps militaris compound enzyme system solution: put 10kg of pure cultured Cordyceps militaris mycelium matrix into the stirring extraction tank, add 8 times of pure water by weight, extraction temperature 35°C, stirring speed 100r / min , the extraction time is 3 hours, filter with a 10 μm pore size filter, add 4 times the volume of 95% edible ethanol to the filtrate, cool to 6 ° C, and centrifuge with a speed of 16000r / min to obtain the enzyme precipitate, and use 10 times the volume of pH 5.0 The food-grade acetic acid / sodium acetate buffer solution is dissolved to obtain the Cordyceps militaris compound enzyme system solution;

[0040] (2) Hydrolysis reaction of Cordyceps militaris compound enzyme system: Put 100kg of purified fruiting bodies of Cordyceps militaris into the forced external circulation extraction tank, add pure water 16 times the weight of the fruiting bodies of Cordyceps militaris, keep the extraction temperature at 35°C, add vo...

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PUM

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Abstract

The invention relates to a method for extracting cordyceps extract with abundant bioactive substances, and belongs to the field of functional foods. The method includes steps of preparing cordyceps biological transformation compound enzyme systems with biological enzymes such as superoxide dismutase, glycosidase, transferase, hydrolase and isomerase; enabling the ordyceps biological transformation compound enzyme systems to carry out hydrolysis reaction; efficiently, dynamically and circularly extracting the cordyceps bioactive substances; filtering the cordyceps bioactive substances by the aid of microporous filter membranes; concentrating the cordyceps bioactive substances under vacuum conditions to obtain the cordyceps extract with the abundant bioactive substances. Compared with existing disclosed methods for preparing cordyceps extract, the method has the advantages that the contents of the bioactive substances such as cordycepin, cordycepic acid, cordyceps polysaccharides and adenosine in the cordyceps extract can be greatly increased; active substance components in cordyceps are secondarily biologically transformed by the aid of processes for efficiently transforming biological compound enzymes and the like, accordingly, the contents of the bioactive substances in the cordyceps extract are higher than the corresponding contents of cordyceps fruit bodies, and the method has excellent development and application prospects.

Description

technical field [0001] The invention belongs to the field of functional foods, and particularly provides a method for preparing a Cordyceps militaris extract rich in biologically active substances. Background technique [0002] Cordyceps militaris is a precious traditional Chinese medicine and a high-grade tonic. It has a sweet and flat taste, nourishes the lungs and kidneys, nourishes the essence, stops bleeding and resolves phlegm. value and health function. Modern pharmacological studies have shown that the active ingredients of Cordyceps militaris such as cordycepin, cordycepic acid, cordyceps polysaccharide, and adenosine have anti-tumor, hypoglycemic, and immune-enhancing functions. Body immunity and so on. With the acceleration of the pace of life and the transformation of ideas, people pay more and more attention to the health care and conditioning of the body. However, due to over-excavation of wild Cordyceps militaris, wild resources are very scarce. Great deman...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L31/00A23L33/105
Inventor 刘宏非
Owner 李学龙