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Terahertz spectrum technology based urine detection method

A spectrum technology and detection method technology, applied in the field of urine detection, can solve the problems of terahertz measurement of urine protein slowly, lack of absorption characteristic peaks, etc., achieve fast detection speed, low cost, and avoid pollution

Inactive Publication Date: 2016-06-01
CAPITAL NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0007] The development of various foreign research organizations using terahertz to measure urine protein is also very slow. At present, only Cunningham et al. from the University of Leeds have obtained absorption spectra by using mouse urine protein single crystals, but they lack obvious absorption characteristic peaks, so it is difficult to use this method. method to distinguish different cases
Therefore, at present, there is still a lack of rapid and effective spectroscopic methods to distinguish different cases based on the detection of urine protein in urine at home and abroad.

Method used

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  • Terahertz spectrum technology based urine detection method

Examples

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Embodiment 1

[0035] This embodiment provides a method for detecting protein content in urine using terahertz time-domain spectroscopy, which includes the following steps:

[0036] 1) Select the sample cell: the sample cell used is a high-purity quartz sample cell developed by Starna Company in the United Kingdom, with an optical path of 0.5mm.

[0037] 2), source of urine: Patient Jiang, male, 68 years old, suffering from diabetic nephropathy for 2 years, 24 hours urine volume 2780ml, measured 24 hours urine protein quantitative (UPr) biochemical result was 0.

[0038] 3) Obtaining and filling of urine: Obtain fresh urine samples from different patients from the laboratory department of a tertiary hospital in Beijing, then use a pipette gun to extract and inject into the sample pool, cover the lid, and complete the filling.

[0039] 4) Detection of zero protein urine:

[0040] Turn on the laser until the output power is stable, calibrate the light collimation in the optical path; measure ...

Embodiment 2

[0047] This embodiment provides a method for detecting abnormal low-protein urine using terahertz time-domain spectroscopy, and the operation steps are exactly the same as those in Embodiment 1. Selected urine source case information: Patient Liu, female, 48 years old, has suffered from diabetic nephropathy for 6 years, 24-hour urine volume is 2350ml, and the biochemical result of 24-hour urine protein quantitation (UPr) is 0.188. The samples to be tested were cases containing low protein urine. Among them, the terahertz measurement spectrum is shown in image 3 .

Embodiment 3

[0049] This embodiment provides a method for detecting high-protein urine using terahertz time-domain spectroscopy, and the operation steps are exactly the same as those in Embodiment 1. Selected urine source case information: Patient Wang, male, 58 years old, has suffered from diabetic nephropathy for 9 years, 24-hour urine volume is 1700ml, and the biochemical result of 24-hour urine protein quantitation (UPr) is 3.706. The sample to be tested is the case of high proteinuria. Among them, the terahertz measurement spectrum is shown in Figure 4 .

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Abstract

The invention discloses a terahertz spectrum technology based urine detection method. Based on a terahertz spectrum measurement technology, the relative content of proteins in urine of different kidney patients is distinguished by utilizing chromatic dispersion changes of body fluids under the induction of terahertz radiation. According to the method, the problem in physical parameters for measuring urine conditions is solved; the method does not depend on the assistance of a chemical regent, so that external chemical pollution is alleviated and the method is a pure physical detection method; and the method is simple to operate, quick in data processing and accurate in result.

Description

technical field [0001] The invention relates to the field of urine detection, in particular to a method for detecting urine based on terahertz spectrum technology, and a terahertz spectrum analysis method for distinguishing protein content in different urine samples. Background technique [0002] Urine examination is the most extensive examination content in clinical examination work except blood examination. Urine analysis is an important examination item, which has special clinical diagnosis and differential value, and helps doctors to observe the curative effect and judge the prognosis in clinical practice. Urine is also a painless, readily available test sample. Urinalysis has important clinical diagnostic value for various clinical diseases such as urinary system diseases, endocrine system diseases, circulatory system diseases, digestive system diseases, reproductive system diseases, etc. At the same time, urine testing is also an essential testing item for drug monito...

Claims

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Application Information

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IPC IPC(8): G01N21/3586G01N21/3577
Inventor 左剑刘尚建张存林
Owner CAPITAL NORMAL UNIVERSITY
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