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Formula of anther induction medium for cultivation of capsicum annuum variety

A technology for inducing medium and medium formula, applied in the directions of application, horticultural methods, botanical equipment and methods, etc., can solve the gap, the interaction relationship between medium components and culture conditions is unclear, embryoid body or callus problems such as low induction rate and differentiation rate, to achieve the effect of speeding up the cultivation process and improving the cultivation efficiency

Active Publication Date: 2016-06-08
新沂市芭缇雅商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The interaction between medium components and culture conditions is unclear, and the induction rate and differentiation rate of embryoid bodies or callus are low, which is far from the actual application requirements.

Method used

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  • Formula of anther induction medium for cultivation of capsicum annuum variety
  • Formula of anther induction medium for cultivation of capsicum annuum variety
  • Formula of anther induction medium for cultivation of capsicum annuum variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Prepare the culture medium with the following formula:

[0019] KNO 3 950mg / L, NH 4 NO3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ?H 2 O1200mg / L, CaCl 2 ?2H 2 O225mg / L, Na 2 -EDTA75mg / L, FeSO 4 ?7H 2 O55mg / L, MnSO 4 ?H 2 O17mg / L, ZnSO 4 ?7H 2 O18mg / L, H 3 BO 3 6.0mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, CoCl? 6H 2 O0.03mg / L, NaMoO 4 ?2H 2 O0.25mg / L, AgNO 3 5.0mg / L, inositol 100mg / L, vitamin B10.5mg / L, vitamin B60.5mg / L, niacin 5.0mg / L, glycine 2.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3g / L, maltose 20g / L, sucrose 15g / L, vegetable gel 5.0g / L;

[0020] 2,4-D 0.6mg / L, kinetin 0.5mg / L, complex nucleic acid 0.8mg / L, biotin 0.05mg / L, vitamin C 45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.4g / L, pH5 .8.

[0021] Pepper cultivars Xinfeng No. 5 and Chaotianjiao were selected as donors for anther culture. On sunny mornings when the temperature was low and the sunshine was weak, flower buds without pests and diseases (calyx and pet...

Embodiment 2

[0024] Prepare the medium with the following formula (AgNO 3 Adding the preferred concentration):

[0025] KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ?H 2 O1200mg / L, CaCl 2 ?2H 2 O225mg / L, Na 2 -EDTA75mg / L, FeSO 4 ?7H 2 O55mg / L, MnSO 4 ?H 2 O17mg / L, ZnSO 4 ?7H 2 O18mg / L, H 3 BO 3 6.0mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, CoCl? 6H 2 O0.03mg / L, NaMoO 4 ?2H 2 O0.25mg / L, inositol 100mg / L, vitamin B10.5mg / L, vitamin B60.5mg / L, niacin 5.0mg / L, glycine 2.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3 g / L, maltose 20g / L, sucrose 15g / L, vegetable gel 5.0g / L;

[0026] 2,4-D 0.6mg / L, kinetin 0.5mg / L, complex nucleic acid 0.8mg / L, biotin 0.05mg / L, vitamin C 45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.4g / L, pH5 .8.

[0027] AgNO 3 The added concentration is set to the following 8 types: 0; 1.0mg / L; 2.0mg / L; 3.0mg / L; 4.0mg / L; 6.0mg / L; 7.0mg / L; 8.0mg / L.

[0028] The two pepper varieties were also selected as anther do...

Embodiment 3

[0030] Prepare the medium with the following formula (the preferred concentration of glycine added):

[0031] KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ?H 2 O1200mg / L, CaCl 2 ?2H 2 O225mg / L, Na 2 -EDTA75mg / L, FeSO 4 ?7H 2 O55mg / L, MnSO 4 ?H 2 O17mg / L, ZnSO 4 ?7H 2 O18mg / L, H 3 BO 3 6.0mg / L, KI0.8mg / L, CuSO 4 ?5H 2 O0.025mg / L, CoCl? 6H 2 O0.03mg / L, NaMoO 4 ?2H 2 O0.25mg / L, AgNO 3 5.0mg / L, inositol 100mg / L, vitamin B10.5mg / L, vitamin B60.5mg / L, niacin 5.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3g / L, maltose 20g / L L, sucrose 15g / L, plant gel 5.0g / L;

[0032] 2,4-D 0.6mg / L, kinetin 0.5mg / L, complex nucleic acid 0.8mg / L, biotin 0.05mg / L, vitamin C 45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.4g / L, pH5 .8.

[0033] The added concentration of glycine is set in the following four types: 0; 1.0mg / L; 3.0mg / L; 4.0mg / L.

[0034] The two pepper varieties were also selected as anther donors, the operation method and ...

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Abstract

The invention provides a formula of an anther induction medium for cultivation of the capsicum annuum variety. The formula of the medium comprises KNO3, NH4NO3, KH2PO4, MgSO4*H2O, CaCl2*2H2O, Na2-EDTA (ethylene diamine tetraacetic acid), FeSO4*7H2O, MnSO4*H2O, ZnSO4*7H2O, H3BO3, KI, CuSO4*5H2O, CoCl*6H2O, NaMoO4*2H2O, AgNO3, inositol, vitamin B1, vitamin B6, nicotinic acid, glycine, folic acid, cyclic guanosine monophosphate, maltose, cane sugar, phytagel, 2,4-D, kinetin, polyphthalein nucleic acid, biotin, vitamin C, activated carbon and hydrolyzed protein. The medium has the advantages that capsicum annuum anther is efficiently induced to produce pollen callus, the capsicum annuum anther cultivation efficiency can be remarkably improved, and the fine variety cultivation process of capsicum annuum is accelerated.

Description

technical field [0001] The invention relates to an anther-inducing medium formula for cultivating pepper varieties, in particular to an induction medium formula for significantly improving the anther-curing rate of pepper, and belongs to the field of agricultural science and technology. Background technique [0002] Pepper (Capsicumannuum L.), also known as pepper, sea pepper, green pepper, pepper, etc., is a vegetable crop of Solanaceae, an important economic crop, and a vegetable crop commonly cultivated in the world. Capsicum peel and placenta tissue are rich in carotene and vitamin C, especially the Vc content in pepper is the highest among vegetables, so the nutritional value is extremely high. Capsaicin contained in chili has the functions of enhancing appetite, helping digestion, enhancing human body resistance, and resisting various diseases. Capsicum has a high yield, a long supply season, various ways of eating, and rich nutrition. It is a vegetable that is deeply...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张瑞明
Owner 新沂市芭缇雅商贸有限公司
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