Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Flow shear stress mediated collagen self-assembly method

A fluid shear force, self-assembly technology, applied in the field of biomedical materials and biomechanics, can solve the problems of not being able to reproduce the assembly process well, ignoring the mechanical microenvironment, etc., achieving easy recovery and processing, simple preparation method, The effect of good biocompatibility and degradability

Active Publication Date: 2016-06-08
SHENZHEN LANDO BIOMATERIALS
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional induction method ignores the mechanical microenvironment, so it cannot reproduce the in vivo assembly process very well.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Flow shear stress mediated collagen self-assembly method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Collagen self-assembly mediated by fluid shear force

[0025] Add 400 μL of 10xPBS solution, 2.76 μL of 1M NaOH solution, and 3.477 mL of deionized water to the centrifuge tube in sequence, mix well, and take out the type I rat tail collagen solution with a mass concentration of 3.34 mg / mL from the refrigerator at 4 °C. Take 119.8 μL and add it to prepare a sample solution with a mass concentration of 0.10 mg / mL, and adjust the pH between 7.0 and 7.4 with diluted ammonia water. Take 3.9mL of the sample solution and drop it on the plate of the cone-plate viscometer, put down the cone-plate, set the fluid shear force to 0.5Pa, the action time is 1h, the reaction temperature is 25°C, and the force is applied. After the stress loading was completed, the suspension was collected, centrifuged at high speed and washed 3 times. The precipitate was frozen in a -20°C refrigerator for 5 hours, and then transferred to a vacuum freeze dryer for 12 hours to freeze-dry to ...

Embodiment 2

[0026] Example 2: Collagen self-assembly mediated by fluid shear force

[0027] Add 400 μL of 10xPBS solution, 2.76 μL of KOH solution with a molar concentration of 1M, and 3.477 mL of deionized water in sequence in the centrifuge tube, mix well, and take out the type I rat tail collagen solution with a mass concentration of 3.34 mg / mL from the refrigerator at 4 °C. Take 119.8 μL and add it to prepare a sample solution with a mass concentration of 0.10 mg / mL, and adjust the pH between 7.0 and 7.4 with diluted sodium hydroxide solution. Take 3.9mL of the sample solution and drop it on the plate of the cone-plate viscometer, put down the cone-plate, set the fluid shear force to 0.5Pa, the action time is 1h, the reaction temperature is 25°C, and the force is applied. After the stress loading was completed, the suspension was collected, centrifuged at high speed and washed 3 times. The precipitate was frozen in a -20°C refrigerator for 5 hours, and then transferred to a vacuum fr...

Embodiment 3

[0028] Example 3: Collagen self-assembly mediated by fluid shear force

[0029] Add 400 μL of 10xPBS solution, 2.76 μL of 1M NaOH solution, and 3.477 mL of deionized water to the centrifuge tube in sequence, mix well, and take out the type I rat tail collagen solution with a mass concentration of 3.34 mg / mL from the refrigerator at 4 °C. Take 119.8 μL and add it to prepare a sample solution with a mass concentration of 0.10 mg / mL, and adjust the pH between 7.0 and 7.4 with diluted ammonia water. Take 3.9mL of the sample solution and drop it on the plate of the cone-plate viscometer, put down the cone-plate, set the fluid shear force to 2.0Pa, the action time is 1h, the reaction temperature is 25°C, and the force is applied. After the stress loading was completed, the suspension was collected, centrifuged at high speed and washed 3 times. The precipitate was frozen in a -20°C refrigerator for 5 hours, and then transferred to a vacuum freeze dryer for 12 hours to freeze-dry to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Fiber diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a flow shear stress mediated collagen self-assembly method, and belongs to the field of biomedical materials and biomechanics. According to the method, a self-assembly mechanism of collagen is started under a neutral condition, and a flow shear action in a constant direction is applied to the collagen by utilizing a cone-and-plate viscometer and other mechanical loading devices. Compared with a traditional self-assembly method, the method has the advantages that a mechanic-chemical coupling system is constructed by taking the biomechanic microenvironment into consideration, and a real assembling process in a body is better simulated. Under mediation of a fluid shear stress, collagenous fibers are rearranged, so that subsequent deposition of hydroxyapatite can be facilitated. The novel collagenous fiber material has good biocompatability and degradability, has a mechanical characteristic matched with the collagen of human bone tissues, and has a wide application space.

Description

technical field [0001] The invention belongs to the fields of biomedical materials and biomechanics. More specifically, the present invention relates to a method for fluid shear mediated collagen self-assembly. Background technique [0002] Due to the increasing number of cases of bone defects caused by factors such as trauma, strain, population aging, and congenital deformities, the development and application of bone repair materials have attracted increasing attention. The traditional method uses autologous bone or allogeneic bone graft, which is expensive and often accompanied by complications. By simulating the composition and structure of natural bone, the developed bionic bone repair materials provide new ideas and strategies for this purpose. Natural bone is a collagen-calcium phosphate composite system with a complex hierarchical structure, the organic phase imparts toughness to the backbone, and the inorganic phase imparts strength to the backbone. Collagen acco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/24A61L27/50A61L27/58
CPCA61L27/24A61L27/50A61L27/58A61L2430/02C08L89/04
Inventor 牛旭锋郭晓霖樊瑜波李萍范睿
Owner SHENZHEN LANDO BIOMATERIALS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com