Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns

A technology of immune enhancer and screening method, which is applied in the field of rapid screening of compound Chinese herbal medicine immune enhancer, which can solve the problems of long experimental period, unknown content of active ingredients, easy to be affected by external factors, etc., and achieve low detection cost and accurate immunity Enhanced effect

Inactive Publication Date: 2016-06-08
OCEAN UNIV OF CHINA
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of compound Chinese herbal medicine has the problems of large amount of addition, unclear content of active ingredients, and unclear mechanism of action.
In addition, the traditional method of screening compound Chinese herbal medicines is to use breeding experiments. The experimental period is long, and it is easily affected by external factors. It is impossible to screen out a suitable compound Chinese herbal medicine immune enhancer in a short time.
And the traditional repeated screening method consumes a lot of time, manpower, material resources and financial resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns
  • Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns
  • Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] In this embodiment, Litopenaeus vannamei is selected as the test object, and Litopenaeus vannamei is the main species of domestic prawn farming at present. Four Chinese herbal medicines, Astragalus, Coptidis Rhizoma, Eucommia and Rhubarb, were selected and combined as immune enhancers for rapid screening.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rapid screening method of a compound Chinese-herbal-medicine immunopotentiator for prawns. The rapid screening method includes the steps of 1, preparing of a prawn-muscle extracting solution; 2, preparing of a blood-cell medium; 3, prawn-blood-cell primary culturing; 4, extracting of Chinese-herbal-medicine effective components; 5, measuring of the survival rate, phagocytic activity, antibacterial activity and bacteriolytic activity. According to the rapid screening method, as the combination of multiple Chinese-herbal-medicine extracts is added into a prawn-blood-cell primary culturing system, and the combination with the good immuno-enhancement effect can be rapidly screened. The rapid screening method has the advantages of being rapid, efficient, accurate, stable and the like; as cell culturing is adopted, compared with the traditional breeding mode, detection cost is lower.

Description

technical field [0001] The invention relates to a rapid screening method for a compound Chinese herbal medicine immune enhancer, in particular to a rapid screening method for combining a Chinese herbal immune enhancer by using primary culture of prawn blood cells, and belongs to the technical field of aquaculture and biological screening. Background technique [0002] In recent years, due to the degradation of germplasm resources and the deterioration of the breeding environment, frequent outbreaks of shrimp diseases have severely restricted the development of shrimp farming. The traditional way to prevent and treat diseases is to use antibiotics. However, the widespread use of antibiotics will lead to problems such as drug residues, bacterial strain resistance, and environmental pollution, which will eventually endanger human health. Therefore many scholars research the alternatives of antibiotics. As a green natural medicine, Chinese herbal medicine has the advantages of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/02
CPCG01N33/502G01N33/5044
Inventor 潘鲁青高国瑞黄辉
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com