Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns

A technology of immune enhancer and screening method, which is applied in the field of rapid screening of compound Chinese herbal medicine immune enhancer, which can solve the problems of long experimental period, unknown content of active ingredients, easy to be affected by external factors, etc., and achieve low detection cost and accurate immunity Enhanced effect

Inactive Publication Date: 2016-06-08
OCEAN UNIV OF CHINA
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Problems solved by technology

However, the use of compound Chinese herbal medicine has the problems of large amount of addition, unclear content of active ingredients, and unclear mechanism of action.
In addition, the traditional method of screening compound Chinese herbal medicines is to use breeding experiments. The experime

Method used

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  • Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns
  • Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns
  • Rapid screening method of compound Chinese-herbal-medicine immunopotentiator for prawns

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Example Embodiment

[0022] Examples:

[0023] In this embodiment, Litopenaeus vannamei is selected as the test object, and Litopenaeus vannamei is currently the main species of shrimp farming in China. Four Chinese herbal medicines: Astragalus, Rhizoma Coptidis, Eucommia and Rhubarb were selected as a rapid screening immune enhancer.

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Abstract

The invention relates to a rapid screening method of a compound Chinese-herbal-medicine immunopotentiator for prawns. The rapid screening method includes the steps of 1, preparing of a prawn-muscle extracting solution; 2, preparing of a blood-cell medium; 3, prawn-blood-cell primary culturing; 4, extracting of Chinese-herbal-medicine effective components; 5, measuring of the survival rate, phagocytic activity, antibacterial activity and bacteriolytic activity. According to the rapid screening method, as the combination of multiple Chinese-herbal-medicine extracts is added into a prawn-blood-cell primary culturing system, and the combination with the good immuno-enhancement effect can be rapidly screened. The rapid screening method has the advantages of being rapid, efficient, accurate, stable and the like; as cell culturing is adopted, compared with the traditional breeding mode, detection cost is lower.

Description

technical field [0001] The invention relates to a rapid screening method for a compound Chinese herbal medicine immune enhancer, in particular to a rapid screening method for combining a Chinese herbal immune enhancer by using primary culture of prawn blood cells, and belongs to the technical field of aquaculture and biological screening. Background technique [0002] In recent years, due to the degradation of germplasm resources and the deterioration of the breeding environment, frequent outbreaks of shrimp diseases have severely restricted the development of shrimp farming. The traditional way to prevent and treat diseases is to use antibiotics. However, the widespread use of antibiotics will lead to problems such as drug residues, bacterial strain resistance, and environmental pollution, which will eventually endanger human health. Therefore many scholars research the alternatives of antibiotics. As a green natural medicine, Chinese herbal medicine has the advantages of ...

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/502G01N33/5044
Inventor 潘鲁青高国瑞黄辉
Owner OCEAN UNIV OF CHINA
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