Allergy treatment using acid treated aqueous whey protein extract
A whey protein and extract technology, applied in the field of whey protein extract manufacturing, can solve the problem of cytokine deficiency and the like
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example 1
[0195] Example 1 Whey protein including acid-treated aqueous solution containing soluble whey protein components Production of extracts
[0196] Put 200mlH 2 O was added to 12 g of WPC80 and incubated at room temperature for 30 min until dissolved. The solution was centrifuged at 16000 g for 10 min to remove insoluble solids. The supernatant was removed and acidified by adding 1.335 ml of 10 N HCl for 30 min. The acidified solution was neutralized by adding 1.555 ml of 10M NaOH, and the pH was measured, the pH was about 7.0. The final solution was filtered through a 0.45 μm filter, and then passed through a 0.2 μm filter for sterilization. The 6% solution was used as the stock solution for the test (ie, the stock solution was diluted 1 / 8 or 1 / 12 into the formula).
example 2
[0197] Example 2 In vitro immunomodulation of acid-treated aqueous solutions containing soluble whey protein components active
[0198] In a number of cell-based assays, the in vitro immunomodulatory activity of the extract (123) formed in Example 1 was tested. The extract was aliquoted into 96-well plates and then screened in a rapid cell-based screening assay.
[0199] figure 1 The data in shows in vitro TNFα (a Th1 cytokine) released by RAW cells of a macrophage cell line. I23 stimulates TNF-α production in RAW cells (see figure 1 Part number in 3).
[0200] Depending on the surrounding cytokine environment, IL-18 is the main early immunoregulatory cytokine that drives the development of T cells into Th1 (inflammation) or Th2 (allergic) direction. We also assessed IL-18 secretion after stimulation of THP1 cells (a monocyte cell line) with phorbol 12-myristate 13-acetate with or without lipopolysaccharide (LPS). Control cells were in phosphate buffered saline (PBS). Part of I...
example 3
[0208] Example 3 In vivo immune regulation of acid-treated aqueous solutions containing soluble whey protein components active
[0209] In vivo experiments were performed using brown Norwegian allergic juvenile rats. Give acid-activated I23 as a continuous infusion mixed in the formulation or as a bolus of 0.1 ml given to the stomach three times a day. The test was completed with n=8 / group and serum analysis was performed.
[0210] I23 has immunomodulatory activity when used as a supplement into the formula (1 / 8 of the 6% starting solution), or when administered as a bolus to infant rats fed with the formula 3 times / day. When compared to the IgE levels in young rats that received only the formula, a significant reduction in IgE was obtained. A one-way analysis of variance (OneWayANOVA) with Tukey’s post-hoctest was used to evaluate the significance between the groups, *=Compared with the formula feeding group, the treatment group P <0.05.
[0211] Compared with the formula-fed ...
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