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Modified oligochitosan for aquaculture and preparation method thereof

A technology of chitosan oligosaccharide and aquaculture, which is applied in the direction of chemicals, biocides, and disinfectants for biological control, and can solve the problems of low antibacterial activity, no inhibitory activity, and weakened inhibitory effect

Active Publication Date: 2016-06-15
宁波科瑞特动物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, compared with the existing chemically synthesized antibacterial agents, the antibacterial activity is still low and has no inhibitory activity to fungi. In addition, the inhibitory effect of chitosan is also weakened due to the increase of molecular weight, which is the same as the molecular weight of chitosan. Larger, the poorer the water solubility, resulting in weakened antibacterial

Method used

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  • Modified oligochitosan for aquaculture and preparation method thereof
  • Modified oligochitosan for aquaculture and preparation method thereof
  • Modified oligochitosan for aquaculture and preparation method thereof

Examples

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preparation example Construction

[0085] Another aspect of the present invention provides a method for preparing modified chitosan, comprising the following steps:

[0086] (1) immersing chitosan oligomers with a weight-average molecular weight of 3,000 to 50,000 in 2V2vt% acetic acid solution and 2V5wt% hydrogen peroxide solution by weight;

[0087] (2) dissolving piperazine diketonic acid in 10V methanol, and then adding it to the chitosan oligomer solution obtained in the step (1) to obtain a reaction solution;

[0088] (3) adjusting the pH of the reaction solution obtained in the step (2) to be neutral with 10wt% NaOH aqueous solution and filtering, and soaking, washing, filtering and drying the retentate;

[0089] (4) will respectively with In solvent acetonitrile (6V) reaction, the solvent is removed under reduced pressure to obtain quaternary ammonium salt;

[0090] (5) Disperse the oligomeric chitosan piperazine diketoamide synthesized in the step (3) in acetone, add a 20wt% NaOH aqueous solut...

Embodiment 1

[0095] (1) Grafting piperazine diketoacid on chitosan oligomer

[0096] In a 100ml three-necked bottle, add 5g chitosan oligomer with a weight average molecular weight of 20,000, 10ml 2vt% acetic acid solution, and 10ml hydrogen peroxide solution with a mass concentration of 5%, stir, and keep it at 55°C for 5h after it is completely dissolved. ; After the reaction, add the piperazine diketoacid A (0.015g) dissolved in 2ml methanol;

[0097] The reaction was stirred at room temperature for 12 hours to obtain a reaction solution; the pH of the reaction solution was adjusted to neutrality with 10wt% NaOH aqueous solution, filtered, soaked in 20ml of acetone for 10h, washed with 5ml of acetone, filtered, and dried under reduced pressure at 40°C for 12h.

[0098] (2) Graft quaternary ammonium salt on chitosan oligomeric modified with piperazinone diacid

[0099] In a 100ml three-necked flask, add 0.1mol 0.1mol and solvent 50ml of acetonitrile, after 8 hours of reaction at 50°...

Embodiment 2

[0102] The preparation method is the same as that in Example 1, except that the quaternary ammonium salt grafted on the oligomeric chitosan modified with piperazinone diacid is D.

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Abstract

The invention discloses modified oligochitosan for aquaculture and a preparation method thereof. The preparation method includes: adopting piperazinedione acid to graft C2-NH2 on oligochitosan high in antibacterial performance; grafting quaternary ammonium salt with C6-OH on oligochitosan modified by piperazinedione acid to prepare and synthesize the modified oligochitosan. The preparation method is simple to operate and safe in operation environment, and both antibacterial performance and dissolubility of an oligochitosan derivative obtained are improved obviously.

Description

technical field [0001] The invention belongs to the field of aquaculture, and more particularly, the invention relates to a modified chitosan oligomer for aquaculture and a preparation method thereof. Background technique [0002] Chitosan is the product of deacetylation of chitin, an alkaline polysaccharide, and one of the few charged natural products. It has a wide range of sources, is non-toxic, harmless, no residue, no drug resistance, safe and efficient, and is a feed additive with great development value. [0003] Chitosan has obvious inhibitory and killing effects on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans and other bacteria, showing strong antibacterial activity. However, compared with the existing chemically synthesized antibacterial agents, the antibacterial activity is still low and there is basically no inhibitory activity against fungi. In addition, the inhibitory effect of chitosan is also weakened due to the increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A01P1/00A01P3/00
CPCC08B37/003
Inventor 郭瑞忠
Owner 宁波科瑞特动物药业有限公司
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