Method for cultivating hepatitis E viruses

A technology of hepatitis E virus and virus, which is applied in the field of successfully culturing hepatitis E virus strains in vitro and improving the replication efficiency of progeny viruses, can solve the problems of restricted replication of HEV virus, and achieve the effect of promoting replication
CN105671004AInactive Publication Date: 2016-06-15KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2016-06-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for cultivating hepatitis E viruses in an in-vitro manner. The method has the advantages that miRNA-A6 [micro-RNA (ribonucleic acid)-A6] is transfected by cells for 24 h, then viruses are inoculated and can be massively replicated, and mass replication of offspring viruses of the HEV (hepatitis E viruses) can be promoted by the transfected miRNA-A6 as revealed by means of Real-Time qPCR (quantitative polymerase chain reaction) and Western Blot analysis; the method is major breakthrough in the aspect of HEV in-vitro cultivation, and excellent foundation is laid for further researching biological characteristics and immunological characteristics of the HEV, researching and developing HEV vaccine and screening anti-HEV medicines.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a new method for successfully cultivating hepatitis E virus strains in vitro and improving the replication efficiency of progeny viruses. Background technique

[0002] Hepatitis E virus (HEV) is a viral hepatitis pathogen that spreads through the intestinal tract and seriously endangers human health, and can infect humans and various animals across species. HEV is a non-encapsulated single-stranded positive-sense RNA virus, spherical in shape, 27-34nm in diameter and non-encapsulated. The nucleocapsid is icosahedral in three-dimensional symmetry, with protrusions and nicks on the surface, and uneven internal density. The total length of the genome is about 7.3 kb, consisting of 3 open reading frames. There are mainly four genotypes of HEV in the world, and type IV HEV strains are common in China. The transmission route of HEV is mainly through the fecal-oral route, but can also be tr...

Claims

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