Preparation method of 16 beta-methyl steroid
A technology of methyl steroids and compounds, which is applied in the field of preparation of 16-position β-methyl steroids, can solve the problems of complex steps, many side reactions, and low yields, and achieve simple steps, convenient operation, and high yields Effect
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Embodiment 1
[0037] A kind of preparation method of 16β-methyl steroid compound of the present invention, take 16α-methyl steroid compound Ia1 as starting material, carry out enolization reaction under the catalysis of base, prepare 16β-methyl steroid compound Ⅱa1, the reaction scheme is as follows:
[0038]
[0039] The preparation method specifically comprises the following steps:
[0040] In a three-necked flask with a thermometer and a Teflon mechanical stirring rod, add 100g of compound Ia1, 600ml of tetrahydrofuran (THF), replace with nitrogen three times, stir well, add 40g of potassium tert-butoxide, and stir at room temperature (20-25°C) React for 1 hour, then slowly lower the temperature to 0°C, keep stirring for 30 minutes, then continue to slowly cool down to -60°C and stir for 2 hours. When TLC or HPLC detects that the main raw material is less than 3%, stop the reaction, slowly drop 50ml of water to quench the reaction, and then dropwise add glacial acetic acid to adjust ...
Embodiment 2
[0043] A kind of preparation method of 16β-methyl steroid compound of the present invention, take compound Ia2 as starting material, carry out enolization reaction under the catalysis of acid, prepare 16β-methyl steroid compound IIa2, the reaction route is as follows:
[0044]
[0045] The preparation method specifically comprises the following steps:
[0046] In a three-necked flask with a thermometer and a Teflon mechanical stirring rod, add 100g of compound Ia2, 300ml of acetone, replace with nitrogen three times, stir evenly, add 10g of concentrated hydrochloric acid (mass fraction is 30%), heat up to 65 ° C, reflux and stir 1h, slowly cool down to 25°C, the cooling rate is 5°C / h, keep stirring at 25°C for 6h, continue to cool down to 0°C and stir for 3h. Add triethylamine to adjust the system to neutral, suction filter, wash the filter cake with a small amount of acetone, then wash with 200ml of water, and dry at 60°C to obtain 98g of crude product with HPLC purity of 95...
Embodiment 3
[0049] A kind of preparation method of 16β-methyl steroid compound of the present invention, take compound Ib1 as starting material, react under the catalysis of base, prepare 16β-methyl steroid compound IIa3, the reaction scheme is as follows:
[0050]
[0051] The preparation method specifically comprises the following steps:
[0052] In a three-necked flask with a thermometer and a Teflon mechanical stirring rod, add 100g of compound Ib1, 600mlTHF, replace with nitrogen three times, stir evenly, add 40g of potassium methoxide, stir and react at room temperature (20-25°C) for 1h, then slowly cool down to 0°C, keep stirring for 30min, then continue to slowly cool down to -60°C and stir for 2h. TLC detected that the main raw material of the reaction was less than 3%, stopped the reaction, slowly added 50ml of water dropwise to quench the reaction, then added dropwise 20% hydrochloric acid to adjust the system to acidity, pH = 2-3, and stirred for 30min. The completion of h...
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