Preparation method of compound microorganism demulsifying agent
A technology of compound microorganism and demulsifier, which is applied in the field of preparation of compound microorganism demulsifier, can solve the problems of low dehydration rate, slow dehydration speed and poor effect, and achieve the effect of simple preparation steps, fast dehydration speed and good effect
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example 1
[0016] First, in parts by weight, select 15 parts of agar, 5 parts of glycerin, 5 parts of glucose, 10 parts of peptone and 65 parts of water, stir and mix them at 160r / min, and place them in a shaker for 50 minutes to obtain a culture solution. Then, dig 1kg of bottom mud from the place 10m below the surface of the oilfield and put it into a petri dish, add the culture solution to the bottom mud according to the solid-liquid ratio of bottom mud and culture solution 2:1, control the temperature at 20°C, and cultivate for 3 day, the culture medium was changed every 10 hours to obtain a mixture; the mixture obtained above was put into a fermenter, and 3% betaine of the mixture mass was added thereto at the same time, the fermentation temperature was controlled to be 28°C, and the fermentation was sealed for 3 days, and then the Place it in a centrifuge at 3000r / min for 10 minutes to obtain a fermented liquid; weigh 0.5kg of soybean stalks, wash them twice with clean water, and pl...
example 2
[0019] First, in parts by weight, select 23 parts of agar, 7 parts of glycerin, 8 parts of glucose, 12 parts of peptone and 50 parts of water, stir and mix at 160 r / min, and place in a shaker for 55 minutes to obtain a culture solution Then, dig 2kg of bottom mud from the place 15m below the surface of the oilfield and put it into a culture dish, add the culture solution to the bottom mud according to the solid-liquid ratio of bottom mud and culture solution at 2:1, control the temperature at 22°C, and cultivate for 4 day, the culture medium was changed every 13 hours to obtain a mixture; the mixture obtained above was put into a fermenter, and at the same time, betaine with 4% of the mixture mass was added thereto, the fermentation temperature was controlled to be 32°C, and the fermentation was sealed for 4 days, and then the Place it in a 3000r / min centrifuge for 13 minutes to obtain a fermentation broth; weigh 0.6kg of soybean stalks, wash them with clean water for 3 times, ...
example 3
[0022]First, in parts by weight, select 30 parts of agar, 10 parts of glycerin, 10 parts of glucose, 15 parts of peptone and 35 parts of water, stir and mix at 160 r / min, and place in a shaker for 60 minutes to obtain a culture solution ; then dig 2kg of bottom mud from the oilfield surface 20m and put it into a culture dish, add the culture solution to the bottom mud according to the solid-liquid ratio of bottom mud and culture solution 2:1, control the temperature at 24°C, and cultivate for 5 day, the culture medium was changed every 16 hours to obtain a mixture; the mixture obtained above was put into a fermenter, and 5% betaine of the mixture mass was added thereto at the same time, the fermentation temperature was controlled to be 35°C, and the fermentation was sealed for 5 days, and then the Place it in a centrifuge at 3000r / min for 15 minutes to obtain a fermented liquid; weigh 0.7kg of soybean stalks, rinse them with clean water for 3 times, and place them in a ventilat...
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