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Zein and glycopolypeptide grafted copolymer and preparation method and application thereof

A zein and graft technology, which is applied in the field of chemical modification of natural protein and its medical application, can solve problems such as no research work, and achieves favorable cell growth, good biocompatibility and degradability. , the effect of low price

Active Publication Date: 2016-06-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, glycopolypeptide biomimetic polymers have been used to prepare bioactive polymer vesicles and polymer micelles, but there is no research work on using it to modify zein to construct tissue engineering specific cell scaffold material

Method used

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  • Zein and glycopolypeptide grafted copolymer and preparation method and application thereof
  • Zein and glycopolypeptide grafted copolymer and preparation method and application thereof
  • Zein and glycopolypeptide grafted copolymer and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of Zein and Glycopolypeptide Graft (ZG-1) and Electrospun Membrane

[0045] (1) Preparation of galactose pentaacetate

[0046] D-galactose (5.00 g) was dissolved in 15 mL of pyridine, and acetic anhydride (27 mL) was added at 0°C under nitrogen protection. After reacting for 24 hours, it was slowly poured into ice water (100 mL), and extracted three times with ethyl acetate. Use saturated NaHCO for the organic phase 3 The solution is cleaned until no gas is generated, and then washed with water and brine for 3 times. The organic phase was dried with anhydrous magnesium sulfate and then rotary evaporated to obtain an oily product with a yield of 97%.

[0047] (2) Preparation of 3-ynbutyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside

[0048] Dissolve galactose pentaacetate (10.00 g) and 3-butyn-1-ol (2.3 mL) in 50 mL of dry dichloromethane, add Et dropwise at 0°C under nitrogen protection 2 O·BF 3 (4.8mL), react at room temperature for 2h. Dilute with dic...

Embodiment 2

[0066] Example 2 Preparation of Zein and Glycopolypeptide Graft (ZG-2) and its Electrospun Membrane

[0067] (1) Preparation of galactose pentaacetate

[0068] D-galactose (5.00 g) was dissolved in 15 mL of pyridine, and acetic anhydride (27 mL) was added at 0°C under nitrogen protection. After reacting for 24 hours, it was slowly poured into ice water (100 mL), and extracted three times with ethyl acetate. Use saturated NaHCO for the organic phase 3 The solution is cleaned until no gas is generated, and then washed with water and brine for 3 times. The organic phase was dried with anhydrous magnesium sulfate and then rotary evaporated to obtain an oily product with a yield of 97%.

[0069] (2) Preparation of 3-ynbutyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside

[0070] Dissolve galactose pentaacetate (10.00 g) and 3-butyn-1-ol (2.3 mL) in 50 mL of dry dichloromethane, add Et dropwise at 0°C under nitrogen protection 2 O·BF 3 (4.8mL), react at room temperature for 2h. Dilute with...

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Abstract

The invention discloses a zein and glycopolypeptide grafted copolymer and a preparation method and application thereof. The preparation method comprises the steps that firstly, inherent amidogens of zein macromolecules are utilized to trigger an N-carboxyanhydride monomer containing azide functional groups for ring opening polymerization, azide-containing side-group polypeptide modified zein is obtained, and galactose is grafted to a polypeptide chain segment side group so as to obtain the zein and glycopolypeptide grafted copolymer. The zein and glycopolypeptide grafted copolymer prepared by means of the preparation method can be used for preparing a corresponding electrospun fibrous membrane serving as a liver cell scaffold material by adopting an electrospinning technique. The main raw material used for the zein and glycopolypeptide grafted copolymer belongs to a natural vegetable protein, a grafted glycopolypeptide chain segment is of a chemical structure similar to a glycoprotein in a living body, and therefore the prepared zein and glycopolypeptide grafted copolymer and the electrospun membrane scaffold material thereof have very good biocompatibility and bionic characteristics.

Description

Technical field [0001] The invention belongs to the field of natural protein chemical modification and its medical application, and specifically relates to a graft of zein and glycopolypeptide, a preparation method thereof and its application in the construction of hepatocyte scaffold materials. Background technique [0002] Zein, derived from major crops, is a kind of natural polymer with a large quantity and wide range, non-toxic and easily biodegradable. In recent years, it has been used as a biomedical material, especially for the modification and high-value utilization of tissue engineering scaffold materials. , Is gradually attracting great attention from domestic and foreign scholars. Compared with the synthetic polymer materials commonly used in the construction of tissue engineering scaffolds, such as polylactic acid, polycaprolactone, polycarbonate, polyglycolic acid, etc., this kind of natural polymer not only has abundant raw materials, is renewable and has good bioco...

Claims

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Application Information

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IPC IPC(8): C08G81/00C12N5/071
CPCC12N5/067C08G81/00C12N2533/50
Inventor 张黎明缪迎羚
Owner SUN YAT SEN UNIV
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