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Method for extracting sea buckthorn fruit oil in grading mode through combination of aqueous enzymatic method and ultrasonic assistance

A seabuckthorn fruit oil, ultrasonic-assisted technology, applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc., can solve the problems of low oil yield, poisonous, harmful to the human environment, etc., to achieve the preservation of nutritional quality, process The effect of low cost and easy operation

Inactive Publication Date: 2016-06-29
TRUSYN CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem mainly solved by the present invention: in the extraction method of seabuckthorn fruit oil commonly used at present, the natural sedimentation method and the three separation methods have a relatively low oil yield, while the solvent extraction method has a high oil yield, but there is still an organic solvent part Residues in the oil are toxic, odorous, and harmful to the human body and the environment, and the recovery of organic solvents has potential safety hazards and the current situation of insufficient utilization of seabuckthorn fruit resources. A method for squeezing seabuckthorn fruit and separating juice and filter residues is provided. First use enzymatic hydrolysis and ultrasound to assist in extracting the fruit oil in the fruit juice, then freeze the pomace for alcohol precipitation to further obtain the fruit oil in the pomace, combine the two fruit oils to improve the precision of the fruit oil by supercritical carbon dioxide method, so as to obtain the water enzyme A method for extracting seabuckthorn fruit oil by graded extraction method and ultrasound-assisted combination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] First, wash 2kg of fresh seabuckthorn fruit and put it into a juice extractor to extract the juice, filter and separate to obtain the juice, and use the pomace for later use. 1% pectinase, stir evenly with a stirring rod, seal the mouth of the tank, put it in an incubator and raise the temperature to 40°C, and keep it warm for 10 hours for enzymolysis; then transfer the enzymolysis solution into an ultrasonic extractor after enzymolysis, and use 100W Ultrasonic extraction with power for 30 minutes, then poured into a horizontal centrifuge, set the speed at 7000r / min, centrifuged at a high speed for 10 minutes at room temperature, separated the upper oil layer, and set aside; after that, add the above-mentioned standby seabuckthorn fruit filter residue to deionized In water, heat the water bath to 90°C and keep boiling for 10 minutes to break the cell wall and precipitate the fruit oil, then add anhydrous ethanol twice its volume to the mixed solution after the fruit oil ...

example 2

[0027]First, wash 2.5kg of fresh seabuckthorn fruit and put it into a juicer to extract the juice, filter and separate to obtain the juice, leave the pomace for later use, pour 550mL of the squeezed juice into the enzymatic hydrolysis tank, and add 0.8% of the total amount of juice to break the wall enzyme and 1.5% pectinase, stir evenly with a stirring rod, seal the mouth of the tank, put it in an incubator and raise the temperature to 45°C, and keep it warm for 11 hours for enzymolysis; Ultrasonic extraction for 35 minutes, then poured into a horizontal centrifuge, set the speed at 7500r / min, centrifuged at a high speed for 13 minutes at room temperature, separated the upper oil layer, and set it aside; after that, add the above-mentioned standby seabuckthorn fruit filter residue to the 4 times its mass. In ionized water, heat the water bath to 95°C and keep boiling for 13 minutes to break the cell wall and precipitate the fruit oil, then add 2.5 times the volume of absolute ...

example 3

[0030] First, wash 3kg of fresh seabuckthorn fruit and put it into a juice extractor to squeeze the juice, filter and separate to obtain the juice, and use the pomace for later use. Pour 600mL of the squeezed juice into the enzymatic hydrolysis tank, add 1% of the total amount of the juice to break the wall enzyme and 2% pectinase, stir evenly with a stirring rod, seal the mouth of the tank, put it in an incubator and raise the temperature to 50°C, keep it warm for 12 hours; Power ultrasonic extraction for 40 minutes, then poured into a horizontal centrifuge, set the speed at 8000r / min, centrifuged at a high speed for 15 minutes at room temperature, separated to obtain the upper oil layer, and set aside; after that, add the above-mentioned standby seabuckthorn fruit filter residue to deionized 5 times its mass In water, heat the water bath to 100°C and keep boiling for 15 minutes to break the cell wall and precipitate the fruit oil, then add anhydrous ethanol three times its vo...

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Abstract

The invention discloses a method for extracting sea-buckthorn fruit oil in combination with an aqueous enzymatic method and ultrasonic assistance, and belongs to the field of extracting sea-buckthorn fruit oil. In the present invention, the seabuckthorn fruit is squeezed, the juice and the filter residue are separated, the fruit oil in the fruit juice is firstly extracted with the aid of enzymolysis and ultrasound, and then the fruit residue is frozen and alcohol-precipitated to further obtain the fruit oil in the fruit residue, and the fruit oil is combined twice through The supercritical carbon dioxide method improves the precision of the fruit oil, thereby obtaining a method for extracting seabuckthorn fruit oil in stages using the hydroenzyme method and ultrasonic assistance. Examples prove that the present invention is safe and easy to operate, and the hydroenzyme method and ultrasonic-assisted method are combined to extract seabuckthorn fruits by grade, which not only ensures the oil yield of seabuckthorn fruits, but also retains its nutritional quality, so that the resources of seabuckthorn fruits are fully obtained. The seabuckthorn fruit oil is perfect in color, non-toxic, tasteless, without any organic solvent residue, and has no harm to the human body and the environment.

Description

technical field [0001] The invention discloses a method for extracting sea-buckthorn fruit oil in combination with an aqueous enzymatic method and ultrasonic assistance, and belongs to the field of extracting sea-buckthorn fruit oil. Background technique [0002] Seabuckthorn is a rare herbal medicine that can grow under harsh conditions and contains a variety of active ingredients. It is a common Chinese medicine used by Tibetan and Mongolian doctors in ancient my country to treat diseases. Pharmacological efficacy of promoting blood circulation and dispelling diseases. [0003] Seabuckthorn fruit oil is a substance extracted from seabuckthorn fruit by certain physical means. It is a natural substance with high nutrition and high economic value. It is composed of palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid Acid, linolenic acid and other beneficial fatty acids, of which unsaturated fatty acids are as high as 70%, carotenoids and vitamin E in seab...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/04C11B1/10C11B1/14C11B3/00C11B3/12
CPCC11B1/025C11B1/04C11B1/106C11B1/14C11B3/001C11B3/006C11B3/12
Inventor 孙春辉张帆林大伟
Owner TRUSYN CHEM TECH
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