Method for producing organic fertilizer without pile turning
A production method and technology of organic fertilizer, which are applied in the preparation of organic fertilizer, organic fertilizer, and biological organic part treatment, etc., can solve the problems of inconvenient transportation, centralized treatment, inconvenient production of organic fertilizer, waste of solid waste, etc., and save power consumption. and labor costs, saving labor and energy costs, and saving inputs
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Embodiment 1
[0026] Example 1: Insert the thermostable facultative anaerobic bacteria Bacillus methylotrophicus HL-4 preserved on the slant into the LB liquid medium cooled after sterilization, cultivate it at 55°C and 150 r / min for 12 hours, and prepare Bacillus methylotrophicus HL-4 seed liquid for later use ; Mix bran and water in a mass ratio of 1:1, and mix well to prepare bran medium. Bran culture is based on sterilization at 121°C for 30 minutes before use; the prepared Bacillusmethylotrophicus HL-4 seed solution is inserted into After sterilization, the cooled bran culture medium is mixed evenly, and then the bran culture medium in which the strains are introduced is divided into sterilized fermentation pans, the thickness of the filling is 4cm, and the surface of the bran culture medium in the shallow pan is Cover with sterilized wet gauze, then place the tray on the shelf in the sterilized solid fermentation room to ferment, control the ambient temperature at 55°C, and ferment for...
Embodiment 2
[0027] Example 2: Insert the thermostable facultative anaerobic bacteria Bacillus methylotrophicus HL-4 preserved on the slant into the LB liquid medium cooled after sterilization, and cultivate it at 55°C and 150 r / min for 12 hours, and prepare Bacillus methylotrophicus HL-4 seed liquid for later use ; Mix bran and water in a mass ratio of 1:1, and mix well to prepare bran medium. Bran culture is based on sterilization at 121°C for 30 minutes before use; the prepared Bacillusmethylotrophicus HL-4 seed solution is inserted into After sterilization, put the bran medium in the cooled bran medium and mix it evenly, then divide the bran medium into which the strains are inserted into the sterilized fermentation shallow pan, the thickness of the filling is 4cm, and the surface of the fermentation medium in the shallow pan is covered Sterilized wet gauze, and then put the tray on the shelf in the sterilized solid fermentation room to ferment, control the ambient temperature at 55°C, ...
Embodiment 3
[0028]Example 3: Insert the thermostable facultative anaerobic bacteria Bacillus methylotrophicus HL-4 preserved on the slant into the LB liquid medium cooled after sterilization, and cultivate it at 55°C and 150 r / min for 12 hours, and prepare Bacillus methylotrophicus HL-4 seed liquid for later use ; Mix bran and water in a mass ratio of 1:1, and mix well to prepare bran medium. Bran culture is based on sterilization at 121°C for 30 minutes before use; the prepared Bacillusmethylotrophicus HL-4 seed solution is inserted into After sterilization, put the bran medium in the cooled bran medium and mix it evenly, then divide the bran medium into which the strains are inserted into the sterilized fermentation shallow pan, the thickness of the filling is 4cm, and the surface of the fermentation medium in the shallow pan is covered Sterilized wet gauze, and then put the tray on the shelf in the sterilized solid fermentation room to ferment, control the ambient temperature at 55°C, a...
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