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Extraction method of keratan sulfate in egg white

A technology of keratan sulfate and an extraction method, applied in the field of keratan sulfate extraction, can solve problems such as limited output and lack of keratan sulfate products, and achieve the effects of stable molecular weight consistency, expanded sources, and wide sources.

Active Publication Date: 2017-12-22
刘长国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, keratan sulfate mainly comes from bull's eye cornea, and the output is limited. In addition to the outbreak of mad cow disease, there is a shortage of keratan sulfate products on the market.

Method used

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Embodiment Construction

[0027] The present invention will be described in further detail below in conjunction with embodiment. It should be understood that the specific embodiments described here are only used to explain the present invention, but not to limit the present invention.

[0028] Preparation and handling of egg whites:

[0029] (1) Liquid separation of egg whites and egg yolks: After opening the eggs, use an egg white and yolk separator to separate the egg whites and egg yolks and collect the egg whites. After the egg white is separated, there are three treatment options: A. If the follow-up processing is carried out within 48 hours, keep it fresh and refrigerated at a low temperature of 2-10°C; B. If the egg white is to be stored within 3 months, store it at -20-- Freeze at 30°C. If egg whites are to be stored for 3 months to 1 year, store them at -70°C. Quick-frozen in liquid nitrogen, then freeze-dried (no crushing required).

[0030] (2) Solid-state separation of egg whites and egg...

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Abstract

The invention discloses an extraction method for keratin sulfate in egg white.The extraction method comprises the steps that the egg white is acquired; total glycosaminoglycan is separated and extracted from the egg white; heparin or heparan sulfate, chondroitin sulfate or dermatan sulfate and hyaluronic acid in the total glycosaminoglycan are removed, and then the high-purity keratin sulfate is obtained.

Description

technical field [0001] The invention relates to a method for extracting keratan sulfate, in particular to a method for extracting keratan sulfate from egg white. Background technique [0002] Keratan sulfate is a class of glycosaminoglycans, that is, straight-chain linear macromolecular polysaccharides rich in anions. Like other glycosaminoglycans, keratan sulfate in animals can combine with various cell growth factors, cytokines, cell receptors and biochemical enzymes to induce signal transduction, regulate cell growth, differentiation and apoptosis; Water content in tissues and organs. At present, keratan sulfate is mainly developed into a drug for the treatment of ophthalmic diseases, such as eye inflammation, eye uveitis, keratitis, and corneal injury; it is also used as the main component of eye drops for eye care. [0003] At present, keratan sulfate mainly comes from bull's eye cornea, and the output is limited. In addition to the outbreak of mad cow disease, there ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0063
Inventor 刘长国赵红娟
Owner 刘长国
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