Selective Enterococcus Enrichment Medium

Abstract

The invention provides a selective enterococcus enrichment medium. According to the technical scheme, the medium comprises the following components: 5.0 g of proteose peptone, 2.5 g of yeast extract powder, 3.0 g of beef extract, 20.0 g of glucose, 4.0 g of lactose, 5.0 g of sodium chloride, 2.5 g of sodium azide, 10.0 g of bile salt, 6 ml of an aqueous solution with a bromocresol green concentration being 0.1% and an ethanol concentration being 20%, and 1000 ml of distilled water. The medium is manufactured by weighing 5.0 g of proteose peptone, 2.5 g of yeast extract powder, 3.0 g of beef extract, 20.0 g of glucose, 4.0 g of lactose, 5.0 g of sodium chloride, 2.5 g of sodium azide, and 10.0 g of bile salt; putting the obtained materials in a 1000 ml conical flask; measuring 1000 ml of distilled water and putting the distilled water in the conical flask; heating the conical flask to melt the obtained mixture; adjusting the pH value to 6.0; adding 6 ml of the aqueous solution with a bromocresol green concentration being 0.1% and an ethanol concentration being 20%; split-charging 50.0 ml of the obtained solution in a 150 ml conical flask and binding the 150 ml conical flask up; and sterilizing the bound 150 ml conical flask for 15 min at a temperature of 121 DEG C for later use. Compared with an AC broth medium, the selective enterococcus enrichment medium is more obvious in color change and easier in result observation.

Description

technical field [0001] The invention relates to the technical field of microbial culture medium, in particular to a selective enterococcus enrichment culture medium. Background technique [0002] Recent studies have shown that most enterococci can produce bacteriocins, which are considered as opportunistic pathogens and can cause: urinary tract infection, abdominal cavity, pelvic cavity infection, wound infection after burns, etc. It is an important hospital infection second only to Staphylococcus pathogenic bacteria. Food poisoning caused by Enterococcus has been reported in my country in recent years, most of which are caused by Enterococcus faecalis and its variants, Enterococcus faecium, and Enterococcus gallinarum; food poisoning caused by Enterococcus saccharolyticum and Enterococcus avoid have been reported abroad. If the amount of enterococcus from clinical specimens is small or the enterococcus of food samples is damaged during the production process, it is not eas...

AI Technical Summary

Problems solved by technology

At present, the effect of the existing AC broth medium and enterococcus broth (all positive medium for hydrolyzed aescin) is not very satisfactory ( figure 1 , figure 2 )

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