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Cultivation medium for liquid strains of flammulina velutipes and method for preparing cultivation medium

A liquid strain and culture medium technology, applied in the field of bioengineering, can solve the problems of inability to carry out factory and standardized production, insufficient dissolution of raw materials, slow growth rate of mycelium, etc. Social benefits and low cost effects

Inactive Publication Date: 2016-07-13
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing culture medium of Flammulina velutipes is mainly prepared from corn flour, bean cake powder, potatoes, peptone, white sugar and inorganic salts, and there are many raw materials and high prices and costs.
In the preparation process, long-time cooking is required, and there are problems such as insufficient dissolution of raw materials, many particulate matter, long sterilization time, and complicated preparation process.
The prepared liquid strains have disadvantages such as slow growth of mycelium and long cultivation period, making it impossible to carry out industrialized and standardized production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of Flammulina velutipes liquid culture medium, carried out according to the following steps:

[0029] First weigh the components with the following mass percentage concentrations: 2% corn distiller's grain waste liquid, 1% sucrose, 0.05% potassium dihydrogen phosphate and 0.025% magnesium sulfate, and then add the corn distiller's grain waste liquid into a 70-80°C Stir in 15L hot water and make it dissolve, boil to remove the grease contained therein to obtain the first aqueous solution,

[0030] adding sucrose, potassium dihydrogen phosphate and magnesium sulfate into 15L hot water at 70-80°C, stirring and dissolving to obtain a second aqueous solution,

[0031] uniformly mixing the first aqueous solution and the second aqueous solution to obtain a mixed solution,

[0032] Adjust the pH value of the mixed solution to 6.0 with sodium hydroxide,

[0033] Put the mixed solution after adjusting the pH value into a 50L fermenter for sterilization. The...

Embodiment 2

[0036] A preparation method of Flammulina velutipes liquid culture medium, carried out according to the following steps:

[0037] First weigh the components with the following mass percentage concentrations: 2.5% corn distiller's grain waste liquid, 1.5% sucrose, 0.1% potassium dihydrogen phosphate and 0.05% magnesium sulfate, and then add the corn distiller's grain waste liquid into a 70-80°C Stir in 15L hot water and make it dissolve, boil to remove the grease contained therein to obtain the first aqueous solution,

[0038] adding sucrose, potassium dihydrogen phosphate and magnesium sulfate into 15L hot water at 70-80°C, stirring and dissolving to obtain a second aqueous solution,

[0039] uniformly mixing the first aqueous solution and the second aqueous solution to obtain a mixed solution,

[0040] Adjust the pH value of the mixed solution to 6.0 with sodium hydroxide,

[0041] Put the mixed solution after adjusting the pH value into a 50L fermenter for sterilization. T...

Embodiment 3

[0044] A preparation method of Flammulina velutipes liquid culture medium, carried out according to the following steps:

[0045] First weigh the components with the following mass percentage concentrations: 3% corn distiller's grain waste liquid, 2% sucrose, 0.15% potassium dihydrogen phosphate and 0.075% magnesium sulfate, and then add the corn distiller's grain waste liquid into a 70-80°C Stir in 15L hot water and make it dissolve, boil to remove the grease contained therein to obtain the first aqueous solution,

[0046] adding sucrose, potassium dihydrogen phosphate and magnesium sulfate into 15L hot water at 70-80°C, stirring and dissolving to obtain a second aqueous solution,

[0047] uniformly mixing the first aqueous solution and the second aqueous solution to obtain a mixed solution,

[0048] Adjust the pH value of the mixed solution to 6.0 with sodium hydroxide,

[0049] Put the mixed solution after adjusting the pH value into a 50L fermenter for sterilization. The...

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PUM

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Abstract

The invention discloses a cultivation medium for liquid strains of flammulina velutipes and a method for preparing the cultivation medium.The cultivate medium and the method have the advantages that the cultivation medium contains few components and is easy to obtain and low in cost, waste liquid of distillers' grains generated in procedures for producing alcohol by the aid of fermentation processes is utilized, to be more specific, the waste liquid of the corn distillers' grains is a main composition portion of the cultivation medium and contains 92-95% of water, remaining 5-8% of dry substances in the waste liquid contain abundant nutrition ingredients such as carbohydrate, proteins, celluloses, pentosan, caramel, fat, abundant vitamins B and auxin, and accordingly nutrition ingredients necessary for growth of the liquid strains of the flammulina velutipes can be provided by the cultivation medium; the method for preparing the cultivation medium is relatively simple and includes controllable, simple and convenient operation steps, the problem of pollution due to the waste liquid discharged by an alcohol plant can be solved if the cultivation medium is used for cultivating the liquid strains of the flammulina velutipes, and the cost can be reduced to a great extent; mycelia of the flammulina velutipes can grow fast and are high in activity and content, a formula for the cultivation medium for the liquid strains is favorable for standard production, and the cultivation medium and the method have obvious economic and social benefits.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a liquid culture medium of Flammulina velutipes and a preparation method thereof. Background technique [0002] Flammulina velutipes belongs to Basidiomycetes, Agaricales, Stomataceae, and Flameless. Gain the name Flammulina velutipes because of the golden color of its mushroom body and the slender and elongated stalks like golden needles. Flammulina velutipes is smooth, crisp and tender, delicious, and contains a variety of nutrients. According to the determination, the amino acid content of Flammulina velutipes is very rich, higher than that of common mushrooms, especially the content of lysine is particularly high, and lysine has the function of promoting children's intellectual development. Flammulina velutipes dry product contains 8.87% protein, 60.2% carbohydrate, and 7.4% crude fiber. Regular consumption can prevent and treat ulcer disease. Also have research to show, a ki...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 谢鲲鹏佟少明宫正谢明杰
Owner LIAONING NORMAL UNIVERSITY
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