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Pseudomonas aeruginosa of minks and application of pseudomonas aeruginosa

A technology of mink Pseudomonas aeruginosa and Pseudomonas aeruginosa, applied in the direction of bacteria, antibacterial drugs, veterinary vaccines, etc., can solve the problems of weak immunogenicity and low immune cross-protection, and reduce morbidity and mortality , good immunogenicity, strong immunity effect

Inactive Publication Date: 2016-07-13
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to control mink hemorrhagic pneumonia and solve the problems of weak immunogenicity of existing vaccine strains and low immune cross-protection among various serotypes in the research and development of mink Pseudomonas aeruginosa inactivated vaccines,

Method used

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  • Pseudomonas aeruginosa of minks and application of pseudomonas aeruginosa
  • Pseudomonas aeruginosa of minks and application of pseudomonas aeruginosa
  • Pseudomonas aeruginosa of minks and application of pseudomonas aeruginosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1.1 Isolation and purification method of Pseudomonas aeruginosa bacterial strain

[0019] The affected minks were selected from a large mink farm suspected of hemorrhagic pneumonia in Liaoning Province, and the lungs, liver and heart blood of the sick minks were aseptically collected and inoculated on cetyltrimethylammonium bromide agar medium (NAC) , placed in a 37°C incubator for 16h to 18h, picked round, smooth, moist, and flat colonies for Gram staining and microscopic examination, and selected Gram-negative small bacilli for streaking and pure culture.

[0020] Stratify the pure culture strains on fresh blood agar plate and NAC plate respectively, culture at 37°C for 16h~18h, choose to produce green fluorescent pigment on hexadecanetrimethylammonium bromide medium, and produce β on blood agar medium Hemolyzed small colonies were purified and cultured.

[0021] According to the biochemical identification test requirements of Pseudomonas aeruginosa, the two strains ...

Embodiment 2

[0047] Application of mink Pseudomonas aeruginosa type B LN03 strain in preparation of inactivated vaccine.

[0048] The preservation number of the strain is taken as the Pseudomonas aeruginosa type B LN03 strain with the preservation number CCTCCNO: M2016068. After activation, the culture medium is inoculated respectively, the bacterial liquid is collected after cultivation, inactivated by formaldehyde solution, and mixed with propolis adjuvant to prepare inactivated Propolis vaccine.

[0049] The preferred operation steps are as follows:

[0050] 2.1 Strain selection

[0051] The strain used for seedling production was Pseudomonas aeruginosa type B LN03 strain, which was stable in colony morphology, bacterial characteristics, biochemical characteristics, and culture characteristics, and had strong pathogenicity to minks. 1.0mL bacterial liquid (5.0×10 6 CFU / mL) can cause 100% of 21-42 day-old mink disease. The immunogenicity of mink Pseudomonas aeruginosa type B LN03 stra...

Embodiment 3

[0092] Application of mink Pseudomonas aeruginosa type B LN03 strain in the preparation of inactivated vaccine.

[0093] The preservation number of the strain is taken as Pseudomonas aeruginosa type B LN03 strain with the preservation number CCTCCNO: M2016068. After activation, the culture medium is inoculated respectively, the bacterial liquid is collected after cultivation, inactivated by formaldehyde solution, and mixed with water adjuvant to prepare inactivated vaccine.

[0094] The preferred operation steps are as follows:

[0095] 2.1 Strain selection

[0096] The strain used for seedling production was Pseudomonas aeruginosa type B LN03 strain, which was stable in colony morphology, bacterial characteristics, biochemical characteristics, and culture characteristics, and had strong pathogenicity to minks. 1.0mL bacterial liquid (5.0×10 6 CFU / mL) can cause 100% of 21-42 day-old mink disease. The immunogenicity of mink Pseudomonas aeruginosa type B LN03 strain was good,...

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Abstract

The invention provides pseudomonas aeruginosa of minks and application of the pseudomonas aeruginosa.A preservation number of a type-B pseudomonas aeruginosa strain LN03 of the minks is CCTCC NO.M2016068.The type-B pseudomonas aeruginosa strain LN03 of the minks further can be used for preparing inactivated vaccine of the pseudomonas aeruginosa of the minks.The pseudomonas aeruginosa and the application have the advantages that the type-B pseudomonas aeruginosa strain LN03 is high in pathogenicity for the minks and is excellent in immunogenicity, the inactivated vaccine prepared by the aid of the type-B pseudomonas aeruginosa strain LN03 is safe and reliable, accordingly, homologous attacking protection effects can be realized, the minks are high in immunity after being immunized, the morbidity and the mortality of vaccinated mink groups can be obviously reduced, the epidemiology and propagation of hemorrhagic pneumonia of the minks can be effectively prevented, economic loss due to the hemorrhagic pneumonia can be reduced for the fur animal breeding industries, and the pseudomonas aeruginosa has a broad application prospect.

Description

technical field [0001] The invention relates to the field of mink Pseudomonas aeruginosa vaccine in veterinary biological products, in particular to a mink Pseudomonas aeruginosa type B LN03 strain, and also relates to the application of the mink Pseudomonas aeruginosa type B LN03 strain. Background technique [0002] Mink hemorrhagic pneumonia, also known as mink Pseudomonas pneumonia, is an acute infectious disease that mainly occurs during the moulting season of mink from August to November every year. It is characterized by hemorrhagic pneumonia and acute death. Nostrils flowed out of red foamy liquid and other symptoms. Post-mortem examination showed that the entire lung lobe was enlarged with diffuse hemorrhage and sepsis. The disease occurs all over the world and can cause 20%-40% mortality in farms every year. It is an important bacterial infectious disease that endangers the fur animal breeding industry. [0003] The disease pathogen is Pseudomonas aeruginolsa (Pse...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/104A61K39/39A61P31/04C12R1/385
CPCA61K39/104A61K39/39A61K2039/552A61K2039/55505A61K2039/55588C12N1/205C12R2001/385
Inventor 单虎张洪亮盖春云黄娟张传美秦志华杨瑞梅秦晓冰
Owner QINGDAO AGRI UNIV
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