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Photo-electrochemical DNA biosensor based lead ion determination method

A biosensor, photoelectrochemical technology, applied in the fields of analytical chemistry and photoelectrochemical sensing, can solve the problems of low sensitivity, high background signal, interference, etc., and achieve the effects of high sensitivity, broad application prospects and high selectivity

Active Publication Date: 2016-07-13
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned fluorescent method still has some shortcomings, such as high background signal interference and low sensitivity, etc., so it is limited by many factors in the actual environmental detection

Method used

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  • Photo-electrochemical DNA biosensor based lead ion determination method
  • Photo-electrochemical DNA biosensor based lead ion determination method
  • Photo-electrochemical DNA biosensor based lead ion determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of DNA-modified ITO electrode and biosensor thereof

[0029] The preparation method of the DNA-modified ITO electrode prepared in this embodiment comprises the following steps:

[0030] (1) Clean the ITO glass electrode with detergent, acetone, isopropanol, etc. for 5 minutes, and finally clean it with ultrapure water and dry it in an oven. The concentration of 10% SnO 2 The nano-sol was spin-coated on the surface of ITO conductive glass. After natural air-drying, it was calcined in a muffle furnace at 350°C for 3 hours. After natural cooling, it was cut into 0.5cm×3cm glass pieces with a glass knife to obtain SnO-modified glass pieces. 2 The ITO electrode spare;

[0031] (2) Dilute the solid DNA sample with Tris-HClO 4 The buffer solution was dissolved, placed in a water bath at 85° C. for 10 minutes, then naturally cooled to room temperature, and set aside; wherein, the nucleotide sequence of the DNA is shown in SEQ ID NO:1;

[0032] (3) Apply ...

Embodiment 2

[0036] Embodiment 2 Based on the lead ion determination method of photoelectrochemical DNA biosensor

[0037] Different concentrations of Pb 2+ The solution was dropped on the surface of the DNA-modified ITO electrode prepared in Example 1 and incubated for 2 hours. After the electrode was taken out, it was washed three times with ultrapure water and dried with nitrogen gas. Then add 10μM Ru(bpy) 2 (dppz) 2+ The probe solution was dropped on the surface of the above-mentioned DNA electrode and incubated for 1 h. After washing with ultrapure water for three times, dry it with nitrogen gas, connect it to an electrochemical workstation, and perform cyclic voltammetry scanning under the irradiation of 473nm blue light to realize the detection of Pb 2+ determination. Different concentrations of Pb 2+ The photoelectrochemical signals generated after interacting with the ITO electrode DNA-modified membrane are shown in Table 1.

[0038] Table 1 Different concentrations of Pb 2...

Embodiment 3Pb2

[0041] Example 3Pb 2+ selective research

[0042] With 100nM metal ion Pb 2+ , Hg 2+ , Mg 2+ , Ca 2+ , Fe 2+ , Zn 2+ Instead of Pb in Example 2 2+ , other experimental conditions are identical with embodiment 2. Changes of photoelectrochemical signals produced by different metal ions interacting with DNA-modified membranes of ITO electrodes Δ I see Table 2.

[0043] Table 2 Changes in photoelectrochemical signal ΔI produced by different heavy metal ions

[0044]

[0045] It can be seen from Table 2 that when Pb 2+ After interacting with the surface of the DNA-modified ITO electrode, the change in the photoelectrochemical signal is the largest, while the change in the photoelectrochemical signal produced by several other metal ions is small. The results show that the photoelectrochemical DNA biosensor can realize the detection of Pb 2+ selective detection.

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Abstract

The invention provides a photo-electrochemical DNA biosensor based lead ion determination method. According to the method, a DNA sequence containing partial Pb<2+> which can be specifically identified is assembled on the surface of an ITO electrode; and with Ru(bpy)2(dppz)<2+> as a photo-electrochemical signal probe, after the Pb<2+> acts with DNA on the surface of the electrode, the probe is separated from a DNA chain, so that a photo-electrochemical signal is weakened, and photo-electrochemical detection of Pb<2+> is achieved. The DNA biosensor with the ITO electrode provided by the invention is simple in preparation, low in cost, high in response speed, convenient in detection, short in period, high in stability and good in repeatability, and has mild reaction conditions. Furthermore, the photo-electrochemical sensor has the advantages of high selectivity, high sensitivity and the like on Pb<2+>.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and photoelectrochemical sensing, and in particular relates to a method for measuring lead ions based on a photoelectrochemical DNA biosensor. Background technique [0002] Heavy metals are an important class of pollutants in the environmental system. They are difficult to degrade, highly toxic, and bioaccumulative. They pose serious hazards to ecosystems and human health. Therefore, the rapid, simple, and sensitive detection of heavy metal ions is of great significance. . Traditional heavy metal ion analysis methods mainly use atomic fluorescence, atomic emission spectrometry, ICP-AES / MS, etc. These methods have disadvantages such as complex operation, expensive equipment, high detection cost, and difficulty in online analysis. With the development of biosensing technology, the detection of toxic heavy metal ions by biosensing has become one of the research hotspots. Compared with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 梁刚贾文珅满燕靳欣欣潘立刚
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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