System for focusing or separating micro-nano particles and cells on basis of non-Newton effect, and method thereof

A technology of micro-nano particles and non-Newtonian effect, which is applied in the direction of chemical instruments and methods, biochemical equipment and methods, and laboratory containers, etc. It can solve the problems of irreversible damage to samples, restrictions on widespread popularization, and cumbersome operation steps. Achieve the effect of avoiding irreversible damage, easy operation and good focusing effect

Active Publication Date: 2016-07-20
INST OF MECHANICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods generally have the disadvantages of low capture efficiency, cumbersome operation steps, low sensitivity, and easy to cause irreversible damage to the sample.
Mature commercialized flow cytometers require high costs (instrument cost, reagent consumption, skilled operators), which limits their widespread adoption

Method used

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  • System for focusing or separating micro-nano particles and cells on basis of non-Newton effect, and method thereof
  • System for focusing or separating micro-nano particles and cells on basis of non-Newton effect, and method thereof
  • System for focusing or separating micro-nano particles and cells on basis of non-Newton effect, and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment proposes a system and method for focusing micro-nano particles based on the non-Newtonian effect, such as figure 1 As shown, the system includes a working fluid delivery device 1, a microfluidic unit 2 containing a microchannel, and an outlet 3 for collecting focused micro-nano particles.

[0035] In the present invention, the working fluid delivery device 1 is used to deliver the working fluid, which can be a syringe pump, a peristaltic pump or other instruments that drive the working fluid with pressure and precisely control the flow rate, or can use gravity or other physical fields to make Micro-nanoparticles or cells flow into the microchannels with precise flow rates.

[0036] In this embodiment, the working fluid delivery device 1 is a syringe pump, which is used to deliver working fluid containing micro-nano particles to be focused.

[0037] The chip of microfluidic unit 2 can be made of polydimethylsiloxane (PDMS), polymethyl methacrylate (PMMA)...

Embodiment 2

[0057] This example presents systems and methods for focusing red and white blood cells in whole blood, such as image 3 As shown, the system includes a working fluid delivery device 1 , a microfluidic unit 2 and an outlet 3 for collecting red blood cells and white blood cells.

[0058] In the present invention, the working fluid delivery device 1 can transport the working fluid by a syringe pump, a peristaltic pump or other instruments that drive the working fluid with pressure and precisely control the flow rate, and can also use gravity or other physical fields to make the micro-nano Particles or cells flow precisely into the microchannels. The working fluid delivery device 1 drives the working fluid through a syringe pump to deliver the cells to be focused; in this example, it is the flow of blood in the microchannel and adjusts the flow rate of the blood in the microchannel.

[0059] The chip of the microfluidic unit 2 can be made of PDMS, PMMA, PC, and the bottom sealin...

Embodiment 3

[0080] This example presents a system for separating micro-nanoparticles, such as Figure 7 As shown, the system includes a working fluid delivery device 1 , a microfluidic unit 2 and a bifurcated outlet 3 for collecting particles of different sizes.

[0081] In the present invention, the working fluid delivery device 1 can transport the working fluid by a syringe pump, a peristaltic pump or other instruments that drive the working fluid with pressure and precisely control the flow rate, and can also use gravity or other physical fields to make the micro-nano The particles flow precisely into the microchannels. The working fluid delivery device 1 drives the working fluid through a syringe pump for transporting micro-nanoparticles; in this example, the working fluid containing the micro-nanoparticles to be separated flows in the microchannel and adjusts the flow of the working fluid in the microchannel flow rate.

[0082] The chip of the microfluidic unit 2 can be made of PDM...

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Abstract

The invention discloses a system for focusing or separating micro-nano particles and cells on basis of a non-Newton effect. The system is characterized in that the system comprises: a working fluid having a non-Newton fluid effect; a working fluid conveying device used for conveying the working fluid having a non-Newton fluid effect; micro-fluidic units including micro-channels, wherein the micro-fluidic units are arranged on the working fluid conveying device in parallel, are connected with the working fluid conveying device through a pipeline and are used for focusing or separating the micro-nano particles or cells conveyed by the working fluid conveying device; and an outlet arranged at the tail ends of the micro-fluidic units and used for collecting target micro-nano particles or cells, wherein no branch outlets are arranged when the outlet is used for focusing; and the quantity and the width of the branch outlets are set according to separation characteristics when the outlet is used for separating in order to make the micro-nano particles or cells with different dimension to flow into different branch outlets. The system and the method realize simple operation and good effect of separation and focusing of the micro-nano particles and the cells.

Description

technical field [0001] The invention relates to a system and method for focusing and separating micro-nano particles and cells based on the non-Newton effect. Background technique [0002] Continuously manipulating (such as focusing and separating) micro-nanoparticles and cells is of great significance in many applications and research fields such as medical treatment, pharmaceuticals, hematology, and microbiology. Flow cytometry is widely used in these fields, in which micro-nanoparticles and cells need to be focused into a single queue in space before they can be effectively detected and analyzed by fluorescence activation and laser scattering methods. The focusing position should be consistent with the average distance between particles and cells and the depth direction. [0003] The current mainstream method for spatially focusing micro-nanoparticles and cells is to use sheath flow. The sheath fluid on both sides flows from the sheath fluid tube to the nozzle hole, surr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/00
Inventor 胡国庆刘超孙佳姝
Owner INST OF MECHANICS - CHINESE ACAD OF SCI
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