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Method for extracting hypericin from Hypericum perforatum

A technology of Hypericum perforatum and hypericin, applied in organic chemistry, quinone separation/purification, etc., can solve the problem of low extraction rate, achieve the effect of increasing extraction rate, shortening solvent extraction time, and increasing content

Inactive Publication Date: 2016-07-20
陕西笃诚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] CN1436562A and CN1689596A respectively disclose the use of column chromatography to separate and purify hypericin. Although the method has obtained an extract with a content of hypericin of 0.6%, its yield is only 2.7%. It can be seen from conversion that hypericin The extraction rate is only 0.0162%
[0010] CN1850766A After obtaining hypericin concentrate, utilize macroporous adsorption resin to separate and purify hypericin, the content of hypericin in the obtained extract is 0.9%, its yield is only 2-5 / 10,000, and the extraction rate is lower

Method used

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  • Method for extracting hypericin from Hypericum perforatum
  • Method for extracting hypericin from Hypericum perforatum
  • Method for extracting hypericin from Hypericum perforatum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the kind of biological enzyme and dosage screening

[0046] Step (1): Processing raw materials

[0047] After washing the flowers and leaves of Hypericum perforatum, put them in a blast drying oven, dry them at a temperature of 35°C until the water content is less than 10%, then crush them with high speed shearing, sieve, and collect the parts with a particle size of 100-200 mesh , to obtain Hypericum perforatum powder.

[0048] Step (2): Enzyme treatment

[0049] Under an inert gas such as a nitrogen atmosphere, add Hypericum perforatum powder and biological enzymes to water, wherein the Hypericum perforatum powder is 100g, and the mass ratio of Hypericum perforatum powder to water is 1:12. The type and amount of added enzymes are shown in Table 1 and Table 2, so as to prepare the Hypericum perforatum enzyme solution; then adjust the pH of the enzyme solution to 5.0, put it in a constant temperature shaker after the adjustment, the shaker speed is 110r...

Embodiment 2

[0057] Embodiment 2: the screening of pH and temperature in enzymolysis process

[0058] Step (1): Processing raw materials

[0059] After washing the flowers and leaves of Hypericum perforatum, put them in a blast drying oven, dry them at a temperature of 35°C until the water content is less than 10%, then crush them with high speed shearing, sieve, and collect the parts with a particle size of 100-200 mesh , to obtain Hypericum perforatum powder.

[0060] Step (2): Enzyme treatment

[0061] Under inert gas such as nitrogen atmosphere, add Hypericum perforatum powder and compound enzyme to water, wherein Hypericum perforatum powder is 100g, the mass ratio of Hypericum perforatum powder and water is 1:12, and described compound enzyme is cellulase and pectinase The mixture, wherein the mass ratio of cellulase and pectinase is 1:7, the mass ratio of the sum of the quality of the compound enzyme and Hypericum perforatum powder is 4:100, thereby preparing Hypericum perforatum...

Embodiment 3

[0069] Embodiment 3: the investigation of the kind of ultrasonic extraction solvent, solid-liquid ratio and alkali consumption

[0070] Step (1): Processing raw materials

[0071] After washing the flowers and leaves of Hypericum perforatum, put them in a blast drying oven, dry them at a temperature of 35°C until the water content is less than 10%, then crush them with high speed shearing, sieve, and collect the parts with a particle size of 100-200 mesh , to obtain Hypericum perforatum powder.

[0072] Step (2): Enzyme treatment

[0073] Under inert gas such as nitrogen atmosphere, add Hypericum perforatum powder and compound enzyme to water, wherein Hypericum perforatum powder is 100g, the mass ratio of Hypericum perforatum powder and water is 1:12, and described compound enzyme is cellulase and pectinase The mixture, wherein the mass ratio of cellulase and pectinase is 1:7, the mass ratio of the sum of the quality of the compound enzyme and Hypericum perforatum powder i...

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Abstract

The invention relates to a method for extracting hypericin from Hypericum perforatum. The method sequentially comprises the steps of enzyme treatment, ultrasonic assisted solvent extraction, and macro-porous adsorption resin separation and purification, and hypericin is efficiently extracted under optimized technological conditions, so the content of hypericin in obtained extract is improved. The extraction method has the advantages of easily available raw material, simple operation, low extraction cost, controllable production cost and wide industrial application prospect.

Description

technical field [0001] The invention relates to an extraction of plant active ingredients, in particular to an extraction method for extracting hypericin medicinal active ingredients from Hypericum perforatum, belonging to the technical field of extraction of medicinal active ingredients. Background technique [0002] Hypericum perforatum contains a variety of biologically active substances, and the types of detectable active compounds are mainly divided into: Naphtodianthrones, Flavonoids, Phloroglucinols, Procyanidins ), Tannins (Tannins), etc. [0003] Among them, Naphtodianthrones (Naphtodianthrones) are representative chemical components of this type of plants, which include: Hypericin (Hypericin), Pseudohypericin (Pseudohypericin), Protohypericin (Protohypercin), Protopseudohypericin and Cyclopseudohypericin. [0004] After a lot of research, it was found that hypericin and pseudohypericin have certain inhibitory and killing activities on herpes simplex virus-1 and -...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/36C07C46/10
Inventor 张锋司红康王启家王贤军吴成柱
Owner 陕西笃诚医药科技有限公司
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